Data/hora: 17/06/2024 01:03:52

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 31/03/2014
Data da última atualização: | 26/05/2014
Autoria: | MASCARIN, G. M.; KOBORI, N. N.; VITAL, R. C. de J.; JACKSON, M. A.; QUINTELA, E. D.
Afiliação: | GABRIEL MOURA MASCARIN, CNPAF; NILCE NAOMI KOBORI; RAYAN CARLOS DE JESUS VITAL; MARK ALAN JACKSON, USDA; ELIANE DIAS QUINTELA, CNPAF.
Título: | Production of microsclerotia by Brazilian strains of Metarhizium spp. using submerged liquid culture fermentation.
Ano de publicação: | 2014
Fonte/Imprenta: | World Journal of Microbiology & Biotechnology, Oxford, v. 30, n. 5, p. 1583-1590, May 2014.
DOI: | DOI 10.1007/s11274-013-1581-0
Idioma: | Inglês
Conteúdo: | We investigated the potential production and desiccation tolerance of microsclerotia (MS) by Brazilian strains of Metarhizium anisopliae (Ma), M. acridum (Mc) and M. robertsii (Mr). These fungi were grown in a liquid medium containing 16 g carbon l-1 with a carbon:nitrogen ratio of 50:1. One hundred milliliters cultures were grown in 250 ml Erlenmeyer flasks in a rotary incubator shaker at 28 C and 200 rpm for 5 days. Five-day-old MS were harvested, mixed with diatomaceous earth (DE) and airdried for 2 days at 30 C. The air-dried MS?DE granular preparations were milled by mortar ? pestle and stored in centrifuged tubes at either 26 or -20 C. Desiccation tolerance and conidia production were assessed for dried MS granules by measuring hyphal germination after incubation for 2 days on water agar plates at 26 C and for conidia production following 7 days incubation. Yields of MS by all strains of Metarhizium were 6.1?7.3 9 106 l-1 after 3 days growth with maximum MS yields (0.7?1.1 9 107 l-1) after 5 days growth. No differences in biomass accumulation were observed after 3 days growth, whereas Ma- CG168 showed the highest biomass accumulation after 5 days growth. Dried MS?DE preparations of all fungal strains were equally tolerant to desiccation (C93 % germination) and the highest conidia production was obtained by MS granules of Mc-CG423 (4 9 109 conidia g-1). All MS granules showed similar stability after storage at either 26 or -20 C for 3.5 months.
Thesagro: | Controle biológico; Metarhizium anisopliae.
Thesaurus Nal: | Ascomycota; Biological control; Drying; Mycoinsecticides.
Categoria do assunto: | O Insetos e Entomologia
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (32553-1) - UPC - AP - DD - 2014.008 -  - 2014.008 - DIS