| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Milho e Sorgo. |
|
Data corrente: |
26/01/2016 |
|
Data da última atualização: |
02/02/2016 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SILVEIRA, R. D.; ABREU, F. R. M.; MAMIDI, S.; McCLEAN, P. E.; VIANELLO, R. P.; LANNA, A. C.; CARNEIRO, N. P.; BRONDANI, C. |
|
Afiliação: |
NEWTON PORTILHO CARNEIRO, CNPMS. |
|
Título: |
Expression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa). |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 14, n. 3, p. 8181-8200, 2015. |
|
DOI: |
10.4238/2015.July.27.6 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Gene expression related to drought response in the leaf tissues of two Brazilian upland cultivars, the drought-tolerant Douradão and the drought-sensitive Primavera, was analyzed. RNA-seq identified 27,618 transcripts in the Douradão cultivar, with 24,090 (87.2%) homologous to the rice database, and 27,221 transcripts in the Primavera cultivar, with 23,663 (86.9%) homologous to the rice database. Gene-expression analysis between control and water-deficient treatments revealed 493 and 1154 differentially expressed genes in Douradão and Primavera cultivars, respectively. Genes exclusively expressed under drought were identified for Douradão, including two genes of particular interest coding for the protein peroxidase precursor, which is involved in three distinct metabolic pathways. Comparisons between the two drought-exposed cultivars revealed 2314 genes were differentially expressed (978 upregulated, 1336 downregulated in Douradão). Six genes distributed across 4 different transcription factor families (bHLH, MYB, NAC, and WRKY) were identified, all of which were upregulated in Douradão compared to Primavera during drought. Most of the genes identified in Douradão activate metabolic pathways responsible for production of secondary metabolites and genes coding for enzymatically active signaling receptors. Quantitative PCR validation showed that most gene expression was in agreement with computational prediction of these transcripts. The transcripts identified here will define molecular markers for identification of Cis-acting elements to search for allelic variants of these genes through analysis of polymorphic SNPs in GenBank accessions of upland rice, aiming to develop cultivars with the best combination of these alleles, resulting in materials with high yield potential in the event of drought during the reproductive phase. MenosGene expression related to drought response in the leaf tissues of two Brazilian upland cultivars, the drought-tolerant Douradão and the drought-sensitive Primavera, was analyzed. RNA-seq identified 27,618 transcripts in the Douradão cultivar, with 24,090 (87.2%) homologous to the rice database, and 27,221 transcripts in the Primavera cultivar, with 23,663 (86.9%) homologous to the rice database. Gene-expression analysis between control and water-deficient treatments revealed 493 and 1154 differentially expressed genes in Douradão and Primavera cultivars, respectively. Genes exclusively expressed under drought were identified for Douradão, including two genes of particular interest coding for the protein peroxidase precursor, which is involved in three distinct metabolic pathways. Comparisons between the two drought-exposed cultivars revealed 2314 genes were differentially expressed (978 upregulated, 1336 downregulated in Douradão). Six genes distributed across 4 different transcription factor families (bHLH, MYB, NAC, and WRKY) were identified, all of which were upregulated in Douradão compared to Primavera during drought. Most of the genes identified in Douradão activate metabolic pathways responsible for production of secondary metabolites and genes coding for enzymatically active signaling receptors. Quantitative PCR validation showed that most gene expression was in agreement with computational prediction of these transcripts. The transcripts identified here will define... Mostrar Tudo |
|
Thesagro: |
Arroz; Gene; Oryza sativa; Resistência a seca; Variedade. |
|
Categoria do assunto: |
--
S Ciências Biológicas |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/137806/1/Expression-drought.pdf
|
|
Marc: |
LEADER 02671naa a2200277 a 4500
001 2035196
005 2016-02-02
008 2015 bl uuuu u00u1 u #d
024 7 $a10.4238/2015.July.27.6$2DOI
100 1 $aSILVEIRA, R. D.
245 $aExpression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa).$h[electronic resource]
260 $c2015
520 $aGene expression related to drought response in the leaf tissues of two Brazilian upland cultivars, the drought-tolerant Douradão and the drought-sensitive Primavera, was analyzed. RNA-seq identified 27,618 transcripts in the Douradão cultivar, with 24,090 (87.2%) homologous to the rice database, and 27,221 transcripts in the Primavera cultivar, with 23,663 (86.9%) homologous to the rice database. Gene-expression analysis between control and water-deficient treatments revealed 493 and 1154 differentially expressed genes in Douradão and Primavera cultivars, respectively. Genes exclusively expressed under drought were identified for Douradão, including two genes of particular interest coding for the protein peroxidase precursor, which is involved in three distinct metabolic pathways. Comparisons between the two drought-exposed cultivars revealed 2314 genes were differentially expressed (978 upregulated, 1336 downregulated in Douradão). Six genes distributed across 4 different transcription factor families (bHLH, MYB, NAC, and WRKY) were identified, all of which were upregulated in Douradão compared to Primavera during drought. Most of the genes identified in Douradão activate metabolic pathways responsible for production of secondary metabolites and genes coding for enzymatically active signaling receptors. Quantitative PCR validation showed that most gene expression was in agreement with computational prediction of these transcripts. The transcripts identified here will define molecular markers for identification of Cis-acting elements to search for allelic variants of these genes through analysis of polymorphic SNPs in GenBank accessions of upland rice, aiming to develop cultivars with the best combination of these alleles, resulting in materials with high yield potential in the event of drought during the reproductive phase.
650 $aArroz
650 $aGene
650 $aOryza sativa
650 $aResistência a seca
650 $aVariedade
700 1 $aABREU, F. R. M.
700 1 $aMAMIDI, S.
700 1 $aMcCLEAN, P. E.
700 1 $aVIANELLO, R. P.
700 1 $aLANNA, A. C.
700 1 $aCARNEIRO, N. P.
700 1 $aBRONDANI, C.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 14, n. 3, p. 8181-8200, 2015.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Agrobiologia. |
|
Data corrente: |
15/08/2005 |
|
Data da última atualização: |
15/08/2005 |
|
Autoria: |
SILVA, V. V.; GUERRA, J. G. M.; ALMEIDA, D. L. de; RIBEIRO, R. de L. D. |
|
Título: |
Efeito do pré-cultivo de adubos verdes na produção orgânica de brócolos em sistema plantio direto. |
|
Ano de publicação: |
2002 |
|
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE OLERICULTURA, 42., CONGRESSO LATINO AMERICANO DE HORTICULTURA, 11., 2002, Uberlândia. Resumos... Uberlândia: Sociedade de Olericultura do Brasil, 2002. Horticultura Brasileira, Brasília, v. 20, n. 2, p. 295. Resumo. Suplemento. |
|
Idioma: |
Português |
|
Thesagro: |
Adubação Verde; Brócolo; Plantio Direto. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00760naa a2200181 a 4500
001 1625046
005 2005-08-15
008 2002 bl --- 0-- u #d
100 1 $aSILVA, V. V.
245 $aEfeito do pré-cultivo de adubos verdes na produção orgânica de brócolos em sistema plantio direto.
260 $c2002
650 $aAdubação Verde
650 $aBrócolo
650 $aPlantio Direto
700 1 $aGUERRA, J. G. M.
700 1 $aALMEIDA, D. L. de
700 1 $aRIBEIRO, R. de L. D.
773 $tIn: CONGRESSO BRASILEIRO DE OLERICULTURA, 42., CONGRESSO LATINO AMERICANO DE HORTICULTURA, 11., 2002, Uberlândia. Resumos... Uberlândia: Sociedade de Olericultura do Brasil, 2002. Horticultura Brasileira, Brasília$gv. 20, n. 2, p. 295. Resumo. Suplemento.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
