Data/hora: 19/06/2024 01:45:31

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 26/11/1999
Data da última atualização: | 15/05/2023
Autoria: | DORNELAS, M. C.; TAVARES, F. C. A.; OLIVEIRA, J. C. de; VIEIRA, M. L. C.
Afiliação: | Departmento de Genetica, Escola Superior de Agricultura "Luiz de Queiroz", Uniniversidade de Sao Paulo, P.O. Box 83, Piracicaba 13418-900, Brasil.
Título: | Plant regeneration from protoplast fusion in Passiflora spp.
Ano de publicação: | 1995
Fonte/Imprenta: | Plant Cell Reports, v.15, p.106-110, 1995.
Idioma: | Inglês
Conteúdo: | Protoplasts were isolated from leaf explants of Passiflora edulis var. flavicarpa (the yellow passion fruit) and from cell suspensions of five Passiflora species. Chemical fusion was performed using polyethylene glycol and the microcolonies obtained were transferred to growth medium to produce calli. Electrophoresis of soluble proteins and analysis of isoenzymes from calli produced from the fusion experiments were performed to select somatic hybrids. Specific polypeptide bands allowed the identification of somatic hybrids between P. edulis var. flavicarpa (+) P. alata, P. edulis var. flavicarpa (+) P. amethystina, P. edulis var. flavicarpa (+) P. cincinnata, P. edulis var. flavicarpa (+) P. giberti and P. edulis var. flavicarpa (+) P. coccinea. An average of 3 to 5% hybrid calli were obtained.
Palavras-Chave: | Cultura de tecidos; Fusao de protoplasto; Hibridacao somatica; Passionfruit.
Thesagro: | Maracujá.
Thesaurus Nal: | Passiflora; protoplast fusion; somatic hybridization; tissue culture.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (17460-1) - ADD - AP - DD - Publicação digital -  -  - DIS