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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos; Embrapa Instrumentação. |
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Data corrente: |
03/10/2011 |
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Data da última atualização: |
29/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FARINAS, C. S.; VITCOSQUE, G. L.; FONSECA, R. F.; BERTUCCI NETO, V.; COURI, S. |
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Afiliação: |
CRISTIANE SANCHEZ FARINAS, CNPDIA; VICTOR BERTUCCI NETO, CNPDIA. |
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Título: |
Modeling the effects of solid state fermentation operating conditions on endoglucanase production using an instrumented bioreactor. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Industrial crops and products, [S. l.], v. 34, n. 1, p. 1186-1192, 2011. |
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ISSN: |
0926-6690 |
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DOI: |
10.1016/j.indcrop.2011.04.006 |
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Idioma: |
Inglês |
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Conteúdo: |
Cellulase production is one of the most critical steps in the economics of second generation ethanol. Although solid-state fermentation (SSF) is an attractive process for the production of enzymes, SSF is highly limited by the difficulty in controlling the operating variables which affect microbial growth and metabolites production. In this context, this work evaluates the effects of operational conditions on endoglucanase production by a selected strain of Aspergillus niger cultivated under SSF using an instrumented lab-scale bioreactor equipped with an on-line automated monitoring and control system. The effects of air flow rate, inlet air relative humidity and substrate initial moisture on endoglucanase production were evaluated using a statistical design methodology. A correlation coefficient of 0.9106 and a calculated value of F, 5.46 folds higher than the listed value (P-value < 0.05) allowed the modeling of endoglucanase production under different process conditions. Higher endoglucanase production (56.1 U/g) was achieved for a selected condition of substrate initial moisture of 72%, air inlet humidity of 70%, and flow rate of 20 mL/min. A significant increase in endoglucanase production was also found to be achieved under forced aeration conditions (50.2 IU/g) compared to static conditions (29.8 IU/g) after 72 h of cultivation. Besides, respirometric analysis revealed that the total amount of CO2 produced was linearly correlated with enzyme production (R2 of 0.988). The bioreactor system used, as well as the methodology employed herein, was very effective in evaluating the influence of operational variables on enzymes production under SSF. MenosCellulase production is one of the most critical steps in the economics of second generation ethanol. Although solid-state fermentation (SSF) is an attractive process for the production of enzymes, SSF is highly limited by the difficulty in controlling the operating variables which affect microbial growth and metabolites production. In this context, this work evaluates the effects of operational conditions on endoglucanase production by a selected strain of Aspergillus niger cultivated under SSF using an instrumented lab-scale bioreactor equipped with an on-line automated monitoring and control system. The effects of air flow rate, inlet air relative humidity and substrate initial moisture on endoglucanase production were evaluated using a statistical design methodology. A correlation coefficient of 0.9106 and a calculated value of F, 5.46 folds higher than the listed value (P-value < 0.05) allowed the modeling of endoglucanase production under different process conditions. Higher endoglucanase production (56.1 U/g) was achieved for a selected condition of substrate initial moisture of 72%, air inlet humidity of 70%, and flow rate of 20 mL/min. A significant increase in endoglucanase production was also found to be achieved under forced aeration conditions (50.2 IU/g) compared to static conditions (29.8 IU/g) after 72 h of cultivation. Besides, respirometric analysis revealed that the total amount of CO2 produced was linearly correlated with enzyme production (R2 of 0.988). ... Mostrar Tudo |
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Palavras-Chave: |
Bioetanol; Bioreactor; Biorreator; Cellulase; Endoglucanase; Enzimas; Fermentação em estado sólido; Solid-state fermentation. |
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Thesagro: |
Celulase; Enzima; Fermentação. |
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Thesaurus Nal: |
Bioethanol; Enzymes. |
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Categoria do assunto: |
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X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02684naa a2200349 a 4500
001 1901985
005 2019-01-29
008 2011 bl uuuu u00u1 u #d
022 $a0926-6690
024 7 $a10.1016/j.indcrop.2011.04.006$2DOI
100 1 $aFARINAS, C. S.
245 $aModeling the effects of solid state fermentation operating conditions on endoglucanase production using an instrumented bioreactor.
260 $c2011
520 $aCellulase production is one of the most critical steps in the economics of second generation ethanol. Although solid-state fermentation (SSF) is an attractive process for the production of enzymes, SSF is highly limited by the difficulty in controlling the operating variables which affect microbial growth and metabolites production. In this context, this work evaluates the effects of operational conditions on endoglucanase production by a selected strain of Aspergillus niger cultivated under SSF using an instrumented lab-scale bioreactor equipped with an on-line automated monitoring and control system. The effects of air flow rate, inlet air relative humidity and substrate initial moisture on endoglucanase production were evaluated using a statistical design methodology. A correlation coefficient of 0.9106 and a calculated value of F, 5.46 folds higher than the listed value (P-value < 0.05) allowed the modeling of endoglucanase production under different process conditions. Higher endoglucanase production (56.1 U/g) was achieved for a selected condition of substrate initial moisture of 72%, air inlet humidity of 70%, and flow rate of 20 mL/min. A significant increase in endoglucanase production was also found to be achieved under forced aeration conditions (50.2 IU/g) compared to static conditions (29.8 IU/g) after 72 h of cultivation. Besides, respirometric analysis revealed that the total amount of CO2 produced was linearly correlated with enzyme production (R2 of 0.988). The bioreactor system used, as well as the methodology employed herein, was very effective in evaluating the influence of operational variables on enzymes production under SSF.
650 $aBioethanol
650 $aEnzymes
650 $aCelulase
650 $aEnzima
650 $aFermentação
653 $aBioetanol
653 $aBioreactor
653 $aBiorreator
653 $aCellulase
653 $aEndoglucanase
653 $aEnzimas
653 $aFermentação em estado sólido
653 $aSolid-state fermentation
700 1 $aVITCOSQUE, G. L.
700 1 $aFONSECA, R. F.
700 1 $aBERTUCCI NETO, V.
700 1 $aCOURI, S.
773 $tIndustrial crops and products, [S. l.]$gv. 34, n. 1, p. 1186-1192, 2011.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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| Registros recuperados : 10 | |
| 2. |  | VITCOSQUE, G. L.; FARINAS, C. S.; FONSECA, R. F.; BERTUCCI NETO, V. Improving cellulolytic enzyme production using sugarcane bagasse in a solid-state fermentation bioreactor. Holos environment, Rio Claro, v. 11, n. 1, p. 103, 2011. Supl. 1. Edição dos resumos do 5 Simpósio de Microbiologia Aplicada, Rio Claro, maio 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 7. | | FARINAS, C. S.; VITCOSQUE, G. L.; FONSECA, R. F.; BERTUCCI NETO, V.; COURI, S. Modeling the effects of solid state fermentation operating conditions on endoglucanase production using an instrumented bioreactor. Industrial crops and products, [S. l.], v. 34, n. 1, p. 1186-1192, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Instrumentação. |
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| 9. | | VITCOSQUE, G. L.; FONSECA, R. F.; RODRÍGUEZ-ZUÑIGA, U. F.; BERTUCCI-NETO, V.; COURI, S.; FARINAS, C. S. Use of instrumented bioreactor for the production of biomass-degrading multienzymes under solid state fermentation of soybean meal. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 34., 2012, New Orleans. Abstract... Old Lee Highway: Society for Industrial Microbiology & Biotechnology, 2012. Paper 20758.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 10. | | VITCOSQUE, G. L.; RIBEIRO, L. F. C.; LUCAS, R. C. de; SILVA, T. M. da; RIBEIRO, L. F.; DAMASIO, A. R. de L.; FARINAS, C. S.; GONCALVES, A. Z. L.; SEGATO, F.; BUCKERIDGE, M. S.; JORGE, J. A.; POLIZELI, M. de L. T. M. The functional properties of a xyloglucanase (GH12) of Aspergillus terreus expressed in Aspergillus nidulans may increase performance of biomass degradation. Applied microbiology and biotechnology, [S. ], v. 100, n. 21, p. 9133-9144, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| Registros recuperados : 10 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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