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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
08/11/1995 |
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Data da última atualização: |
08/11/1995 |
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Autoria: |
XIE, W. S.; HU, J. S. |
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Afiliação: |
Department of Plant Pathology, University of Hawaii, Honolulu 96822. |
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Título: |
Molecular cloning, sequence analysis, and detection of banana bunchy top virus in Hawaii. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Phytopathology, v.85, n.3, p.339-347, 1995. |
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ISSN: |
0331-949x |
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Idioma: |
Inglês |
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Conteúdo: |
The Hawaii isolate of banana bunchy top virus (BBTV) was purified frominfected banana cultivar Williams. Three single-stranded DNA (ssDNA) components were cloned and sequenced; they were named component 1,3, and 4, respectively. Component is 1,110 nucleotides in length and shares98% nucleotide sequence identity with the BBTV DNA component 1 of the Australian isolate. this component contains two open reading franmes (ORF) capable of encoding a protein of 33.5 kDa, which may function as a replicase, and a protein about 15.2 kDa, with unknown functions. Component 3 is 1,057 nucleotides in lenght and does not contain any ORFs larger than 10 kDa. Component 4 is 1,017 nucleotides in length and potentially encodes a protein of 18.9 kDa. All three ssDNA components sh are the same stem-loop sequence and have a conserved noncoding region.the sequence of each of these three components is different from that of BBTV DNA components of two Taiwanese isolates. BBTV-specific clones were used in dot-blot hybridization assays for detection of BBTV in plants using radioactive and nonradioactive probes. A polymerase chain reaction (PCR) assay was developed for detection of BBTV in banana samples and single aphids. Dot-blot hybridization assays were as sensitive as enzyme-linked immunosorbent assay (ELISA), while PCR was 1,000 times more sensitive than dot blot and ELISA assays for detedtion of BBTV in banana. |
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Palavras-Chave: |
Geminiviruses; NTP-binding motif. |
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Thesaurus Nal: |
Coconut foliar decay virus; Subterranean clover stunt virus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01972naa a2200193 a 4500
001 1634113
005 1995-11-08
008 1995 bl uuuu u00u1 u #d
022 $a0331-949x
100 1 $aXIE, W. S.
245 $aMolecular cloning, sequence analysis, and detection of banana bunchy top virus in Hawaii.
260 $c1995
520 $aThe Hawaii isolate of banana bunchy top virus (BBTV) was purified frominfected banana cultivar Williams. Three single-stranded DNA (ssDNA) components were cloned and sequenced; they were named component 1,3, and 4, respectively. Component is 1,110 nucleotides in length and shares98% nucleotide sequence identity with the BBTV DNA component 1 of the Australian isolate. this component contains two open reading franmes (ORF) capable of encoding a protein of 33.5 kDa, which may function as a replicase, and a protein about 15.2 kDa, with unknown functions. Component 3 is 1,057 nucleotides in lenght and does not contain any ORFs larger than 10 kDa. Component 4 is 1,017 nucleotides in length and potentially encodes a protein of 18.9 kDa. All three ssDNA components sh are the same stem-loop sequence and have a conserved noncoding region.the sequence of each of these three components is different from that of BBTV DNA components of two Taiwanese isolates. BBTV-specific clones were used in dot-blot hybridization assays for detection of BBTV in plants using radioactive and nonradioactive probes. A polymerase chain reaction (PCR) assay was developed for detection of BBTV in banana samples and single aphids. Dot-blot hybridization assays were as sensitive as enzyme-linked immunosorbent assay (ELISA), while PCR was 1,000 times more sensitive than dot blot and ELISA assays for detedtion of BBTV in banana.
650 $aCoconut foliar decay virus
650 $aSubterranean clover stunt virus
653 $aGeminiviruses
653 $aNTP-binding motif
700 1 $aHU, J. S.
773 $tPhytopathology$gv.85, n.3, p.339-347, 1995.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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