Data/hora: 20/06/2024 20:45:49

Biblioteca(s): 	Embrapa Agroenergia.	
Data corrente: 	10/12/2021	
Data da última atualização: 	10/12/2021	
Autoria: 	RAMOS, T. G. S.; JUSTEN, F.; CARNEIRO, C. V. G. C.; HONORATO, V. M.; FRANCO, P. F.; VIEIRA, F. S.; TRICHEZ, D.; RODRIGUES, C. M.; ALMEIDA, J. R. M. de.	
Afiliação: 	TALITA G.S. RAMOS, Universidade de Brasília; FERNANDA JUSTEN, Universidade de Brasília; CLARA V. G. C. CARNEIRO, Universidade de Brasília; VICTOR M. HONORATO, Universidade de Brasília; PAULA FERNANDES FRANCO, CNPAE; FLÁVIA S. VIEIRA; DÉBORA TRICHEZ; CLENILSON MARTINS RODRIGUES, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE.	
Título: 	Xylonic acid production by recombinant Komagataella phaffii strains engineered with newly identified xylose dehydrogenases.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Bioresource Technology Reports, v. 16, 100825, Dec. 2021.	
Páginas: 	6 p.	
Descrição Física: 	PDF: il.	
ISSN: 	2589-014X	
DOI: 	https://doi.org/10.1016/j.biteb.2021.100825	
Idioma: 	Inglês	
Conteúdo: 	Abstract: Microbial production of xylonic acid can be achieved via xylose oxidation catalyzed by the xylose dehydrogenase enzyme. In the present study, 11 putative xylose dehydrogenases (XDH) from bacterial and fungi were identified through in silico analysis using the sequences of the two XDH previously described. Among them, 6 genes were successfully cloned and expressed in Komagataella phaffii (Pichia pastoris) as demonstrated by the ability of the strains to produce xylonic acid. The best strain was capable of producing up to 37.1 ± 1.9 and 11.7 ± 1.6 g/L of xylonic acid with yields of 0.96 ± 0.02 and 0.40 ± 0.06 g/g in mineral medium and sugarcane bagasse hydrolysate, respectively. The results presented here demonstrated the functionality of 6 newly identified xylose dehydrogenases, increasing the number of XDH described. In addition, the efficiency of K. phaffii as a xylonic acid producer is showed for the first time.	
Palavras-Chave: 	Biomass hydrolysate; Komagataella phaffii; Xylonic acid; Xylose dehydrogenase.	
Thesaurus Nal: 	Biomass; Hydrolysates.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Agroenergia (CNPAE)	
Exemplares: 	CNPAE - (3965-1) - UPC - AP - DD -  -  -  - DIS