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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
18/02/2014 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ARASHIRO, E. K. N.; PALHÃO, M. P.; WOHLRES-VIANA, S.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; HENRY, M. R. J. M.; VIANA, J. H. M. |
Afiliação: |
EDUARDO KENJI N. ARASHIRO, UFMG; MILLER PEREIRA PALHÃO, UNIFENAS; SABINE WOHLRES-VIANA, UFJF; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARC ROGER JEAN MARIE HENRY, UFMG; JOAO HENRIQUE MOREIRA VIANA, CNPGL. |
Título: |
In vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Reproductive Biology and Endocrinology, v. 11, article 73, 2013. |
DOI: |
https://doi.org/10.1186/1477-7827-11-73 |
Idioma: |
Inglês |
Conteúdo: |
Background: Most studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters. Methods: In the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle. Results: In Trial I, the plasma recovery efficiency was similar (P > 0.05) for the volumes expected for 12 and 10 mm follicles, but decreased (P < 0.05) for smaller follicles (45.7+/-4.0%, 12.4+/-4.3% and 0.0+/-0.0% for 8, 6, and 4 mm follicles, respectively). Using the adaptation, the losses intrinsic to the aspiration system were similar for all follicle diameters. In Trial II, the expected and recovered volumes of FF were correlated (r = 0.89) and the efficiency of recovery was similar among follicles <12 mm, while larger follicles had a progressive increase in FF losses that was not related to the tubing system. In Trial III, the number of GC and amount of RNA obtained were not affected (P > 0.05) by follicle size, but differed according to breed (615,054+/-58,122 vs 458,095+/-36,407 for Holstein and Gir, respectively; P < 0.05). Conclusions: The adapted-OPU system can be successfully used for the in vivo collection of FF and GC from follicles of different diameters. This will enable further endocrine, cellular, and gene expression analyses. MenosBackground: Most studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters. Methods: In the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle. Results: In Trial I, the plasma recovery efficiency was similar (P > 0.05) for the v... Mostrar Tudo |
Palavras-Chave: |
Bovine; Follicle aspiration; Follicle biopsy. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03310naa a2200241 a 4500 001 1980418 005 2024-02-05 008 2013 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1186/1477-7827-11-73$2DOI 100 1 $aARASHIRO, E. K. N. 245 $aIn vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system.$h[electronic resource] 260 $c2013 520 $aBackground: Most studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters. Methods: In the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle. Results: In Trial I, the plasma recovery efficiency was similar (P > 0.05) for the volumes expected for 12 and 10 mm follicles, but decreased (P < 0.05) for smaller follicles (45.7+/-4.0%, 12.4+/-4.3% and 0.0+/-0.0% for 8, 6, and 4 mm follicles, respectively). Using the adaptation, the losses intrinsic to the aspiration system were similar for all follicle diameters. In Trial II, the expected and recovered volumes of FF were correlated (r = 0.89) and the efficiency of recovery was similar among follicles <12 mm, while larger follicles had a progressive increase in FF losses that was not related to the tubing system. In Trial III, the number of GC and amount of RNA obtained were not affected (P > 0.05) by follicle size, but differed according to breed (615,054+/-58,122 vs 458,095+/-36,407 for Holstein and Gir, respectively; P < 0.05). Conclusions: The adapted-OPU system can be successfully used for the in vivo collection of FF and GC from follicles of different diameters. This will enable further endocrine, cellular, and gene expression analyses. 653 $aBovine 653 $aFollicle aspiration 653 $aFollicle biopsy 700 1 $aPALHÃO, M. P. 700 1 $aWOHLRES-VIANA, S. 700 1 $aSIQUEIRA, L. G. B. 700 1 $aCAMARGO, L. S. de A. 700 1 $aHENRY, M. R. J. M. 700 1 $aVIANA, J. H. M. 773 $tReproductive Biology and Endocrinology$gv. 11, article 73, 2013.
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Registros recuperados : 68 | |
61. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BOITÉ, M. C.; NOGUEIRA, L. A. G.; PEREIRA, M. M.; BATISTA, R. I. T. P.; VIANA, S. W.; POLISSENI, J.; MOTA, G. B.; SERAPIÃO, R. V.; CARVALHO, B. C.; IGUMA, L. T.; SÁ, W. F.; VIANA, J. H. M.; CAMARGO, L. S. A. Avaliação da capacidade de desidratação e reidratação de embriões bovinos cultivados em diferentes meios. In: SEMANA DE BIOLOGIA, 30., 2007, Juiz de Fora. O mundo se tornou pequeno para você mas você não é grande para o mundo: anais. Juiz de Fora: DACBIO/UFJF, 2007. p. 117-123.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Gado de Leite. |
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62. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SOUSA, V. F. de; BELMINO, C. S.; SOUSA, C. A. F. de; CARNEIRO, J. D. S.; SILVA, P. H. S. da; VASCONCELOS, L. F. L.; SOUSA, H. U. de; MAIA, C. B.; ELOI, W. M.; VIANA, S. R. S.; BARBOSA, F. R.; MOREIRA, A. N.; TAVARES, S. C. C. de H.; MOUCO, M. A. do C.; SILVA, D. J.; ASSIS, J. S. de. Produção integrada de manga no Estado do Piauí: documentos de referência. Teresina: Embrapa Meio-Norte, 2005. 140 p. (Embrapa Meio-Norte. Documentos, 106).Biblioteca(s): Embrapa Semiárido. |
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63. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SOUSA, V. F. de; BELMINO, C. S.; SOUSA, C. A. F. de; CARNEIRO, J. da S.; SILVA, P. H. S. da; VASCONCELOS, L. F. L.; SOUSA, H. U. de; MAIA, C. B.; ELOI, W. M.; VIANA, S. R. S.; BARBOSA, F. R.; MOREIRA, A. N.; TAVARES, S. C. C. de H.; MOUCO, M. A. do C.; SILVA, D. J. da; ASSIS, J. S. de. Produção integrada de manga no Estado do Piauí: documentos de referência. Teresina: Embrapa Meio-Norte, 2005. 140 p. (Embrapa Meio-Norte. Documentos, 106).Biblioteca(s): Embrapa Meio-Norte. |
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64. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SOUSA, V. F. de; SOUSA, C. A. F. de; BELMINO, C. S.; CARNEIRO, J. da S.; SILVA, P. H. S. da; VASCONCELOS, L. F. L.; SOUSA, H. U. de; MAIA, C. B.; ELOI, W. M.; VIANA, S. R. S.; BARBOSA, F. R.; MOREIRA, A. N.; TAVARES, S. C. C. de H.; MOUCO, M. A. do C.; SILVA, D. J. da; ASSIS, J. S. de. Produção integrada de manga no Estado do Piauí. Teresina: Embrapa Meio-Norte, 2004. 44 p. (Embrapa Meio-Norte. Documentos, 90).Biblioteca(s): Embrapa Meio-Norte. |
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65. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SOUSA, V. F. de; SOUSA, C. A. F. de; BELMINO, C. S.; CARNEIRO, J. da S.; SILVA, P. H. S. da; VASCONCELOS, L. F. L.; SOUSA, H. U.; MAIA, C. B.; ELOI, W. M.; VIANA, S. R. S.; MOREIRA, F. R. B.; MOREIRA, A. N.; TAVARES, S. C. C. de H.; MOUCO, M. A. do C.; SILVA, D. J.; ASSIS, J. S. de. Produção integrada de manga no Estado do Piauí. Teresina: Embrapa Meio-Norte, 2004. 44 p. (Embrapa Meio-Norte. Documentos, 90).Biblioteca(s): Embrapa Semiárido. |
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66. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | IGUMA, L. T.; PEREIRA, M. M.; VIANA, S. W.; SALES, J. N. S.; QUINTAO, C. C. R.; PINTO, I. S. B.; SERAPIÃO, R. V.; BRITO, S. G.; CARVALHO, B. C. de; VIANA, J. H. M.; CAMARGO, L. S. de A.; MARTINS, M. F. Quantificação relativa de transcritos maternos em oocitos bovinos com e sem corpúsculo polar após maturação in vitro. In: Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, 25., 2011, Cumbuco. Resumos... p. 459Tipo: Resumo em Anais de Congresso |
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67. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | IGUMA, L. T.; PEREIRA, M. M.; WOHLRES-VIANA, S.; SALES, J. N. S.; QUINTAO, C. C. R.; PINTO, I. S. B.; SERAPIÃO, R. V.; BRITO, S. G.; CARAVALHO, B. C.; VIANA, J. H. M.; CAMARGO, L. S. de A.; MARTINS, M. F. Relative abundance of material transcripts in oocytes with and without polar body after in vitro maturation. In: Annual Meeting SBTE, 25., 2011. Abstracts...Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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68. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BOITÉ, M. C.; NOGUEIRA, L. A. G.; PEREIRA, M. M.; BATISTA, R. I. T. P.; VIANA, S. W.; POLISSENI, J.; MOTA, G. B.; SERAPIÃO, R. V.; CARVALHO, B. C.; IGUMA, L. T.; SÁ, W. F.; VIANA, J. H. M.; CAMARGO, L. S. A. Taxa de sobrevivência in vitro de embriões bovinos produzidos in vitro submetidos à vitrificação ou congelamento lento cm etilenoglicol. In: SEMANA DE BIOLOGIA, 30., 2007, Juiz de Fora. O mundo se tornou pequeno para você mas você não é grande para o mundo: anais. Juiz de Fora: DACBIO/UFJF, 2007. p. 238-241.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Gado de Leite. |
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Registros recuperados : 68 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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