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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
24/07/2008 |
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Data da última atualização: |
24/07/2008 |
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Autoria: |
LAI, E. -M.; SHIH, H. -W.; WEN, S. -R.; CHENG, M. -W.; HWANG, H. -H.; CHIU, S. -H. |
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Título: |
Proteomic analysis of Agrobacterium tumefaciens response to the vir gene inducer acetosyringone. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Proteomics, Weinheim, v. 6, n. 14, p. 4130-4136, jul. 2006. |
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Idioma: |
Inglês |
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Conteúdo: |
Agrobacterium tumefaciens causes crown gall disease in a wide range of plants by transforming plants through the transfer and integration of its transfered DNA (T-DNA) into the host genome. In the present study, we used two-dimensional gel electrophoresis to examine the protein expression profiles of A. tumefaciens in response to the phenolic compound acetosyringone (AS), a known plant-released virulence (vir) gene induced. Using mass spectrometry, we identified 11 proteins consisting of 9 known AS-induced Vir proteins and 2 newly discovered AS-induced proteins, an unknow protein Y4mC (Atu6161) and a small heat shock protein HspL (Atu388&). Further expression analysis revealed that the AS-induced expression of Y4mC and HspL is regulated by the VirA/VirG two-component system. This report presents the first proteomics study successfully identifying both known and new AS-induced proteins that are implicated in Agrobacterium virulence. |
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Thesagro: |
Agrobacterium Tumefaciens. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01510naa a2200193 a 4500
001 1623102
005 2008-07-24
008 2006 bl --- 0-- u #d
100 1 $aLAI, E. -M.
245 $aProteomic analysis of Agrobacterium tumefaciens response to the vir gene inducer acetosyringone.
260 $c2006
520 $aAgrobacterium tumefaciens causes crown gall disease in a wide range of plants by transforming plants through the transfer and integration of its transfered DNA (T-DNA) into the host genome. In the present study, we used two-dimensional gel electrophoresis to examine the protein expression profiles of A. tumefaciens in response to the phenolic compound acetosyringone (AS), a known plant-released virulence (vir) gene induced. Using mass spectrometry, we identified 11 proteins consisting of 9 known AS-induced Vir proteins and 2 newly discovered AS-induced proteins, an unknow protein Y4mC (Atu6161) and a small heat shock protein HspL (Atu388&). Further expression analysis revealed that the AS-induced expression of Y4mC and HspL is regulated by the VirA/VirG two-component system. This report presents the first proteomics study successfully identifying both known and new AS-induced proteins that are implicated in Agrobacterium virulence.
650 $aAgrobacterium Tumefaciens
700 1 $aSHIH, H. -W.
700 1 $aWEN, S. -R.
700 1 $aCHENG, M. -W.
700 1 $aHWANG, H. -H.
700 1 $aCHIU, S. -H.
773 $tProteomics, Weinheim$gv. 6, n. 14, p. 4130-4136, jul. 2006.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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