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Registro Completo |
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
14/10/2015 |
Data da última atualização: |
18/02/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GAVA, D.; SOUZA, C. K.; SCHAEFER, R.; VINCENT, A. L.; CANTAO, M. E.; COLDEBELLA, A.; ZANELLA, J. R. C. |
Afiliação: |
DANIELLE GAVA, CNPSA; CARINE KUNZLER DE SOUZA, UFRGS; REJANE SCHAEFER, CNPSA; AMY LOUISE VINCENT, USDA/ARS; MAURICIO EGIDIO CANTAO, CNPSA; ARLEI COLDEBELLA, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA. |
Título: |
A TaqMan-based real-time PCR for detection and quantification of porcine parvovirus 4. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Journal of Virological Methods, v. 219, p. 14-17, 2015. |
DOI: |
http://dx.doi.org/10.1016/j.jviromet.2015.03.011 |
Idioma: |
Inglês |
Conteúdo: |
Porcine parvovirus 4 (PPV4) is a DNA virus, and a member of the Parvoviridae family within the Bocavirus genera. It was detected recently in swine, but its epidemiology and pathology remain unclear. A TaqManbased real-time PCR (qPCR) targeting a conserved region of the ORF3 gene of PPV4 was developed. The qPCR detection limit was 9.5 × 101 DNA copies/L. There was no cross-reaction with porcine parvovirus, torque teno virus 1, torque teno virus 2, porcine circovirus type 1, porcine circovirus type 2, or with pseudorabies virus. Two hundred and seventy-two samples, including serum, semen, lungs, feces, ovarian follicular fluids, ovaries and uterus, were evaluated by qPCR and PPV4 was detected in 36 samples (13.2%). When compared with a conventional PCR (cPCR), the qPCR assay was 10 times more sensitive and the detection of PPV4 DNA in field samples was increased 2.5 times. Partial sequencing of PPV4 ORF3 gene, obtained from two pooled samples of uterus and ovaries, revealed a high nucleotide identity (98?99%) with a reference PPV4 sequence. The qPCR can be used as a fast and accurate assay for the detection and quantification of PPV4 in field samples and for epidemiological studies in swine herds. |
Palavras-Chave: |
Porcine parvovirus; Swine disease. |
Thesagro: |
Doença animal; Parvovirose; Suíno; Virologia. |
Thesaurus Nal: |
Parvoviridae; Quantitative polymerase chain reaction; SsDNA viruses; Virology. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02164naa a2200325 a 4500 001 2026395 005 2016-02-18 008 2015 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.1016/j.jviromet.2015.03.011$2DOI 100 1 $aGAVA, D. 245 $aA TaqMan-based real-time PCR for detection and quantification of porcine parvovirus 4.$h[electronic resource] 260 $c2015 520 $aPorcine parvovirus 4 (PPV4) is a DNA virus, and a member of the Parvoviridae family within the Bocavirus genera. It was detected recently in swine, but its epidemiology and pathology remain unclear. A TaqManbased real-time PCR (qPCR) targeting a conserved region of the ORF3 gene of PPV4 was developed. The qPCR detection limit was 9.5 × 101 DNA copies/L. There was no cross-reaction with porcine parvovirus, torque teno virus 1, torque teno virus 2, porcine circovirus type 1, porcine circovirus type 2, or with pseudorabies virus. Two hundred and seventy-two samples, including serum, semen, lungs, feces, ovarian follicular fluids, ovaries and uterus, were evaluated by qPCR and PPV4 was detected in 36 samples (13.2%). When compared with a conventional PCR (cPCR), the qPCR assay was 10 times more sensitive and the detection of PPV4 DNA in field samples was increased 2.5 times. Partial sequencing of PPV4 ORF3 gene, obtained from two pooled samples of uterus and ovaries, revealed a high nucleotide identity (98?99%) with a reference PPV4 sequence. The qPCR can be used as a fast and accurate assay for the detection and quantification of PPV4 in field samples and for epidemiological studies in swine herds. 650 $aParvoviridae 650 $aQuantitative polymerase chain reaction 650 $aSsDNA viruses 650 $aVirology 650 $aDoença animal 650 $aParvovirose 650 $aSuíno 650 $aVirologia 653 $aPorcine parvovirus 653 $aSwine disease 700 1 $aSOUZA, C. K. 700 1 $aSCHAEFER, R. 700 1 $aVINCENT, A. L. 700 1 $aCANTAO, M. E. 700 1 $aCOLDEBELLA, A. 700 1 $aZANELLA, J. R. C. 773 $tJournal of Virological Methods$gv. 219, p. 14-17, 2015.
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Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Amazônia Ocidental. |
Data corrente: |
26/07/2000 |
Data da última atualização: |
29/05/2017 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
SÁ SOBRINHO, A. F. de; GARCIA, L. C. |
Título: |
Alfavaca (Ocimum basilicum L.). |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
In: CARDOSO, M. O.(Org.). Hortaliças não-convencionais da Amazônia. Brasília, DF: EMBRAPA-SPI; Manaus: EMBRAPA-CPAA, 1997. p. 115-119. |
Idioma: |
Português |
Conteúdo: |
Aspectos gerais; Caracteristicas botanicas e variedades; Exigencias de clima e solos; Propagacao e cultivo; Pragas e doencas; Colheita e comercializacao. |
Palavras-Chave: |
Amazonas; Brasil; Cochonilhas; Cultivar; Cultivation; Cultivo; Diseases; Harvest; Hortalica alternativa; Nematodes; Pest insects; Postharvest; Propagacao; Propagation. |
Thesagro: |
Alfavaca; Botânica; Clima; Colheita; Comercialização; Doença; Inseto; Meloidogyne Incognita; Nematóide; Ocimum Basilicum; Pós-Colheita; Praga; Pulgão; Solo; Variedade. |
Thesaurus NAL: |
botany; Brazil; climate; marketing; soil; varieties. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/123212/1/p.-115-119-1.pdf
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Marc: |
LEADER 01506naa a2200553 a 4500 001 2014762 005 2017-05-29 008 1997 bl uuuu u00u1 u #d 100 1 $aSÁ SOBRINHO, A. F. de 245 $aAlfavaca (Ocimum basilicum L.). 260 $c1997 520 $aAspectos gerais; Caracteristicas botanicas e variedades; Exigencias de clima e solos; Propagacao e cultivo; Pragas e doencas; Colheita e comercializacao. 650 $abotany 650 $aBrazil 650 $aclimate 650 $amarketing 650 $asoil 650 $avarieties 650 $aAlfavaca 650 $aBotânica 650 $aClima 650 $aColheita 650 $aComercialização 650 $aDoença 650 $aInseto 650 $aMeloidogyne Incognita 650 $aNematóide 650 $aOcimum Basilicum 650 $aPós-Colheita 650 $aPraga 650 $aPulgão 650 $aSolo 650 $aVariedade 653 $aAmazonas 653 $aBrasil 653 $aCochonilhas 653 $aCultivar 653 $aCultivation 653 $aCultivo 653 $aDiseases 653 $aHarvest 653 $aHortalica alternativa 653 $aNematodes 653 $aPest insects 653 $aPostharvest 653 $aPropagacao 653 $aPropagation 700 1 $aGARCIA, L. C. 773 $tIn: CARDOSO, M. O.(Org.). Hortaliças não-convencionais da Amazônia. Brasília, DF: EMBRAPA-SPI; Manaus: EMBRAPA-CPAA, 1997. p. 115-119.
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