Data/hora: 07/06/2024 08:31:30

Biblioteca(s): | Embrapa Pantanal.
Data corrente: | 23/08/1995
Data da última atualização: | 21/01/2011
Autoria: | WARNER, A. C. L.
Título: | Criteria for establishing the validity of in vitro studies with rumen micro-organisms in so- called artificial rumen systems.
Ano de publicação: | 1956
Fonte/Imprenta: | Journal of General Microbiology, v.14, p.733-748, 1956.
Idioma: | Inglês
Conteúdo: | Several criteria of normal rumen function which can be applied to in vitro studies with the whole rumen microbial population are suggested. These include: the maintenance of numbers and normal appearance of the bacteria, selonomonads and protozoa of the rumen; the maintenance of normal rates of digestion of celluloses, starch and protein, and of normal interactions between these: the ability to predict quantitative results in vivo. An artificial rumen was constructed, consisting of a cellpphan sac conyaining rumen liquor and  substrate dialysing against a complex mineral solution whose composition was based on that found in rumen liquor, the whole being incubated at 30 in an atmosphere on nitrogen and carbon dioxide. This system was shown to meet the criteria which are suggested, with reasonable success for period of about 8 hr.; over longer periods an increasing failure to meet the biological criteria was seen. For the microbial population to remain normal in numbers and activity it was shown to be necessary to use as test substrate in vitro only substances similar to the diet fed to the animal from which the rumen liquor inoculum was taken.
Palavras-Chave: | Artificial systems; Micro-organismo; Micro-organisms; Microbial population; Populacao microbiano; Sistema artificial.
Thesagro: | Bactéria; Rúmen.
Categoria do assunto: | --
Registro original: | Embrapa Pantanal (CPAP)
Exemplares: | CPAP - (29332-1) - ADD - SP - -- - SP0952 -  - 0952 - DIS