Data/hora: 16/06/2024 07:17:19

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	13/11/2015	
Data da última atualização: 	18/05/2023	
Autoria: 	SOUZA, F. V. D.; KAYA, E.; VIEIRA, L. de J.; SOUZA, E. H. de; AMORIM, V. B. de O.; SKOGERBOE, D.; MATSUMOTO, T.; ALVES, A. A. C.; LEDO, C. A. da S.; JENDEREK, M. M.	
Afiliação: 	FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; ERGUN KAYA, National Center for Genetic Resources Preservation; LÍVIA DE JESUS VIEIRA; EVERTON HILO DE SOUZA; VANUSIA BATISTA DE OLIVEIRA AMORIM; DIANNE SKOGERBOE, National Center for Genetic Resources Preservation; TRACIE MATSUMOTO, Pacific Basin Agriculture Research Center; ALFREDO AUGUSTO CUNHA ALVES, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; MARIA M. JENDEREK, National Center for Genetic Resources Preservation.	
Título: 	Droplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes.	
Ano de publicação: 	2015	
Fonte/Imprenta: 	Plant Cell, Tissue and Organ Culture, v.123, n.2, nov. 2015.	
ISSN: 	0167-6857	
DOI: 	10.1007/s11240-015-0899-8	
Idioma: 	Português	
Conteúdo: 	Germplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the first report on histological changes occurring in cryopreserved Ananas tissue.	
Thesagro: 	Abacaxi; Ananás Comosus.	
Thesaurus Nal: 	Histology; Pineapples.	
Categoria do assunto: 	G Melhoramento Genético	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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