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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
09/11/2021 |
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Data da última atualização: |
09/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOARES, I. C.; PACHECO, R. S.; SILVA, C. G. N. da; SANTOS, R. S.; BALDANI, J. I.; URQUIAGA, S.; VIDAL, M. S.; ARAUJO, J. L. S. de. |
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Afiliação: |
ISIS CAPELLA SOARES, UFRRJ; RAFAEL SANCHES PACHECO, UFRRJ; CLEUDISON GABRIEL NASCIMENTO DA SILVA, UFLA; RAFAEL SALAZAR SANTOS, UFRRJ; JOSE IVO BALDANI, CNPAB; SEGUNDO SACRAMENTO U CABALLERO, CNPAB; MARCIA SOARES VIDAL, CNPAB; JEAN LUIZ SIMOES DE ARAUJO, CNPAB. |
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Título: |
Real-time PCR method to quantify Sp245 strain of Azospirillum baldaniorum on Brachiaria grasses under field conditions. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Plant and Soil, 06 September 2021 |
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ISSN: |
0032-079X |
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DOI: |
https://doi.org/10.1007/s11104-021-05137-y |
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Idioma: |
Inglês |
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Conteúdo: |
Bacterial quantification by qPCR is considered the gold standard for microbial molecular diagnosis. However, a fundamental pre-requisite in this methodology is the designing of specific primers for the bacterium of interest. With the increase in bacterial genome sequencing data in the recent years, it has become possible to design specific primers that can be used to quantify different strains of the same bacterial species. Methods: To develop a real-time PCR (qPCR) protocol for the specific quantification of Azospirillum baldaniorum Sp245 strain (old Azospirillum brasilense), the Sp245 genome sequence was fragmented into small contigs with 500 base pairs each, and analyzed for similarity against the NCBI non-redundant database. A. baldaniorum-specific contigs were used to design the primers. The best pair of primers was used to quantify these bacteria after inoculation in different cultivars of Brachiaria, grown under field conditions. Results: Our results showed that the primer pair Sp245p10 was highly specific for the Sp245 strain in the Brachiaria root and shoot field under different conditions. The qPCR assay using these primers showed differences among cultivars in the number of bacteria detected in plants after inoculation. Additionally, the number of bacteria observed in the roots was higher than that in the shoots. Conclusion: The qPCR methodology using a Sp245 strain-specific primer may be used to monitor A. baldaniorum inoculated into other plants and may find potential application in field experiments. MenosBacterial quantification by qPCR is considered the gold standard for microbial molecular diagnosis. However, a fundamental pre-requisite in this methodology is the designing of specific primers for the bacterium of interest. With the increase in bacterial genome sequencing data in the recent years, it has become possible to design specific primers that can be used to quantify different strains of the same bacterial species. Methods: To develop a real-time PCR (qPCR) protocol for the specific quantification of Azospirillum baldaniorum Sp245 strain (old Azospirillum brasilense), the Sp245 genome sequence was fragmented into small contigs with 500 base pairs each, and analyzed for similarity against the NCBI non-redundant database. A. baldaniorum-specific contigs were used to design the primers. The best pair of primers was used to quantify these bacteria after inoculation in different cultivars of Brachiaria, grown under field conditions. Results: Our results showed that the primer pair Sp245p10 was highly specific for the Sp245 strain in the Brachiaria root and shoot field under different conditions. The qPCR assay using these primers showed differences among cultivars in the number of bacteria detected in plants after inoculation. Additionally, the number of bacteria observed in the roots was higher than that in the shoots. Conclusion: The qPCR methodology using a Sp245 strain-specific primer may be used to monitor A. baldaniorum inoculated into other plants and may find pot... Mostrar Tudo |
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Palavras-Chave: |
Azospirillum baldanioru; Plant growth promoting bacteria; Quantification; Signal grass. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02397naa a2200277 a 4500
001 2135952
005 2021-11-09
008 2021 bl uuuu u00u1 u #d
022 $a0032-079X
024 7 $ahttps://doi.org/10.1007/s11104-021-05137-y$2DOI
100 1 $aSOARES, I. C.
245 $aReal-time PCR method to quantify Sp245 strain of Azospirillum baldaniorum on Brachiaria grasses under field conditions.$h[electronic resource]
260 $c2021
520 $aBacterial quantification by qPCR is considered the gold standard for microbial molecular diagnosis. However, a fundamental pre-requisite in this methodology is the designing of specific primers for the bacterium of interest. With the increase in bacterial genome sequencing data in the recent years, it has become possible to design specific primers that can be used to quantify different strains of the same bacterial species. Methods: To develop a real-time PCR (qPCR) protocol for the specific quantification of Azospirillum baldaniorum Sp245 strain (old Azospirillum brasilense), the Sp245 genome sequence was fragmented into small contigs with 500 base pairs each, and analyzed for similarity against the NCBI non-redundant database. A. baldaniorum-specific contigs were used to design the primers. The best pair of primers was used to quantify these bacteria after inoculation in different cultivars of Brachiaria, grown under field conditions. Results: Our results showed that the primer pair Sp245p10 was highly specific for the Sp245 strain in the Brachiaria root and shoot field under different conditions. The qPCR assay using these primers showed differences among cultivars in the number of bacteria detected in plants after inoculation. Additionally, the number of bacteria observed in the roots was higher than that in the shoots. Conclusion: The qPCR methodology using a Sp245 strain-specific primer may be used to monitor A. baldaniorum inoculated into other plants and may find potential application in field experiments.
653 $aAzospirillum baldanioru
653 $aPlant growth promoting bacteria
653 $aQuantification
653 $aSignal grass
700 1 $aPACHECO, R. S.
700 1 $aSILVA, C. G. N. da
700 1 $aSANTOS, R. S.
700 1 $aBALDANI, J. I.
700 1 $aURQUIAGA, S.
700 1 $aVIDAL, M. S.
700 1 $aARAUJO, J. L. S. de
773 $tPlant and Soil, 06 September 2021
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
20/12/1996 |
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Data da última atualização: |
31/03/2015 |
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Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
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Autoria: |
ALBERTO, G.; ALFAYA JÚNIOR, H.; PORTELLA, J. da S.; OLIVEIRA, O. L. P. de. |
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Título: |
Valor nutritivo e consumo voluntário de silagens de capim elefante (Pennisetum purpureum Schum. cv. Cameroon). |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Bagé: EMBRAPA-CPPSUL, 1995. |
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Páginas: |
30 p. |
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Série: |
(EMBRAPA-CPPSUL. Boletim de pesquisa, 16). |
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ISSN: |
0103-3743 |
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Idioma: |
Português |
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Conteúdo: |
Silagem de Capim-Elefante submetidas a diferentes tratamentos: a) sem emurchamento; b) com emurchamento; c) sem emurchamento + 8% de grãos de sorgo moído; d) com emurchamento + 8% de grãos de sorgo moído foram avaliadas quanto ao seu valor nutritivo ao seu aproveitamento aparente, com ovinos (machos castrados), por meio de ensaio de consumo voluntário, digestibildiade "in vitro" e balanço de nitrogênio. |
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Palavras-Chave: |
Consumo voluntário; cv; pennisetum purpureum Schum. |
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Thesagro: |
Consumo; Digestibilidade; Nitrogênio; Nutrição Animal; Pennisetum Purpureum; Silagem; Valor Nutritivo. |
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Thesaurus NAL: |
Cameroon. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/109800/1/VALOR-NUTRITIVO-E-CONSUMO.pdf
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Marc: |
LEADER 01301nam a2200313 a 4500
001 1225739
005 2015-03-31
008 1995 bl uuuu u0uu1 u #d
022 $a0103-3743
100 1 $aALBERTO, G.
245 $aValor nutritivo e consumo voluntário de silagens de capim elefante (Pennisetum purpureum Schum. cv. Cameroon).
260 $aBagé: EMBRAPA-CPPSUL$c1995
300 $a30 p.
490 $a(EMBRAPA-CPPSUL. Boletim de pesquisa, 16).
520 $aSilagem de Capim-Elefante submetidas a diferentes tratamentos: a) sem emurchamento; b) com emurchamento; c) sem emurchamento + 8% de grãos de sorgo moído; d) com emurchamento + 8% de grãos de sorgo moído foram avaliadas quanto ao seu valor nutritivo ao seu aproveitamento aparente, com ovinos (machos castrados), por meio de ensaio de consumo voluntário, digestibildiade "in vitro" e balanço de nitrogênio.
650 $aCameroon
650 $aConsumo
650 $aDigestibilidade
650 $aNitrogênio
650 $aNutrição Animal
650 $aPennisetum Purpureum
650 $aSilagem
650 $aValor Nutritivo
653 $aConsumo voluntário
653 $acv
653 $apennisetum purpureum Schum
700 1 $aALFAYA JÚNIOR, H.
700 1 $aPORTELLA, J. da S.
700 1 $aOLIVEIRA, O. L. P. de
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