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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
05/08/2020 |
Data da última atualização: |
10/08/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MALARD, P. F.; PEIXER, M. A. S.; GRAZIA, J. G.; BRUNEL, H. dos S. S.; FERES, L. F.; VILLARROEL, C. L.; SIQUEIRA, L. G. B.; DODE, M. A. N.; POGUE, R.; VIANA, J. H. M.; CARVALHO, J. L. |
Afiliação: |
PATRICIA F. MALARD, UCB; MAURICIO A. S. PEIXER, UCB; JOAO G. GRAZIA, CENATTE EMBRIÕES; HILANA DOS SANTOS SENA BRUNEL, BIO BIOTECNOLOGIA DA REPRODUÇÃO ANIMAL; LUIZ F. FERES, UNIFAL; CARLA L. VILLARROEL, BIO BIOTECNOLOGIA DA REPRODUÇÃO ANIMAL; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MARGOT ALVES NUNES DODE, Cenargen; ROBERT POGUE, UCB; JOAO HENRIQUE MOREIRA VIANA, Cenargen; JULIANA L. CARVALHO, UCB. |
Título: |
Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Scientific Reports, v. 10, article 8018, 2020. |
DOI: |
https://doi.org/10.1038/s41598-020-64810-x |
Idioma: |
Inglês |
Conteúdo: |
Valuable female cattle are continuously subject to follicular puncture (ovum pick-up - OPU). This technique is commonly used for in-vitro embryo production, but may result in ovarian lesion. Mesenchymal stem cells (MSC) ameliorate the function of injured tissues, but their use to treat ovarian lesions in cattle has not been established. We investigated whether a local injection of MSC would reduce the negative efects of repeated OPU under acute and chronic scenarios in bovines. First, we performed four OPU sessions and injected 2.5×106 MSCs immediately after the 4th OPU procedure (n=5). The treated organs (right ovary) were compared to their saline-treated counterparts (left), and presented superior production of oocytes and embryos in the three following OPU sessions (P<0.05). Then, cows with progressive fertility loss went through three OPU sessions. Animals received MSC, saline, or MSC+FSH in both ovaries after the frst OPU. In the two following OPU sessions, the MSC and MSC +FSH - treated groups failed to present any signifcant alteration in the number of oocytes and embryos compared to saline-treated animals. Thus, MSC have benefcial efects on the fertility of OPUlesioned cows, but not in cows with cystic ovarian disease and chronic ovarian lesions. |
Palavras-Chave: |
Fertility; Fertilização in-vitro; Mesenchymal stem cells; MSC; OPU; Ovum pick-up. |
Thesagro: |
Biotecnologia; Bovino; Fertilidade Animal. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/224978/1/Intraovarian-injection.pdf
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Marc: |
LEADER 02359naa a2200361 a 4500 001 2133327 005 2021-08-10 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1038/s41598-020-64810-x$2DOI 100 1 $aMALARD, P. F. 245 $aIntraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss.$h[electronic resource] 260 $c2020 520 $aValuable female cattle are continuously subject to follicular puncture (ovum pick-up - OPU). This technique is commonly used for in-vitro embryo production, but may result in ovarian lesion. Mesenchymal stem cells (MSC) ameliorate the function of injured tissues, but their use to treat ovarian lesions in cattle has not been established. We investigated whether a local injection of MSC would reduce the negative efects of repeated OPU under acute and chronic scenarios in bovines. First, we performed four OPU sessions and injected 2.5×106 MSCs immediately after the 4th OPU procedure (n=5). The treated organs (right ovary) were compared to their saline-treated counterparts (left), and presented superior production of oocytes and embryos in the three following OPU sessions (P<0.05). Then, cows with progressive fertility loss went through three OPU sessions. Animals received MSC, saline, or MSC+FSH in both ovaries after the frst OPU. In the two following OPU sessions, the MSC and MSC +FSH - treated groups failed to present any signifcant alteration in the number of oocytes and embryos compared to saline-treated animals. Thus, MSC have benefcial efects on the fertility of OPUlesioned cows, but not in cows with cystic ovarian disease and chronic ovarian lesions. 650 $aBiotecnologia 650 $aBovino 650 $aFertilidade Animal 653 $aFertility 653 $aFertilização in-vitro 653 $aMesenchymal stem cells 653 $aMSC 653 $aOPU 653 $aOvum pick-up 700 1 $aPEIXER, M. A. S. 700 1 $aGRAZIA, J. G. 700 1 $aBRUNEL, H. dos S. S. 700 1 $aFERES, L. F. 700 1 $aVILLARROEL, C. L. 700 1 $aSIQUEIRA, L. G. B. 700 1 $aDODE, M. A. N. 700 1 $aPOGUE, R. 700 1 $aVIANA, J. H. M. 700 1 $aCARVALHO, J. L. 773 $tScientific Reports$gv. 10, article 8018, 2020.
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Embrapa Gado de Leite (CNPGL) |
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1. | | MALARD, P. F.; PEIXER, M. A. S.; GRAZIA, J. G.; BRUNEL, H. dos S. S.; FERES, L. F.; VILLARROEL, C. L.; SIQUEIRA, L. G. B.; DODE, M. A. N.; POGUE, R.; VIANA, J. H. M.; CARVALHO, J. L. Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. Scientific Reports, v. 10, article 8018, 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 1 | |
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