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Registro Completo |
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
28/01/2009 |
Data da última atualização: |
28/01/2009 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
VILLALOBOS, A. A. C.; SEVERINO, L. S.; SABEL, J. M. del A. |
Afiliação: |
Alina A. Camacho Villalobos, INIA; Liv Soares Severino, Embrapa Algodão; Juan Marcel del Águila Sabel, DEVIDA. |
Título: |
Adaptación de tecnologia de fertilización en un cultivar de higuerilla (Ricinus communis L.) introducido de Brasil para la región Ucayali en la selva del Perú 2007-2008. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MAMONA, 3., 2008, Salvador. Energia e ricinoquímica: anais. Salvador: SEAGRI: Embrapa Algodão, 2008. |
Descrição Física: |
1 CD-ROM |
Idioma: |
Português |
Palavras-Chave: |
Adaptação da mamona; Fertilização da mamona. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPA-2009-09/21883/1/FA-08.pdf
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Marc: |
LEADER 00667nam a2200157 a 4500 001 1277305 005 2009-01-28 008 2008 bl uuuu u00u1 u #d 100 1 $aVILLALOBOS, A. A. C. 245 $aAdaptación de tecnologia de fertilización en un cultivar de higuerilla (Ricinus communis L.) introducido de Brasil para la región Ucayali en la selva del Perú 2007-2008. 260 $aIn: CONGRESSO BRASILEIRO DE MAMONA, 3., 2008, Salvador. Energia e ricinoquímica: anais. Salvador: SEAGRI: Embrapa Algodão$c2008 300 $c1 CD-ROM 653 $aAdaptação da mamona 653 $aFertilização da mamona 700 1 $aSEVERINO, L. S. 700 1 $aSABEL, J. M. del A.
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Embrapa Algodão (CNPA) |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
27/07/2005 |
Data da última atualização: |
14/08/2024 |
Autoria: |
INGLIS, P. W.; RIGDEN, D. J.; MELLO, L. V.; LOUIS, E. J.; VALADARES-INGLIS, M. C. |
Título: |
Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae, contains a RecQ helicase-like gene. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
Molecular Genetics and Genomics, v. 274, p. 79-90, 2005. |
Idioma: |
Inglês |
Notas: |
Published online: 2 June 2005. |
Conteúdo: |
In most filamentous fungi, telomere-associated sequences (TASs) are polymorphic, and the presence of restriction fragment length polymorphisms (RFLPs) may permit the number of chromosome ends to be estimated from the number of telomeric bands obtained by restriction digestion. Here, we describe strains of Metarhizium, Gliocladium and Paecilomyces species in which only one or a few telomeric bands of unequal intensity are detectable by Southern hybridization, indicating that interchromosomal TAS exchange occurs. We also studied an anomalous strain of Metarhizium anisopliae, which produces polymorphic telomeric bands larger than 8 kb upon digestion of genomic DNA with XhoI. In this case, the first XhoI site in from the chromosome end must lie beyond the presumed monomorphic region. Cloned telomeres from this strain comprise 18?26 TTAGGG repeats, followed at the internal end of the telomere tract by five repeats of the telomere-like sequence TAAACGCTGG. An 8.1-kb TAS clone also contains a gene for a RecQ-like helicase, designated TAH1, suggesting that this TAS is analogous to the Y elements in yeast and the subtelomeric helicase ORFs of Ustilago maydis (UTASRecQ) and Magnaporthe grisea (TLH1). The TAS in the anomalous strain of M. anisopliae, however, appears distinct from these in that it is found at most telomeres and its predicted protein product possesses a significantly longer N-terminal region in comparison to the M. grisea and U. maydis helicases. Hybridization analyses showed that TAH1 homologues are present in all other anomalous M. anisopliae strains studied, as well as in some other polymorphic strains, where the recQ-like gene also appears to be telomere-associated. MenosIn most filamentous fungi, telomere-associated sequences (TASs) are polymorphic, and the presence of restriction fragment length polymorphisms (RFLPs) may permit the number of chromosome ends to be estimated from the number of telomeric bands obtained by restriction digestion. Here, we describe strains of Metarhizium, Gliocladium and Paecilomyces species in which only one or a few telomeric bands of unequal intensity are detectable by Southern hybridization, indicating that interchromosomal TAS exchange occurs. We also studied an anomalous strain of Metarhizium anisopliae, which produces polymorphic telomeric bands larger than 8 kb upon digestion of genomic DNA with XhoI. In this case, the first XhoI site in from the chromosome end must lie beyond the presumed monomorphic region. Cloned telomeres from this strain comprise 18?26 TTAGGG repeats, followed at the internal end of the telomere tract by five repeats of the telomere-like sequence TAAACGCTGG. An 8.1-kb TAS clone also contains a gene for a RecQ-like helicase, designated TAH1, suggesting that this TAS is analogous to the Y elements in yeast and the subtelomeric helicase ORFs of Ustilago maydis (UTASRecQ) and Magnaporthe grisea (TLH1). The TAS in the anomalous strain of M. anisopliae, however, appears distinct from these in that it is found at most telomeres and its predicted protein product possesses a significantly longer N-terminal region in comparison to the M. grisea and U. maydis helicases. Hybridization analyses ... Mostrar Tudo |
Palavras-Chave: |
Recombination; RecQ helicase; Telomere associated sequence. |
Thesagro: |
Fungo; Metarhizium Anisopliae. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178387/1/ID-253071.pdf
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Marc: |
LEADER 02462naa a2200241 a 4500 001 1185898 005 2024-08-14 008 2005 bl uuuu u00u1 u #d 100 1 $aINGLIS, P. W. 245 $aMonomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae, contains a RecQ helicase-like gene.$h[electronic resource] 260 $c2005 500 $aPublished online: 2 June 2005. 520 $aIn most filamentous fungi, telomere-associated sequences (TASs) are polymorphic, and the presence of restriction fragment length polymorphisms (RFLPs) may permit the number of chromosome ends to be estimated from the number of telomeric bands obtained by restriction digestion. Here, we describe strains of Metarhizium, Gliocladium and Paecilomyces species in which only one or a few telomeric bands of unequal intensity are detectable by Southern hybridization, indicating that interchromosomal TAS exchange occurs. We also studied an anomalous strain of Metarhizium anisopliae, which produces polymorphic telomeric bands larger than 8 kb upon digestion of genomic DNA with XhoI. In this case, the first XhoI site in from the chromosome end must lie beyond the presumed monomorphic region. Cloned telomeres from this strain comprise 18?26 TTAGGG repeats, followed at the internal end of the telomere tract by five repeats of the telomere-like sequence TAAACGCTGG. An 8.1-kb TAS clone also contains a gene for a RecQ-like helicase, designated TAH1, suggesting that this TAS is analogous to the Y elements in yeast and the subtelomeric helicase ORFs of Ustilago maydis (UTASRecQ) and Magnaporthe grisea (TLH1). The TAS in the anomalous strain of M. anisopliae, however, appears distinct from these in that it is found at most telomeres and its predicted protein product possesses a significantly longer N-terminal region in comparison to the M. grisea and U. maydis helicases. Hybridization analyses showed that TAH1 homologues are present in all other anomalous M. anisopliae strains studied, as well as in some other polymorphic strains, where the recQ-like gene also appears to be telomere-associated. 650 $aFungo 650 $aMetarhizium Anisopliae 653 $aRecombination 653 $aRecQ helicase 653 $aTelomere associated sequence 700 1 $aRIGDEN, D. J. 700 1 $aMELLO, L. V. 700 1 $aLOUIS, E. J. 700 1 $aVALADARES-INGLIS, M. C. 773 $tMolecular Genetics and Genomics$gv. 274, p. 79-90, 2005.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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