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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
07/11/2022 |
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Data da última atualização: |
07/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MATOS, M. K. da S.; BENKO-ISEPPON, A. M.; BEZERRA-NETO, J. P.; FERREIRA-NETO, J. R. C.; WANG, Y.; LIU, H.; PANDOLFI, V.; AMORIM, L. L. B.; WILLADINO, L.; AMORIM, T. C. do V.; KIDO, E. A.; VIANELLO, R. P.; TIMKO, M. P.; BRASILEIRO-VIDAL, A. C. |
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Afiliação: |
MITALLE KAREN DA SILVA MATOS, UNIVERSIDADE FEDERAL DE PERNAMBUCO; ANA MARIA BENKO-ISEPPON, UNIVERSIDADE FEDERAL DE PERNAMBUCO; JOAO PACIFICO BEZERRA-NETO, UNIVERSIDADE FEDERAL DE PERNAMBUCO; JOSE RIBAMAR COSTA FERREIRA-NETO, UNIVERSIDADE FEDERAL DE PERNAMBUCO; YU WANG, UNIVERSITY OF VIRGINA; HAI LIU, UNIVERSITY OF VIRGINIA; VALESCA PANDOLFI, UNIVERSIDADE FEDERAL DE PERNAMBUCO; LIDIANE LINDINALVA BARBOSA AMORIM, UNIVERSIDADE FEDERAL DE PERNAMBUCO; LILIA WILLADINO, UNIVERSIDADE FEDERAL DE PERNAMBUCO; THIALISSON CAACI DO VALE AMORIM, UNIVERSIDADE FEDERAL DE PERNAMBUCO; EDERSON AKIO KIDO, UNIVERSIDADE FEDERAL DE PERNAMBUCO; ROSANA PEREIRA VIANELLO, CNPAF; MICHAEL P. TIMKO, UNIVERSITY OF VIRGINIA; ANA CHRISTINA BRASILEIRO-VIDAL, UNIVERSIDADE FEDERAL DE PERNAMBUCO. |
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Título: |
The WRKY transcription factor family in cowpea: Genomic characterization and transcriptomic profiling under root dehydration. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Gene, v. 823, 146377, May 2022. |
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ISSN: |
0378-1119 |
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DOI: |
https://doi.org/10.1016/j.gene.2022.146377 |
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Idioma: |
Inglês |
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Conteúdo: |
Cowpea [Vigna unguiculata (L.) Walp.] is one of the most tolerant legume crops to drought and salt stresses. WRKY transcription factor (TF) family members stand out among plant transcriptional regulators related to abiotic stress tolerance. However, little information is currently available on the expression of the cowpea WRKY gene family (VuWRKY) in response to water deficit. Thus, we analyzed genomic and transcriptomic data from cowpea to identify VuWRKY members and characterize their structure and transcriptional response under root dehydration stress. Ninety-two complete VuWRKY genes were found in the cowpea genome based on their domain characteristics. They were clustered into three groups: I (15 members), II (58), and III (16), while three genes were unclassified. Domain analysis of the encoded proteins identified four major variants of the conserved heptapeptide motif WRKYGQK. In silico analysis of VuWRKY gene promoters identified eight candidate binding motifs of cis-regulatory elements, regulated mainly by six TF families associated with abiotic stress responses. Ninety-seven VuWRKY modulated splicing variants associated with 55 VuWRKY genes were identified via RNA-Seq analysis available at the Cowpea Genomics Consortium (CpGC) database. qPCR analyses showed that 22 genes are induced under root dehydration, with VuWRKY18, 21, and 75 exhibiting the most significant induction levels. Given their central role in activating signal transduction cascades in abiotic stress response, the data provide a foundation for the targeted modification of specific VuWRKY family members to improve drought tolerance in this important climate-resilient legume in the developing world and beyond. MenosCowpea [Vigna unguiculata (L.) Walp.] is one of the most tolerant legume crops to drought and salt stresses. WRKY transcription factor (TF) family members stand out among plant transcriptional regulators related to abiotic stress tolerance. However, little information is currently available on the expression of the cowpea WRKY gene family (VuWRKY) in response to water deficit. Thus, we analyzed genomic and transcriptomic data from cowpea to identify VuWRKY members and characterize their structure and transcriptional response under root dehydration stress. Ninety-two complete VuWRKY genes were found in the cowpea genome based on their domain characteristics. They were clustered into three groups: I (15 members), II (58), and III (16), while three genes were unclassified. Domain analysis of the encoded proteins identified four major variants of the conserved heptapeptide motif WRKYGQK. In silico analysis of VuWRKY gene promoters identified eight candidate binding motifs of cis-regulatory elements, regulated mainly by six TF families associated with abiotic stress responses. Ninety-seven VuWRKY modulated splicing variants associated with 55 VuWRKY genes were identified via RNA-Seq analysis available at the Cowpea Genomics Consortium (CpGC) database. qPCR analyses showed that 22 genes are induced under root dehydration, with VuWRKY18, 21, and 75 exhibiting the most significant induction levels. Given their central role in activating signal transduction cascades in abiotic stress... Mostrar Tudo |
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Palavras-Chave: |
QPCR; RNA-Seq. |
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Thesagro: |
Seca; Vigna Unguiculata. |
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Thesaurus Nal: |
Abiotic stress; Cowpeas; Drought tolerance; Gene expression. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02849naa a2200397 a 4500
001 2148067
005 2022-11-07
008 2022 bl uuuu u00u1 u #d
022 $a0378-1119
024 7 $ahttps://doi.org/10.1016/j.gene.2022.146377$2DOI
100 1 $aMATOS, M. K. da S.
245 $aThe WRKY transcription factor family in cowpea$bGenomic characterization and transcriptomic profiling under root dehydration.$h[electronic resource]
260 $c2022
520 $aCowpea [Vigna unguiculata (L.) Walp.] is one of the most tolerant legume crops to drought and salt stresses. WRKY transcription factor (TF) family members stand out among plant transcriptional regulators related to abiotic stress tolerance. However, little information is currently available on the expression of the cowpea WRKY gene family (VuWRKY) in response to water deficit. Thus, we analyzed genomic and transcriptomic data from cowpea to identify VuWRKY members and characterize their structure and transcriptional response under root dehydration stress. Ninety-two complete VuWRKY genes were found in the cowpea genome based on their domain characteristics. They were clustered into three groups: I (15 members), II (58), and III (16), while three genes were unclassified. Domain analysis of the encoded proteins identified four major variants of the conserved heptapeptide motif WRKYGQK. In silico analysis of VuWRKY gene promoters identified eight candidate binding motifs of cis-regulatory elements, regulated mainly by six TF families associated with abiotic stress responses. Ninety-seven VuWRKY modulated splicing variants associated with 55 VuWRKY genes were identified via RNA-Seq analysis available at the Cowpea Genomics Consortium (CpGC) database. qPCR analyses showed that 22 genes are induced under root dehydration, with VuWRKY18, 21, and 75 exhibiting the most significant induction levels. Given their central role in activating signal transduction cascades in abiotic stress response, the data provide a foundation for the targeted modification of specific VuWRKY family members to improve drought tolerance in this important climate-resilient legume in the developing world and beyond.
650 $aAbiotic stress
650 $aCowpeas
650 $aDrought tolerance
650 $aGene expression
650 $aSeca
650 $aVigna Unguiculata
653 $aQPCR
653 $aRNA-Seq
700 1 $aBENKO-ISEPPON, A. M.
700 1 $aBEZERRA-NETO, J. P.
700 1 $aFERREIRA-NETO, J. R. C.
700 1 $aWANG, Y.
700 1 $aLIU, H.
700 1 $aPANDOLFI, V.
700 1 $aAMORIM, L. L. B.
700 1 $aWILLADINO, L.
700 1 $aAMORIM, T. C. do V.
700 1 $aKIDO, E. A.
700 1 $aVIANELLO, R. P.
700 1 $aTIMKO, M. P.
700 1 $aBRASILEIRO-VIDAL, A. C.
773 $tGene$gv. 823, 146377, May 2022.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 27 | |
| 3. |  | SUMYANTO, J.; DAYAN, F. E.; CERDEIRA, A. L.; WANG, Y. H.; KHAN, I. A.; MORAES, R. M. Oligofructans content and yield of yacon (Smallanthus sonchifolius) cultivated in Mississippi. Scientia Horticulturae, Amsterdam, v. 148, p. 83-88, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio Ambiente. |
|    |
| 4. | | ELLINGSON, J. S.; WANG, Y.; LAYTON, S.; ZANELLA, J. R. C.; ROOF, M. B.; FAABERG, K. S. Vaccine efficacy of porcine reproductive and respiratory syndrome virus chimeras. Vaccine, v. 28, n. 14, p. 2679-2686, 2010. Projeto/Plano de Ação: 03.09.00.046.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 5. | | ANTONIOLLI, L. R.; SHARROCK, K. R.; CLARK, C. J.; MCDONALD, R. M.; SEELYE, R. J.; WANG, Y. M.; FEISST, N. P. Amadurecimento e qualidade pós-colheita de kiwis gold3. Bento Gonçalves, RS: Embrapa Uva e Vinho, 2015. (Embrapa Uva e Vinho. Circular Técnica, 121).| Biblioteca(s): Embrapa Uva e Vinho. |
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| 6. | | MAGALHAES, J. V.; GARVIN, D. F.; WANG, Y. H.; SORRELLS, M. E.; KLEIN, P. E.; SCHAFFERT, R. E.; LI, L.; KOCHIAN, L. V. Comparative mapping of a major aluminum tolerance gene in sorghum and other species in the poaceae. Genetics, Maryland, v. 167, n. 4, p. 1905-1914, 2004.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 8. | | RIBEIRO JUNIOR, G. O.; GONÇALVES, L. C.; PEREIRA, L. G. R.; CHAVES, A. V.; WANG, Y.; BEAUCHEMIN, K. A.; McALLISTER, T. A. Effect of fibrolytic enzymes added to a Andropogon gayanus grass silage-concentrate diet on rumen fermentation in batch cultures and the artificial rumen (Rusitec). Animal, v. 9, n. 7, p. 1153-1162, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | LIU, J.; MAGALHAES, J. V. de; GUIMARAES, C. T.; LANA, U. G. de P.; HOEKENGA, O.; SHAFF, J.; PINEROS, M.; WANG, Y.; SCHAFFERT, R. E.; KOCHIAN, L. Molecular cloning and characterization of AltSB, a major aluminum tolerance gene in sorghum. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 15., 2007, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 10. | | ZHU, H.; ZHANG, H.; WANG, Y.; CIREN, D.; DONG, H.; WU, Q.; REHMAN, M. U.; NABI, F.; MEHMOOD, K.; LI, J. Phylogenetic and pathotypic characterization of newcastle disease virus in Tibetan chickens, China. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 1, p. 37-40, janeiro 2018.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 11. | | LANA, U. G. P.; BAHIA, G. S.; GUIMARAES, C. T.; ALVES, V. M. C.; SCHAFFERT, R. E.; WANG, Y.; KLEIN, P. E.; CARVALHO, D. C.; MAGALHAES, J. V. Mapeamento de alta resolução do gene de tolerância ao alumínio ALTsb em sorgo In: CONGRESSO BRASILEIRO DE GENÉTICA, 49., 2003, Águas de Lindóia. A dupla hélice do DNA: [resumos]. Ribeirão Preto: Sociedade Brasileira de Genética, 2003. p. 639.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 12. | | WANG, Y.; ZIV, G.; ADAMI, M.; ALMEIDA, C. A. de; ANTUNES, J. F. G.; COUTINHO, A. C.; ESQUERDO, J. C. D. M.; GOMES, A. R.; GALBRAITH, D. Upturn in secondary forest clearing buffers primary forest loss in the Brazilian Amazon. Nature sustainability, v. 3, n. 4, Apr. 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 13. | | MAGALHAES, J. V. de; WANG, Y.; KLEIN, P. E.; GUIMARAES, C. T.; LANA, U. G. de P.; SCHAFFERT, R. E.; ALVES, V. M. C.; HOEKENGA, O. A.; CARNEIRO, N. P.; CARNEIRO, A. A.; KOCHIAN, L. V. Clonagem do principal gene de tolerância ao alumínio em sorgo e sua exploração no aumento da produção de grãos em solos ácidos. In: SIMPÓSIO SOBRE INOVAÇÃO E CRIATIVIDADE CIENTÍFICA NA EMBRAPA, 1., 2008, Brasília, DF. Resumos... Brasília, DF: Embrapa, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 14. | | LINS, T. O. J. D'A.; TERRY, S. A.; SILVA, R. R.; PEREIRA, L. G. R.; JANCEWICZ, L. J.; HE, M. L.; WANG, Y.; McALLISTER, T. A.; CHAVES, A. V. Effects of the inclusion of Moringa oleifera seed on rumen fermentation and methane production in a beef cattle diet using the rumen simulation technique (Rusitec). Animal, v. 13, n. 2, p. 283-291, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | MAGALHAES, J. V.; GUIMARAES, C. T.; SCHAFFERT, R. E.; CANIATO, F. F.; LANA, U. P.; ALVES, V. M. C.; KLEIN, P. E.; WANG, Y.; LIU, J.; KOCHIAN, L. V. Molecular genetic studies of aluminum tolerance in sorghum: towards the positional cloning of a major Al tolerance gene and diversity for Al tolerance control in the genus sorghum. In: GRANTEE CONFERENCE FROM PARTNERSHIPS TO COMMUNITY: THE ARC OF CHANGE IN THE CCRP, 2004, Vaals, The Netherlands. [Proceedings]. [Minneapolis]: The McKnight Foundation, 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 16. | | MAGALHAES, J. V. de; KOCHIAN, L.; LIU, J.; GUIMARAES, C.; LANA, U.; ALVES, V.; WANG, Y.; SCHAFFERT, R. E.; HOEKENGA, O.; PINEROS, M.; SHAFF, J.; KLEIN, P.; CARNEIRO, N.; COELHO, C.; TRICK, H.; CANIATO, F.; KRESOVICH, S.; MITCHELL, S. Positional cloning and association analysis of a mate gene that confers aluminum tolerance in sorghum via the AltSB locus. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 16., 2008, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2008. p. 13.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 17. | | MAGALHAES, J. V. de; LIU, J.; ALVES, V. M. C.; GUIMARAES, C. T.; WANG, Y.; LANA, U. G. de P.; SCHAFFERT, R. E.; KLEIN, P.; HOEKENGA, O.; PINEROS, M.; KOCHIAN, L. Positional cloning and characterization of AltSB, a major aluminum tolerance gene in sorghum: toward the identification of the molecular and physiological basis of allelic effects. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 15., 2007, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 18. | | MAGALHAES, J.; KOCHIAN L.; LIU, J.; GUIMARAES, C.; LANA, U.; ALVES, V.; WANG, Y.; SCHAFFERT, R.; HOEKENGA, O.; PINEROS, M.; KLEIN, P.; CARNEIRO, N.; COELHO, C.; TRICK, H.; CANIATO, F.; KRESOVOCH, S.; MITCHELL, S. Positional clonning and association analysis of a gene that confers aluminum tolerance in Sorghum via the ALTsb locus. In: REUNIÓN LATINOAMERICANA, 13.; REUNIÓN ARGENTINA DE FISIOLOGIA VEGETAL, 27., 2008, Rosario. Resúmenes de conferencias, simposios y trabajos. Rosario: Asociación Argentina de Fisiologia Vegetal, 2008. p. 13.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 19. | | MAGALHAES, J. V. de; LIU, J.; GUIMARAES, C. T.; LANA, U. G. de P.; ALVES, V. M. C.; WANG, Y-H.; SCHAFFERT, R. E.; HOEKENGA, O. A.; PINEROS, M. A.; SHAFF, J. E.; KLEIN, P. E.; CARNEIRO, N. P.; COELHO, C. M.; TRICK, H. N.; KOCHIAN, L. V. A gene in the multidrug and toxic compound extrusion (MATE) family confers aluminum tolerance in sorghum. Nature Genetics, New York, v. 39, n. 9, p. 1156-1161, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 20. | | HE, X.; BRUM, P. A. R. de; CHUKWUDEBE, A.; PRIVALLE, L.; REED, A.; WANG, Y.; ZHOU, C.; WANG, C.; LU, J.; HUANG, K.; CONTRI, D.; NAKATANI, A.; AVILA, V. S. de; KLEIN, C. H.; LIMA, G. J. M. M. de; LIPSCOMB, E. A. Rat and poultry feeding studies with soybean meal produced from imidazolinone-tolerant (CV127) soybeans. Food and Chemical Toxicology, v. 88, p. 48-56, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 27 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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