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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
28/09/2020 |
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Data da última atualização: |
07/12/2020 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
CARNEIRO, M. T.; PEREZ, D. V.; FEITOSA, R. C.; WASSERMAN, J. C. |
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Afiliação: |
MARCOS TAVARES CARNEIRO, UFF/FIOCRUZ; DANIEL VIDAL PEREZ, CNPS; RENATO CASTIGLIA FEITOSA, FIOCRUZ; JULIO CESAR WASSERMAN, UFF. |
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Título: |
Separation of Escherichia coli from natural samples for identification of sources and microcosm inoculation. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Brazilian Journal Of Microbiology, v. 51, p. 2015-2020, 2020. |
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DOI: |
https://doi.org/10.1007/s42770-020-00374-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Obtaining uncultured Escherichia coli from natural waters is an important step in the study of microbes in the environment, which are critical for bacterial decay and microbial source tracking. The quality of the samples used can influence the assays, because high contaminant concentrations, differing cell ages, and physiologic states can impair results. The proposed separation is based on a three-step filtration method applied to replicates of seven samples from a sewage plant affluent, collected in different periods. Aliquots of the leachate were inoculated into microcosms, aiming to observe the cultivability of the cells. The assay resulted in colimetry values ranging between 104 and 105 cells. In the leachate, averages of 1.05% of total coliforms and 1.10% of Escherichia coli were recovered from original samples. Although enduring unfavorable temperatures, salinities, and nutritional conditions, the inoculated microcosm populations grew approximately 310 times after 24 h. The final leachate contained cultivable cells in appropriate physiological states and quantities for inoculum in microcosm sets. The bacteria obtained from the leachate were also appropriate for surveys of microbial source tracking, because, in the developed procedure, organisms were separated from contaminants, while cell concentrations were sufficient for inocula. |
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Thesagro: |
Águas Residuais; Bactéria; Escherichia Coli; Rastreamento. |
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Thesaurus Nal: |
Strains; Wastewater. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218815/1/Separation-of-Escherichia-coli-from-natural-samples-2020.pdf
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Marc: |
LEADER 02106naa a2200241 a 4500
001 2125143
005 2020-12-07
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-020-00374-2$2DOI
100 1 $aCARNEIRO, M. T.
245 $aSeparation of Escherichia coli from natural samples for identification of sources and microcosm inoculation.$h[electronic resource]
260 $c2020
520 $aObtaining uncultured Escherichia coli from natural waters is an important step in the study of microbes in the environment, which are critical for bacterial decay and microbial source tracking. The quality of the samples used can influence the assays, because high contaminant concentrations, differing cell ages, and physiologic states can impair results. The proposed separation is based on a three-step filtration method applied to replicates of seven samples from a sewage plant affluent, collected in different periods. Aliquots of the leachate were inoculated into microcosms, aiming to observe the cultivability of the cells. The assay resulted in colimetry values ranging between 104 and 105 cells. In the leachate, averages of 1.05% of total coliforms and 1.10% of Escherichia coli were recovered from original samples. Although enduring unfavorable temperatures, salinities, and nutritional conditions, the inoculated microcosm populations grew approximately 310 times after 24 h. The final leachate contained cultivable cells in appropriate physiological states and quantities for inoculum in microcosm sets. The bacteria obtained from the leachate were also appropriate for surveys of microbial source tracking, because, in the developed procedure, organisms were separated from contaminants, while cell concentrations were sufficient for inocula.
650 $aStrains
650 $aWastewater
650 $aÁguas Residuais
650 $aBactéria
650 $aEscherichia Coli
650 $aRastreamento
700 1 $aPEREZ, D. V.
700 1 $aFEITOSA, R. C.
700 1 $aWASSERMAN, J. C.
773 $tBrazilian Journal Of Microbiology$gv. 51, p. 2015-2020, 2020.
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Registro original: |
Embrapa Solos (CNPS) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Ocidental. Para informações adicionais entre em contato com cpaa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
20/02/2017 |
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Data da última atualização: |
29/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MATSCHULLAT, J.; LIMA, R. M. B. de. |
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Afiliação: |
Jorg Matschullat, Interdisciplinary Environmental Research Centre; ROBERVAL MONTEIRO BEZERRA DE LIMA, CPAA. |
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Título: |
Examining soil/ecosystem respiration in the Amazon basin. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Environmental Earth Sciences, v. 76, p. 141, Feb. 2017. |
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DOI: |
10.1007/s12665-016-6370-2 |
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Idioma: |
Inglês |
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Palavras-Chave: |
Ecosystem. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00451naa a2200145 a 4500
001 2064861
005 2017-03-29
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1007/s12665-016-6370-2$2DOI
100 1 $aMATSCHULLAT, J.
245 $aExamining soil/ecosystem respiration in the Amazon basin.$h[electronic resource]
260 $c2017
653 $aEcosystem
700 1 $aLIMA, R. M. B. de
773 $tEnvironmental Earth Sciences$gv. 76, p. 141, Feb. 2017.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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