Data/hora: 22/06/2024 19:06:37

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 01/09/2014
Data da última atualização: | 09/12/2014
Autoria: | MÜLLER, B. S. de F.; SAKAMOTO, T.; MENEZES, I. P. P. de; PRADO, G. S.; MARTINS, W. S.; BRONDANI, C.; BARROS, E. G. de; VIANELLO, R. P.
Afiliação: | BÁRBARA SALOMÃO DE FARIA MÜLLER, UFV; TETSU SAKAMOTO, UFMG; IVANDILSON PESSOA PINTO DE MENEZES, INSTITUTO FEDERAL GOIANO, Urutaí-GO; GUILHERME SOUZA PRADO, bolsista CNPAF; WELLINGTON SANTOS MARTINS, UFG; CLAUDIO BRONDANI, CNPAF; EVERALDO GONÇALVES DE BARROS, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ROSANA PEREIRA VIANELLO, CNPAF.
Título: | Analysis of BAC-end sequences in common bean (Phaseolus vulgaris L.) towards the development and characterization of long motifs SSRs.
Ano de publicação: | 2014
Fonte/Imprenta: | Plant Molecular Biology, Dordrecht, v. 86, n. 4/5, p. 455-470, Nov. 2014.
DOI: | 10.1007/s11103-014-0240-7
Idioma: | Inglês
Conteúdo: | The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number of bacterial artificial chromosome (BAC) were sequenced, annotated and the presence of repetitive sequences was determined. In total, 52,270 BESs (BAC-end sequences), equivalent to 32 Mbp (~6 %) of the genome, were processed. In total, 3,789 BES-SSRs were identified, with a distribution of one SSR (simple sequence repeat) per 8.36 kbp and 2,000 were suitable for the development of SSRs, of which 194 were evaluated in low-resolution screening. From 40 BES-SSRs based on long motifs SSRs (&#8805;trinucleotides) analyzed in high-resolution genotyping, 34 showed an equally good amplification for the Andean and for the Mesoamerican genepools, exhibiting an average gene diversity (H E) of 0.490 and 5.59 alleles/locus, of which six classified as Class I showed a H E &#8805; 0.7. The PCoA and structure analysis allowed to discriminate the gene pools (K = 2, FST = 0.733). From the 52,270 BESs, 2 % corresponded to transcription factors and 3 % to transposable elements. Putative functions for 24,321 BESs were identified and for 19,363 were assigned functional categories (gene ontology). This study identified highly polymorphic BES-SSRs containing tri- to hexanucleotides motifs and bringing together relevant genetic characteristics useful for breeding programs. Additionally, the BESs were incorporated into the international genome-sequencing project for the common bean.
Thesagro: | Feijão; Genética molecular; Phaseolus vulgaris.
Thesaurus Nal: | Beans; Fabaceae; Genomics; Microsatellite repeats; Molecular genetics; Transcription factors; Transposons.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (32957-1) - UPC - AP - DD - 2014.052 -  - 2014.052 - DIS