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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
11/10/1996 |
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Data da última atualização: |
30/04/2009 |
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Autoria: |
WALKER, S. K.; HILL, J. L.; KLEEMANN, D. O.; NANCARROW, C. D. |
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Título: |
Development of ovine embryos in synthetic oviductal fluid containing amino acids at oviductal fluid concentration. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Biology of Reproduction, v.55, n.3, p.703-708, 1996. |
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Idioma: |
Inglês |
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Conteúdo: |
The effects of supplementing synthetic oviductal fluid (SOF) with amino acids, at oviductal fluid concentrations, on the development of ovine in vitro-matured/in vitro-fertilized embryos was examined in three experiments. ln the first, embryo development in SOF, SOF + 2% human serum (HS), SOF + 20% HS, and SOF + BSA, with and without amino acid supplementation, was examined. Development of zygotes to the blastocyst and hatching blastocyst stages was highest in medium containing 20% HS (64.8% and 54.4%, respectively) irrespective of amino acid supplementation. However, supplementation was significantly beneficial in ail other media, with up to 42.1% of zygotes developing into hatching blastocysts. ln these media, supplementation also significantly increased the mean number of nuclei per newly formed blastocyst (up to a mean of 70.8) and reduced the time during which blastocysts formed. Experiment 2 was an examination of the effect on embryo development of three amino acid preparations (oviduct amino acid concentrations vs. Eagle's Basal Medium (BME) essential + Minimum Essential Medium (MEM) nonessential vs. MEM essential + MEM ( nonessential concentrations) and the presence or absence of t BSA. Both the amino acid and BSA treatments significantly influenced the percentage of zygotes that developed to the hatching blastocyst stage but not to the blastocyst stage. The preferred (medium contained amino acids at oviductal fluid concentrations and BSA (54.5 % hatching rate). The amino acid treatments did not significantly influence the mean number of nuclei per newly formed blastocyst, but the addition of BSA had a significant effect (70.7 +- 1.14 vs. 75.7 +- 1.13). ln experiment 3, embryo development to Day 13 was examined after culture in SOF containing amino acids at oviductal fluid concentrations. Embryos were cultured in the presence of either BSA, polyvinyl alcohol (PVA), or no additional supplement and were transferred to recipient ewes on either Day 0 (after in vitro fertilization), 3, or aJ 5. The addition of BSA or PVA had no significant effect, but ili significantly more embryos developed to Day 13 following transfer on Day 0 (60.0%) than on either Day 3 or 5 (overaIl 45.4%). It is concluded that SOF containing oviductal fluid concentra- ce lions of amino acids 1) facilitates the development of a high for percentage (57.5%) of blastocysts, 2) improves embryo morphology compared with that observed in medium containing HS, ef 3) significantly improves hatching rates compared with those obtained in SOF containing commercially available preparations of amino acids, and 4) produces embryos with relatively high levers of viability to Day 13 of pregnancy. MenosThe effects of supplementing synthetic oviductal fluid (SOF) with amino acids, at oviductal fluid concentrations, on the development of ovine in vitro-matured/in vitro-fertilized embryos was examined in three experiments. ln the first, embryo development in SOF, SOF + 2% human serum (HS), SOF + 20% HS, and SOF + BSA, with and without amino acid supplementation, was examined. Development of zygotes to the blastocyst and hatching blastocyst stages was highest in medium containing 20% HS (64.8% and 54.4%, respectively) irrespective of amino acid supplementation. However, supplementation was significantly beneficial in ail other media, with up to 42.1% of zygotes developing into hatching blastocysts. ln these media, supplementation also significantly increased the mean number of nuclei per newly formed blastocyst (up to a mean of 70.8) and reduced the time during which blastocysts formed. Experiment 2 was an examination of the effect on embryo development of three amino acid preparations (oviduct amino acid concentrations vs. Eagle's Basal Medium (BME) essential + Minimum Essential Medium (MEM) nonessential vs. MEM essential + MEM ( nonessential concentrations) and the presence or absence of t BSA. Both the amino acid and BSA treatments significantly influenced the percentage of zygotes that developed to the hatching blastocyst stage but not to the blastocyst stage. The preferred (medium contained amino acids at oviductal fluid concentrations and BSA (54.5 % hatching rate). The ... Mostrar Tudo |
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Palavras-Chave: |
Ovideto. |
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Thesagro: |
Embrião; Ovino; Reprodução. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03285naa a2200205 a 4500
001 1516579
005 2009-04-30
008 1996 bl uuuu u00u1 u #d
100 1 $aWALKER, S. K.
245 $aDevelopment of ovine embryos in synthetic oviductal fluid containing amino acids at oviductal fluid concentration.
260 $c1996
520 $aThe effects of supplementing synthetic oviductal fluid (SOF) with amino acids, at oviductal fluid concentrations, on the development of ovine in vitro-matured/in vitro-fertilized embryos was examined in three experiments. ln the first, embryo development in SOF, SOF + 2% human serum (HS), SOF + 20% HS, and SOF + BSA, with and without amino acid supplementation, was examined. Development of zygotes to the blastocyst and hatching blastocyst stages was highest in medium containing 20% HS (64.8% and 54.4%, respectively) irrespective of amino acid supplementation. However, supplementation was significantly beneficial in ail other media, with up to 42.1% of zygotes developing into hatching blastocysts. ln these media, supplementation also significantly increased the mean number of nuclei per newly formed blastocyst (up to a mean of 70.8) and reduced the time during which blastocysts formed. Experiment 2 was an examination of the effect on embryo development of three amino acid preparations (oviduct amino acid concentrations vs. Eagle's Basal Medium (BME) essential + Minimum Essential Medium (MEM) nonessential vs. MEM essential + MEM ( nonessential concentrations) and the presence or absence of t BSA. Both the amino acid and BSA treatments significantly influenced the percentage of zygotes that developed to the hatching blastocyst stage but not to the blastocyst stage. The preferred (medium contained amino acids at oviductal fluid concentrations and BSA (54.5 % hatching rate). The amino acid treatments did not significantly influence the mean number of nuclei per newly formed blastocyst, but the addition of BSA had a significant effect (70.7 +- 1.14 vs. 75.7 +- 1.13). ln experiment 3, embryo development to Day 13 was examined after culture in SOF containing amino acids at oviductal fluid concentrations. Embryos were cultured in the presence of either BSA, polyvinyl alcohol (PVA), or no additional supplement and were transferred to recipient ewes on either Day 0 (after in vitro fertilization), 3, or aJ 5. The addition of BSA or PVA had no significant effect, but ili significantly more embryos developed to Day 13 following transfer on Day 0 (60.0%) than on either Day 3 or 5 (overaIl 45.4%). It is concluded that SOF containing oviductal fluid concentra- ce lions of amino acids 1) facilitates the development of a high for percentage (57.5%) of blastocysts, 2) improves embryo morphology compared with that observed in medium containing HS, ef 3) significantly improves hatching rates compared with those obtained in SOF containing commercially available preparations of amino acids, and 4) produces embryos with relatively high levers of viability to Day 13 of pregnancy.
650 $aEmbrião
650 $aOvino
650 $aReprodução
653 $aOvideto
700 1 $aHILL, J. L.
700 1 $aKLEEMANN, D. O.
700 1 $aNANCARROW, C. D.
773 $tBiology of Reproduction$gv.55, n.3, p.703-708, 1996.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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