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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
02/02/1996 |
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Data da última atualização: |
02/02/1996 |
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Autoria: |
VU, J. C. V.; NIEDZ, R. P.; YELENOSKY, G. |
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Afiliação: |
U.S. Department of Agriculture, Agricultural Research Service, Agronomy Physiology, Building 164, University of Florida, Gainesville, FL 32611-0840, U.S.A. |
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Título: |
Activities of sucrose metabolism enzymes in glycerol-grown suspension cultures of sweet orange (Citrus Sinensis L. Osbeck). |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Environmental and experimental botany, v. 35, n. 4, 455-463, 1995 |
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Idioma: |
Inglês |
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Conteúdo: |
Vu J.C.V., Niedz R.P. and Yelenosky G. Activities of sucrose metabolism enzymes in glycerol-grown suspension cultures of sweet orange (Citrus sinensis L. Osbeck). Environmental and Experimental Botany 35, 455-463, 1995.-Activities of the enzymes involved in sucrose metabolism were investigated in embryogenic nucellus-derived calli of "Mamlin" sweet orange, Citrus sinensis L. Osbeck, cultured in suspension medium containing glycerol or surcose as the only carbon sources. Activities of sucrose phosphate synthase (SPS) and sucrose (SS), determined after 14 days of culture, were about 2.5-fold higher in the 2% glycerol-grown tissues, as compared to the 5% sucrose-grown tissues. Futhermore,activity of SPS was about two-fold and three-fold higher in the glycerol- and sucrose-grown tissues, respectively, when 40 mM glucose 6-posphate (G6P) was added to the assay medium containing uridine 5' -diphosphoglucose (UDPG) and fructose 6-phosphate. SPS activity increased 43% for the glycerol-grown tissues and 130% for the sucrose-grown tissues when only UDPG and G6P were present in the assay reaction. Gel electrophoretic analysis and catalytic determination confirmed the presence of phosphoglucose isomeras in the tissues of both glycerol-and sucrose-cotaining medium. |
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Palavras-Chave: |
Cultura de tecidos; Enzimas; Sucrose metabolism enzymes. |
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Thesagro: |
Análise; Carboidrato; Laranja. |
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Thesaurus Nal: |
Citrus; glycerol; tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02001naa a2200253 a 4500
001 1634239
005 1996-02-02
008 1995 bl uuuu u00u1 u #d
100 1 $aVU, J. C. V.
245 $aActivities of sucrose metabolism enzymes in glycerol-grown suspension cultures of sweet orange (Citrus Sinensis L. Osbeck).
260 $c1995
520 $aVu J.C.V., Niedz R.P. and Yelenosky G. Activities of sucrose metabolism enzymes in glycerol-grown suspension cultures of sweet orange (Citrus sinensis L. Osbeck). Environmental and Experimental Botany 35, 455-463, 1995.-Activities of the enzymes involved in sucrose metabolism were investigated in embryogenic nucellus-derived calli of "Mamlin" sweet orange, Citrus sinensis L. Osbeck, cultured in suspension medium containing glycerol or surcose as the only carbon sources. Activities of sucrose phosphate synthase (SPS) and sucrose (SS), determined after 14 days of culture, were about 2.5-fold higher in the 2% glycerol-grown tissues, as compared to the 5% sucrose-grown tissues. Futhermore,activity of SPS was about two-fold and three-fold higher in the glycerol- and sucrose-grown tissues, respectively, when 40 mM glucose 6-posphate (G6P) was added to the assay medium containing uridine 5' -diphosphoglucose (UDPG) and fructose 6-phosphate. SPS activity increased 43% for the glycerol-grown tissues and 130% for the sucrose-grown tissues when only UDPG and G6P were present in the assay reaction. Gel electrophoretic analysis and catalytic determination confirmed the presence of phosphoglucose isomeras in the tissues of both glycerol-and sucrose-cotaining medium.
650 $aCitrus
650 $aglycerol
650 $atissue culture
650 $aAnálise
650 $aCarboidrato
650 $aLaranja
653 $aCultura de tecidos
653 $aEnzimas
653 $aSucrose metabolism enzymes
700 1 $aNIEDZ, R. P.
700 1 $aYELENOSKY, G.
773 $tEnvironmental and experimental botany$gv. 35, n. 4, 455-463, 1995
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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