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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
20/07/2017 |
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Data da última atualização: |
20/07/2017 |
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Autoria: |
MURDOCH, R. N.; WHITE, I. G. |
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Título: |
Studies of the stimulating effect of bicarbonate on the metabolism of ram spermatozoa. |
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Ano de publicação: |
1971 |
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Fonte/Imprenta: |
Journal of Reproduction and Development, v. 25, n. 2, p. 231-242, May. 1971. |
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Idioma: |
Inglês |
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Conteúdo: |
The metabolism of washed ram spermatozoa has been studied in the presence and absence of low levels of bicarbonate (6 mM) and CO2 (2%). Although bicarbonate stimulated the O2 consumption of ram spermatozoa in the presence of glucose, acetate or lactate, its most profound effect was on glycolysis and it greatly stimulated the rate of glucose breakdown to lactic acid under both aerobic and anaerobic conditions. Low concentrations of potassium (5 mM) enhanced the stimulating effect of bicarbonate on glycolysis but, in its absence, bicarbonate greatly depressed O2 uptake. Bicarbonate failed to stimulate the conversion of glucose to 6-phospho-glucose when sonicated spermatozoa were incubated in the presence of ATP and sodium fluoride, or the conversion of pyruvate to lactate in intact spermatozoa. This suggests that reactions catalysed by glucokinase and lactic dehydrogenase are not sites at which bicarbonate acts to enhance glycolysis. However, bicarbonate did stimulate the conversion of phosphoenol pyruvate to lactic acid when spermatozoa were incubated under anaerobic conditions in the absence of added ADP; this indicates that the reaction catalysed by pyruvate kinase in ram spermatozoa may be at least one site at which bicarbonate acts to stimulate glycolysis. Although the addition of ADP (5 mM) greatly increased the conversion of phosphoenol pyruvate to lactic acid, it obliterated the stimulating effect of bicarbonate. |
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Palavras-Chave: |
Drug effects; Entities; L-Lactate Dehydrogenase; Pyruvates. |
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Thesagro: |
ATP. |
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Thesaurus Nal: |
Acetates; Bicarbonates; Carbon Dioxide; enzymology; glucokinase; Glycolysis; Lactates; Pharmacology; Physiology; Pyruvate Kinase; Reproduction; Sheep; Spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02357naa a2200349 a 4500
001 2072952
005 2017-07-20
008 1971 bl uuuu u00u1 u #d
100 1 $aMURDOCH, R. N.
245 $aStudies of the stimulating effect of bicarbonate on the metabolism of ram spermatozoa.
260 $c1971
520 $aThe metabolism of washed ram spermatozoa has been studied in the presence and absence of low levels of bicarbonate (6 mM) and CO2 (2%). Although bicarbonate stimulated the O2 consumption of ram spermatozoa in the presence of glucose, acetate or lactate, its most profound effect was on glycolysis and it greatly stimulated the rate of glucose breakdown to lactic acid under both aerobic and anaerobic conditions. Low concentrations of potassium (5 mM) enhanced the stimulating effect of bicarbonate on glycolysis but, in its absence, bicarbonate greatly depressed O2 uptake. Bicarbonate failed to stimulate the conversion of glucose to 6-phospho-glucose when sonicated spermatozoa were incubated in the presence of ATP and sodium fluoride, or the conversion of pyruvate to lactate in intact spermatozoa. This suggests that reactions catalysed by glucokinase and lactic dehydrogenase are not sites at which bicarbonate acts to enhance glycolysis. However, bicarbonate did stimulate the conversion of phosphoenol pyruvate to lactic acid when spermatozoa were incubated under anaerobic conditions in the absence of added ADP; this indicates that the reaction catalysed by pyruvate kinase in ram spermatozoa may be at least one site at which bicarbonate acts to stimulate glycolysis. Although the addition of ADP (5 mM) greatly increased the conversion of phosphoenol pyruvate to lactic acid, it obliterated the stimulating effect of bicarbonate.
650 $aAcetates
650 $aBicarbonates
650 $aCarbon Dioxide
650 $aenzymology
650 $aglucokinase
650 $aGlycolysis
650 $aLactates
650 $aPharmacology
650 $aPhysiology
650 $aPyruvate Kinase
650 $aReproduction
650 $aSheep
650 $aSpermatozoa
650 $aATP
653 $aDrug effects
653 $aEntities
653 $aL-Lactate Dehydrogenase
653 $aPyruvates
700 1 $aWHITE, I. G.
773 $tJournal of Reproduction and Development$gv. 25, n. 2, p. 231-242, May. 1971.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| 1. |  | AMARAL, R. dos S.; MACIEL, D. C.; PEREIRA, L. L.; PASSOS, A. M. A. dos; NETTO, D. A. M. Semillas de Brachiaria ruziziensis en diferentes tiempos y niveles de mezcla con urea para la formación de pasto. Enciclopedia Biosfera, Goiânia, v. 16, n. 30, p. 583-592, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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