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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/10/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIDAL, Á. M.; VIEIRA, L. J.; FERREIRA, C. F.; SOUZA, F. V. D.; SOUZA, A. da S.; LEDO, C. A. da S. |
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Afiliação: |
Á. M. VIDAL, UFRB; L. J. VIEIRA; CLAUDIA FORTES FERREIRA, CNPMF; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF. |
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Título: |
Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v.14, n. 3, p. 7759-7770, 2015. |
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ISSN: |
1676-5680 |
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Idioma: |
Inglês |
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Conteúdo: |
Molecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic complement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava plants derived from meristem culture, demonstrating the reliability of this propagation system. MenosMolecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic complement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava p... Mostrar Tudo |
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Palavras-Chave: |
Marcado molecular. |
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Thesagro: |
Anatomia vegetal; Banco de germoplasma; Mandioca; Meristema. |
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Thesaurus Nal: |
Cassava; Genetic markers; Germplasm. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02450naa a2200289 a 4500
001 2027402
005 2023-05-18
008 2015 bl uuuu u00u1 u #d
022 $a1676-5680
100 1 $aVIDAL, Á. M.
245 $aGenetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers.$h[electronic resource]
260 $c2015
520 $aMolecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic complement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava plants derived from meristem culture, demonstrating the reliability of this propagation system.
650 $aCassava
650 $aGenetic markers
650 $aGermplasm
650 $aAnatomia vegetal
650 $aBanco de germoplasma
650 $aMandioca
650 $aMeristema
653 $aMarcado molecular
700 1 $aVIEIRA, L. J.
700 1 $aFERREIRA, C. F.
700 1 $aSOUZA, F. V. D.
700 1 $aSOUZA, A. da S.
700 1 $aLEDO, C. A. da S.
773 $tGenetics and Molecular Research$gv.14, n. 3, p. 7759-7770, 2015.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
11/08/2009 |
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Data da última atualização: |
11/08/2009 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
CHAVES, F. C. M.; MAGALHÃES, P. M. de; HIDALGO, A. de F.; POHLIT, A. M. |
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Afiliação: |
Francisco Celio Maia Chaves, CPAA; Pedro Melillo de Magalhães, CPQBA/Unicamp; Ari de Freitas Hidalgo, UFAM; Adrian Martin Pohlit, Inpa. |
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Título: |
Produção de biomassa de Artemisia annua L. em três ecossistemas amazônicos. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE OLERICULTURA, 49., 2009, Águas de Lindóia. Água na horticultura: novas atitudes e uso sustentável. Horticultura Brasileira, v. 27, n. 2, S2564-S2569, ago. 2009. Suplemento. |
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Idioma: |
Português |
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Conteúdo: |
Avaliou-se a produção de Artemisia annua em três ecossistemas amazônicos (Terra Firme, Terra Preta e Várzea) no segundo semestre de 2008. |
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Palavras-Chave: |
Cultivo; Eco-fisiologia. |
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Thesagro: |
Artemisia Annua; Planta Medicinal. |
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Thesaurus NAL: |
malaria. |
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Categoria do assunto: |
-- |
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URL: |
https://www.abhorticultura.com.br/eventosx/trabalhos/ev_3/A2087_T3274_Comp.pdf
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Marc: |
LEADER 00897naa a2200217 a 4500
001 1664761
005 2009-08-11
008 2009 bl --- 0-- u #d
100 1 $aCHAVES, F. C. M.
245 $aProdução de biomassa de Artemisia annua L. em três ecossistemas amazônicos.
260 $c2009
520 $aAvaliou-se a produção de Artemisia annua em três ecossistemas amazônicos (Terra Firme, Terra Preta e Várzea) no segundo semestre de 2008.
650 $amalaria
650 $aArtemisia Annua
650 $aPlanta Medicinal
653 $aCultivo
653 $aEco-fisiologia
700 1 $aMAGALHÃES, P. M. de
700 1 $aHIDALGO, A. de F.
700 1 $aPOHLIT, A. M.
773 $tIn: CONGRESSO BRASILEIRO DE OLERICULTURA, 49., 2009, Águas de Lindóia. Água na horticultura: novas atitudes e uso sustentável. Horticultura Brasileira$gv. 27, n. 2, S2564-S2569, ago. 2009. Suplemento.
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Embrapa Amazônia Ocidental (CPAA) |
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