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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
18/05/2023 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SHIOSE, L.; VIDAL, M. S.; HERINGER, A. S; VESPOLI, L. S.; SILVEIRA, V.; BALDANI, J. I.; JESUS, E. da C. |
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Afiliação: |
LUMI SHIOSE, UFRJ; MARCIA SOARES VIDAL, CNPAB; ANGELO S. HERINGER, UENF; LUCIANO S. VESPOLI, UENF; VANILDO SILVEIRA, UENF; JOSE IVO BALDANI, CNPAB; EDERSON DA CONCEICAO JESUS, CNPAB. |
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Título: |
Proteomic analysis of common bean (Phaseolus vulgaris L.) leaves showed a more stable metabolism in a variety responsive to biological nitrogen fixation. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Symbiosis, Published: 10 May 2023. |
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ISSN: |
0334-5114 |
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DOI: |
https://doi.org/10.1007/s13199-023-00921-z |
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Idioma: |
Inglês |
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Conteúdo: |
The common bean (Phaseolus vulgaris L.) can supply part of its N demand through biological nitrogen fixation (BNF) in symbiosis with rhizobia. However, the responses to inoculation and BNF's contribution to plant nutrition vary with the plant genotype. Here, we examined the proteome of two common bean genotypes (Ouro Negro and BRS Radiante) to determine shifts in leaf metabolism with two N sources: mineral N and inoculation with Rhizobium tropici CIAT899 (= SEMIA 4077 = BR 322). Data are available via ProteomeXchange with the identifier PXD034817. Through UPLC-MS/MS analysis, we iden- tified 917 proteins, 338 of which are differentially expressed. Here we focus on the 64 and 22 proteins differentially expressed in BRS Radiante and Ouro Negro, respectively, when comparing the proteome profiles of inoculated and N-fertilized plants. Different pathways in P. vulgaris were affected positively and negatively in BRS Radiante during symbiosis: glutathione metabolism, cysteine and methionine metabolism, phenylpropanoid biosynthesis, sulfur metabolism, peroxisome pentose and glucuronate interconversions, amino sugar and nucleotide sugar metabolism, carbon metabolism, and phagosome were the identified pathways. However, only three pathways were affected in Ouro Negro: peroxisome, endocytosis, and sulfur metabolism. Ouro Negro shows a less affected metabolism, which may lead to a more successful symbiosis. Proteins related to defense response were also induced in BRS Radiante, implying that their activation in this genotype may be partially responsible for its less efficient symbiosis. It is important to consider the difference in the life cycles of the two cultivars, as Ouro Negro had more time to adapt to the symbiosis than BRS Radiante. These results provide a basis for future studies to identify and explore biochemical mechanisms of nitrogen fixation in common beans beyond the roots. MenosThe common bean (Phaseolus vulgaris L.) can supply part of its N demand through biological nitrogen fixation (BNF) in symbiosis with rhizobia. However, the responses to inoculation and BNF's contribution to plant nutrition vary with the plant genotype. Here, we examined the proteome of two common bean genotypes (Ouro Negro and BRS Radiante) to determine shifts in leaf metabolism with two N sources: mineral N and inoculation with Rhizobium tropici CIAT899 (= SEMIA 4077 = BR 322). Data are available via ProteomeXchange with the identifier PXD034817. Through UPLC-MS/MS analysis, we iden- tified 917 proteins, 338 of which are differentially expressed. Here we focus on the 64 and 22 proteins differentially expressed in BRS Radiante and Ouro Negro, respectively, when comparing the proteome profiles of inoculated and N-fertilized plants. Different pathways in P. vulgaris were affected positively and negatively in BRS Radiante during symbiosis: glutathione metabolism, cysteine and methionine metabolism, phenylpropanoid biosynthesis, sulfur metabolism, peroxisome pentose and glucuronate interconversions, amino sugar and nucleotide sugar metabolism, carbon metabolism, and phagosome were the identified pathways. However, only three pathways were affected in Ouro Negro: peroxisome, endocytosis, and sulfur metabolism. Ouro Negro shows a less affected metabolism, which may lead to a more successful symbiosis. Proteins related to defense response were also induced in BRS Radiante, implying... Mostrar Tudo |
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Palavras-Chave: |
Protein expression; Symbiotic Nitrogen Fixation. |
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Thesagro: |
Phaseolus Vulgaris. |
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Thesaurus Nal: |
Mass spectrometry. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02761naa a2200265 a 4500
001 2153835
005 2023-05-18
008 2023 bl uuuu u00u1 u #d
022 $a0334-5114
024 7 $ahttps://doi.org/10.1007/s13199-023-00921-z$2DOI
100 1 $aSHIOSE, L.
245 $aProteomic analysis of common bean (Phaseolus vulgaris L.) leaves showed a more stable metabolism in a variety responsive to biological nitrogen fixation.$h[electronic resource]
260 $c2023
520 $aThe common bean (Phaseolus vulgaris L.) can supply part of its N demand through biological nitrogen fixation (BNF) in symbiosis with rhizobia. However, the responses to inoculation and BNF's contribution to plant nutrition vary with the plant genotype. Here, we examined the proteome of two common bean genotypes (Ouro Negro and BRS Radiante) to determine shifts in leaf metabolism with two N sources: mineral N and inoculation with Rhizobium tropici CIAT899 (= SEMIA 4077 = BR 322). Data are available via ProteomeXchange with the identifier PXD034817. Through UPLC-MS/MS analysis, we iden- tified 917 proteins, 338 of which are differentially expressed. Here we focus on the 64 and 22 proteins differentially expressed in BRS Radiante and Ouro Negro, respectively, when comparing the proteome profiles of inoculated and N-fertilized plants. Different pathways in P. vulgaris were affected positively and negatively in BRS Radiante during symbiosis: glutathione metabolism, cysteine and methionine metabolism, phenylpropanoid biosynthesis, sulfur metabolism, peroxisome pentose and glucuronate interconversions, amino sugar and nucleotide sugar metabolism, carbon metabolism, and phagosome were the identified pathways. However, only three pathways were affected in Ouro Negro: peroxisome, endocytosis, and sulfur metabolism. Ouro Negro shows a less affected metabolism, which may lead to a more successful symbiosis. Proteins related to defense response were also induced in BRS Radiante, implying that their activation in this genotype may be partially responsible for its less efficient symbiosis. It is important to consider the difference in the life cycles of the two cultivars, as Ouro Negro had more time to adapt to the symbiosis than BRS Radiante. These results provide a basis for future studies to identify and explore biochemical mechanisms of nitrogen fixation in common beans beyond the roots.
650 $aMass spectrometry
650 $aPhaseolus Vulgaris
653 $aProtein expression
653 $aSymbiotic Nitrogen Fixation
700 1 $aVIDAL, M. S.
700 1 $aHERINGER, A. S
700 1 $aVESPOLI, L. S.
700 1 $aSILVEIRA, V.
700 1 $aBALDANI, J. I.
700 1 $aJESUS, E. da C.
773 $tSymbiosis, Published: 10 May 2023.
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Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 292 | |
| 121. |  | MENEZES, C.; GONÇALVES, T.; ALQUÉRES, S.; ROUWS, L. F. M.; SERRATO, R.; VIDAL, M. S.; BALDANI, J. I. Gluconacetobacter diazotrophicus exopolysaccharide protects bacterial cells against oxidative stress in vitro and during rice plant colonization Plant and Soil, v. 416, n. 1–2, p. 133–147, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 124. | | MENESES, C. H. S. G.; ROUWS, L. F. M.; ARAUJO, J. L. S. de; VIDAL, M. S.; BALDANI, J. I. GumD gene involved in the initial steps of rice root colonization by Gluconacetobaceter diazotrophicus PAL5. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SlV.19| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 125. | | MENESES, C. H. S. G.; ROUWS, L. F. M.; SIMÕES-ARAÚJO, J. L.; VIDAL, M. S.; BALDANI, J. I. O gene gumD de Gluconacetobacter diazotrophicus (PAL5T) é essencial para colonização em plântulas de arroz. In: SEMANA CIENTÍFICA JOHANNA DOBEREINER, 9., 19 a 23 de outubro de 2009, Seropédica. Ciência no Brasil: desafios, avanços e aplicações. Seropédica: Embrapa Agrobiologia, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 127. | | FERREIRA, V. de L.; GALISA, P. de S.; MACEDO, A. V. de M.; VIDAL, M. S.; SIMÕES-ARAÚJO, J. L. Isolamento de genes diferencialmente expressos de Gluconacetobacter diazotrophicus cultivadas com diferentes fontes de carbono. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO, 18., 2008, Seropédica, RJ. Seropédica, RJ, 2008 Parceria: UFRRJ| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 133. | | SILVA, J. R. DA; GUEDES, H. V.; BALDANI, J. I.; VIDAL, M. S.; BALDANI, V. L. D. Envolvimento da proteína EnvZ nos processos de osmotolerância e fixação biológica de nitrogênio em Gluconacetobacter diazotrophicus. SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 11., 2011, Seropédica. Mudanças climáticas, desastres naturais e prevenção de riscos: resumos... Seropédica: Embrapa Agrobiologia, 2011. 43 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 134. | | SOARES, C. DE P.; FERREIRA, J. DE. P.; PEIXOTO, R. C. L.; VIDAL, M. S.; BALDANI, J. I. Envolvimento do sistema TonB-ExbB-ExbD1-ExbD2 no transporte de ferro em Gluconacetobacter diazotrophicus SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 11., 2011, Seropédica. Mudanças climáticas, desastres naturais e prevenção de riscos: resumos... Seropédica: Embrapa Agrobiologia, 2011. 44 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 140. | | GALVÃO, P.; MELO, L. H. de; SILVA-SILVA, A.; BISCH, P.; BALDANI, J. I.; VIDAL, M. S. Differential proteomic analysis of an over producing carbohydrate bacteria mutant derived from the diazotrophic Gluconacetobacter diazotrophicus strain PAL5 In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 19., 2015, Pacific Grove, CA, USA. Abstract... Califórnia: USDA, 2015| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 292 | |
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