Data/hora: 04/06/2024 07:38:06

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 08/10/2009
Data da última atualização: | 04/07/2016
Autoria: | LIMA-VERDE, I. B.; MATOS, M. H. T.; BRUNO, J. B.; MARTINS, F. S.; SANTOS, R. R.; BÁO, S. N.; LUQUE, M. C. A.; VIEIRA, G. A. B.; SILVEIRA, E. R.; RODRIGUES, A. P. R.; FIGUEIREDO, J. R.; OLIVEIRA, M. A. L.; LIMA, P. F.
Título: | Effects of alpha-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured.
Ano de publicação: | 2009
Fonte/Imprenta: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 61, n. 1, p. 57-65, abr. 2009.
Idioma: | Inglês
Conteúdo: | The effects of á-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without á-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15ìM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that á-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture.
Palavras-Chave: | Folículo.
Thesagro: | Antioxidante; Caprino; Hormônio; Reprodução Animal.
Categoria do assunto: | --
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (22215-1) - ADD - AP - -- -  -  -  - DIS