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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
18/09/2018 |
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Data da última atualização: |
18/09/2018 |
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Autoria: |
WALLACE, J. M; HELLIWELL, R.; MORGAN, P. J. |
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Título: |
Autoradiographical localization of oxytocin binding sites on ovine oviduct and uterus throughout the oestrous cycle. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 3, n. 2, p. 127-135, 1991. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: A highly specific oxytocin receptor ligand, 125I-labelled d(CH2)5[Tyr(Me)2,Thr4,Tyr-NH9(2)] vasotocin (125I-OTA), was used to localize high affinity oxytocin receptors in ovine uterine and oviduct tissues throughout the oestrous cycle. The pattern of binding revealed by in vitro autoradiography correlated well with the results of the homogenate receptor assays using the same ligand and with previous binding assays using the tritiated ligand. At oestrus, specific 125I-OTA binding was evident on the luminal epithelium of the caruncular and intercaruncular regions, on the epithelial cells lining the secretory uterine glands and in the stroma underlying the caruncular epithelium. In the myometrium diffuse labelling was evident in the outer longitudinal smooth muscle layer. At Day 4 of the cycle, binding to the stroma was diffuse and virtually absent from the glandular epithelium. No specific binding was evident in either tissue at Day 12 of the luteal phase, but by Day 14, prior to the decrease in peripheral progesterone concentrations, binding was again apparent on the luminal epithelium only. Specific binding to the oviduct was localized to the smooth muscle layer of the isthmus region of oestrous ewes and was not detected at any other stage of the oestrous cycle. These studies extend our knowledge of the distribution of oxytocin binding sites in uterine and oviduct tissues throughout the oestrous cycle and suggest that oxytocin has an important role in stimulating oviduct and uterine motility at a time crucial to successful egg collection and/or sperm embryo transport. MenosAbstract: A highly specific oxytocin receptor ligand, 125I-labelled d(CH2)5[Tyr(Me)2,Thr4,Tyr-NH9(2)] vasotocin (125I-OTA), was used to localize high affinity oxytocin receptors in ovine uterine and oviduct tissues throughout the oestrous cycle. The pattern of binding revealed by in vitro autoradiography correlated well with the results of the homogenate receptor assays using the same ligand and with previous binding assays using the tritiated ligand. At oestrus, specific 125I-OTA binding was evident on the luminal epithelium of the caruncular and intercaruncular regions, on the epithelial cells lining the secretory uterine glands and in the stroma underlying the caruncular epithelium. In the myometrium diffuse labelling was evident in the outer longitudinal smooth muscle layer. At Day 4 of the cycle, binding to the stroma was diffuse and virtually absent from the glandular epithelium. No specific binding was evident in either tissue at Day 12 of the luteal phase, but by Day 14, prior to the decrease in peripheral progesterone concentrations, binding was again apparent on the luminal epithelium only. Specific binding to the oviduct was localized to the smooth muscle layer of the isthmus region of oestrous ewes and was not detected at any other stage of the oestrous cycle. These studies extend our knowledge of the distribution of oxytocin binding sites in uterine and oviduct tissues throughout the oestrous cycle and suggest that oxytocin has an important role in stimulating o... Mostrar Tudo |
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Thesaurus Nal: |
Angiotensins; Animal physiology; Autoradiography; Blood; Cell membranes; Dosage; Endometrium; Epithelium; Estrus; Fallopian tubes; Metabolism; Myometrium; Pharmacology; Progesterone; Receptors; Sheep; Uterus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02538naa a2200349 a 4500
001 2095968
005 2018-09-18
008 1991 bl uuuu u00u1 u #d
100 1 $aWALLACE, J. M
245 $aAutoradiographical localization of oxytocin binding sites on ovine oviduct and uterus throughout the oestrous cycle.
260 $c1991
520 $aAbstract: A highly specific oxytocin receptor ligand, 125I-labelled d(CH2)5[Tyr(Me)2,Thr4,Tyr-NH9(2)] vasotocin (125I-OTA), was used to localize high affinity oxytocin receptors in ovine uterine and oviduct tissues throughout the oestrous cycle. The pattern of binding revealed by in vitro autoradiography correlated well with the results of the homogenate receptor assays using the same ligand and with previous binding assays using the tritiated ligand. At oestrus, specific 125I-OTA binding was evident on the luminal epithelium of the caruncular and intercaruncular regions, on the epithelial cells lining the secretory uterine glands and in the stroma underlying the caruncular epithelium. In the myometrium diffuse labelling was evident in the outer longitudinal smooth muscle layer. At Day 4 of the cycle, binding to the stroma was diffuse and virtually absent from the glandular epithelium. No specific binding was evident in either tissue at Day 12 of the luteal phase, but by Day 14, prior to the decrease in peripheral progesterone concentrations, binding was again apparent on the luminal epithelium only. Specific binding to the oviduct was localized to the smooth muscle layer of the isthmus region of oestrous ewes and was not detected at any other stage of the oestrous cycle. These studies extend our knowledge of the distribution of oxytocin binding sites in uterine and oviduct tissues throughout the oestrous cycle and suggest that oxytocin has an important role in stimulating oviduct and uterine motility at a time crucial to successful egg collection and/or sperm embryo transport.
650 $aAngiotensins
650 $aAnimal physiology
650 $aAutoradiography
650 $aBlood
650 $aCell membranes
650 $aDosage
650 $aEndometrium
650 $aEpithelium
650 $aEstrus
650 $aFallopian tubes
650 $aMetabolism
650 $aMyometrium
650 $aPharmacology
650 $aProgesterone
650 $aReceptors
650 $aSheep
650 $aUterus
700 1 $aHELLIWELL, R.
700 1 $aMORGAN, P. J.
773 $tReproduction, Fertility and Development$gv. 3, n. 2, p. 127-135, 1991.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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