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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
03/07/2021 |
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Data da última atualização: |
31/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIANA, J. H. M.; PEREIRA, N. E. S.; FARIA, O. A. C.; DIAS, L. R. O.; OLIVEIRA, E. R.; FERNANDES, C. A. C.; SIQUEIRA, L. G. B. |
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Afiliação: |
JOAO HENRIQUE MOREIRA VIANA, Cenargen; NATHALIA ELLEN SOUSA PEREIRA, UNB; OTAVIO AUGUSTO COSTA FARIA, UNB; LUZIA RENATA OLIVEIRA DIAS, UNB; EDUARDO RAMOS OLIVEIRA, UNIFENAS; CARLOS ANTONIO CARVALHO FERNANDES, UNIFENAS; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL. |
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Título: |
Active immunization against GnRH as an alternative therapeutic approach for the management of Bos indicus oocyte donors diagnosed with chronic cystic ovarian disease. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Theriogenology, v. 172, p. 133-141, 2021. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2021.06.014 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the effect of active immunization against GnRH in Nelore (Bos indicus) cows repeatedly used as oocyte donors that developed chronic cystic ovarian disease (COD). In the first experiment, ovarian and uterine features were first compared between COD cows (n ¼ 15) and healthy cows (n ¼ 22, cycling control group) from the same breed and herd. Cows with COD had a greater number of large (P < 0.0001) and medium follicles (P < 0.01) but lesser small follicles (P < 0.05) than cycling controls. Mucometra was diagnosed in 73.3% of COD cows, but in none of the controls. No difference in average thickness of the endometrium was detected between groups; however, endometrial thickness and mucometra score were negatively correlated (R ¼ 0.73, P ¼ 0.0029) in COD cows. In the second experiment, COD cows were randomly allocated into two experimental groups, which received two 1.0 mL SC injections of either an anti-GnRH vaccine (COD immunized group, n ¼ 8) or saline (COD control group, n ¼ 7), given 28 days apart. Cows were examined weekly by transrectal ultrasonography during nine consecutive weeks after the first injection to evaluate the number and distribution of follicles among size classes, endometrial thickness, and presence of clinical mucometra. Vaccination against GnRH resulted in a progressive suppression of follicle growth and a reduction in the average size of the largest follicle, as well as in the number of large follicles (P < 0.01) in COD immunized cows compared with COD controls from week 7 onwards. Conversely, the number of small follicles in the COD immunized group increased after week 5 and was greater (P ¼ 0.0023) than controls on week 9. Endometrial thickness and mucometra score were not affected (P > 0.05) by immunization against GnRH. In the third experiment, the COD immunized cows with effective suppression of follicle growth four weeks after the 2nd injection (n ¼ 6) were submitted to three consecutive ovum pick-up (OPU) sessions (weeks 10, 11, and 12) for in vitro embryo production (IVEP). Cumulus-oocyte complexes (COC) collected from slaughterhouse ovaries were used as controls for IVEP. COD cows with produced 25.0 ± 3.8 COC per OPU session with no apparent detrimental effect of anti-GnRH vaccine on oocyte developmental potential in vitro, i.e., we observed similar cleavage rate (P ¼ 0.5914) and greater blastocyst rate (P ¼ 0.0177) in immunized cows compared with COC from slaughterhouse controls. Finally, in the fourth experiment wave emergence and follicular dynamics after follicle ablation were compared between COD immunized cows with effective suppression of follicle growth and a subset (n ¼ 6) of the cycling, control group. No follicles grew beyond 4 mm diameter after follicle ablation in the COD immunized group, whereas a normal follicular wave emergence occurred in cycling controls. Antral follicle count was similar between cycling controls and COD immunized groups at 24 h and 96 h post-follicle ablation (P > 0.05), but greater in cycling controls at 48 h and 72 h post-follicle ablation (P < 0.05). In summary, our results suggest that active immunization against GnRH is effective to induce the regression of follicular cysts as well as increase the number of small follicles growing on the ovaries, in oocyte donors diagnosed with chronic COD, with no apparent negative effect on oocyte developmental potential in vitro. MenosThe aim of this study was to evaluate the effect of active immunization against GnRH in Nelore (Bos indicus) cows repeatedly used as oocyte donors that developed chronic cystic ovarian disease (COD). In the first experiment, ovarian and uterine features were first compared between COD cows (n ¼ 15) and healthy cows (n ¼ 22, cycling control group) from the same breed and herd. Cows with COD had a greater number of large (P < 0.0001) and medium follicles (P < 0.01) but lesser small follicles (P < 0.05) than cycling controls. Mucometra was diagnosed in 73.3% of COD cows, but in none of the controls. No difference in average thickness of the endometrium was detected between groups; however, endometrial thickness and mucometra score were negatively correlated (R ¼ 0.73, P ¼ 0.0029) in COD cows. In the second experiment, COD cows were randomly allocated into two experimental groups, which received two 1.0 mL SC injections of either an anti-GnRH vaccine (COD immunized group, n ¼ 8) or saline (COD control group, n ¼ 7), given 28 days apart. Cows were examined weekly by transrectal ultrasonography during nine consecutive weeks after the first injection to evaluate the number and distribution of follicles among size classes, endometrial thickness, and presence of clinical mucometra. Vaccination against GnRH resulted in a progressive suppression of follicle growth and a reduction in the average size of the largest follicle, as well as in the number of large follicles (P < 0.01) in COD ... Mostrar Tudo |
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Palavras-Chave: |
Follicle growth suppression; GnRH vaccine; In vitro embryo production; Nelore breed. |
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Thesagro: |
Bovino; Doença Animal; Gado Nelore. |
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Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 04414naa a2200289 a 4500
001 2133972
005 2021-08-31
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2021.06.014$2DOI
100 1 $aVIANA, J. H. M.
245 $aActive immunization against GnRH as an alternative therapeutic approach for the management of Bos indicus oocyte donors diagnosed with chronic cystic ovarian disease.$h[electronic resource]
260 $c2021
520 $aThe aim of this study was to evaluate the effect of active immunization against GnRH in Nelore (Bos indicus) cows repeatedly used as oocyte donors that developed chronic cystic ovarian disease (COD). In the first experiment, ovarian and uterine features were first compared between COD cows (n ¼ 15) and healthy cows (n ¼ 22, cycling control group) from the same breed and herd. Cows with COD had a greater number of large (P < 0.0001) and medium follicles (P < 0.01) but lesser small follicles (P < 0.05) than cycling controls. Mucometra was diagnosed in 73.3% of COD cows, but in none of the controls. No difference in average thickness of the endometrium was detected between groups; however, endometrial thickness and mucometra score were negatively correlated (R ¼ 0.73, P ¼ 0.0029) in COD cows. In the second experiment, COD cows were randomly allocated into two experimental groups, which received two 1.0 mL SC injections of either an anti-GnRH vaccine (COD immunized group, n ¼ 8) or saline (COD control group, n ¼ 7), given 28 days apart. Cows were examined weekly by transrectal ultrasonography during nine consecutive weeks after the first injection to evaluate the number and distribution of follicles among size classes, endometrial thickness, and presence of clinical mucometra. Vaccination against GnRH resulted in a progressive suppression of follicle growth and a reduction in the average size of the largest follicle, as well as in the number of large follicles (P < 0.01) in COD immunized cows compared with COD controls from week 7 onwards. Conversely, the number of small follicles in the COD immunized group increased after week 5 and was greater (P ¼ 0.0023) than controls on week 9. Endometrial thickness and mucometra score were not affected (P > 0.05) by immunization against GnRH. In the third experiment, the COD immunized cows with effective suppression of follicle growth four weeks after the 2nd injection (n ¼ 6) were submitted to three consecutive ovum pick-up (OPU) sessions (weeks 10, 11, and 12) for in vitro embryo production (IVEP). Cumulus-oocyte complexes (COC) collected from slaughterhouse ovaries were used as controls for IVEP. COD cows with produced 25.0 ± 3.8 COC per OPU session with no apparent detrimental effect of anti-GnRH vaccine on oocyte developmental potential in vitro, i.e., we observed similar cleavage rate (P ¼ 0.5914) and greater blastocyst rate (P ¼ 0.0177) in immunized cows compared with COC from slaughterhouse controls. Finally, in the fourth experiment wave emergence and follicular dynamics after follicle ablation were compared between COD immunized cows with effective suppression of follicle growth and a subset (n ¼ 6) of the cycling, control group. No follicles grew beyond 4 mm diameter after follicle ablation in the COD immunized group, whereas a normal follicular wave emergence occurred in cycling controls. Antral follicle count was similar between cycling controls and COD immunized groups at 24 h and 96 h post-follicle ablation (P > 0.05), but greater in cycling controls at 48 h and 72 h post-follicle ablation (P < 0.05). In summary, our results suggest that active immunization against GnRH is effective to induce the regression of follicular cysts as well as increase the number of small follicles growing on the ovaries, in oocyte donors diagnosed with chronic COD, with no apparent negative effect on oocyte developmental potential in vitro.
650 $aBovino
650 $aDoença Animal
650 $aGado Nelore
653 $aFollicle growth suppression
653 $aGnRH vaccine
653 $aIn vitro embryo production
653 $aNelore breed
700 1 $aPEREIRA, N. E. S.
700 1 $aFARIA, O. A. C.
700 1 $aDIAS, L. R. O.
700 1 $aOLIVEIRA, E. R.
700 1 $aFERNANDES, C. A. C.
700 1 $aSIQUEIRA, L. G. B.
773 $tTheriogenology$gv. 172, p. 133-141, 2021.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
06/08/2020 |
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Data da última atualização: |
04/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
RIBEIRO, P. C. de O.; MARÇAL, T. de S.; OLIVEIRA, I. C. M.; SCHAFFERT, R. E.; CARNEIRO, P. C. S.; OLIVEIRA, A. B. de; PARRELLA, R. A. da C. |
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Afiliação: |
Pedro César de Oliveira Ribeiro, Universidade Federal de Viçosa; Tiago de Souza Marçal, Universidade Federal de Viçosa; Isadora Cristina Martins Oliveira, Universidade Federal de Viçosa; ROBERT EUGENE SCHAFFERT, CNPMS; Pedro Crescêncio Souza Carneiro, Universidade Federal de Viçosa; Aluízio Borém de Oliveira, Universidade Federal de Viçosa; RAFAEL AUGUSTO DA COSTA PARRELLA, CNPMS. |
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Título: |
Insight into genetic potential of male sterile sweet sorghum A-lines for agroindustrial traits using tester R-lines. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Industrial Crops and Products, v. 153, article 112557, 2020. |
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DOI: |
https://doi.org/10.1016/j.indcrop.2020.112577 |
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Idioma: |
Inglês |
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Conteúdo: |
There is a growing demand for the development of alternative fuels from renewable sources, thus sweet sorghum an autogamous species has potential for bioethanol production. Traditionally recommended cultivars were varieties, however, with the development of cytoplasmic male sterile sweet sorghum lines, sweet sorghum hybrids have become economically viable. The sweet sorghum breeding program of the Brazilian Agricultural Research Corporation (Embrapa Milho e Sorgo) has been developing sweet sorghum hybrids for bioethanol production according to the scheme of A, B and R-lines are used, where A-lines are used as females in the production of hybrid seeds. A and B-lines are isogenic, which differ only its cytoplasm source, sterile and normal, respectively, and fertility restorers R-lines. Thus the objective of this work was to evaluate the genetic potential of new male-sterile sweet sorghum A-lines using testcross hybrids developed with two sweet sorghum R-line as testers for agroindustry characteristics for bioethanol production. Forty testcross hybrids were developed using 20 experimental A-lines and two testers sweet sorghum R-lines. Experiments were conducted at experimental stations in Sete Lagoas-MG and Nova Porteirinha-MG, to evaluate 20 A-lines, two 2 R-lines, the resulting 40 testcross hybrids, and two controls. The 64 treatments were evaluated for days to flowering, fresh biomass yield and total soluble solids (Brix). The data obtained were analyzed using the ASReml package, for the estimation of variance components and the prediction of genetic and non-genetic values via REML/BLUP. Two models were adopted, called Multi-Tester and Single-Tester. The genotypic values of the A-lines when crossed with tester T2, were positive for nine A-lines (12, 17, 6, 20, 18, 16, 19, 11 and 3) with emphasis on A-lines 12, 17 and 6, which were the highest values, respectively, the same tester for the trait FLOW, ten A-lines were positive values (3, 16, 17, 4, 12, 15, 5, 6, 8, 20) with emphasis on 3, 16 and 17. The genotypic values for TSS (Brix) trait of the A-lines when crossed with tester T1 were positive for A-lines (19, 18, 20, 16, 1, 5, and 6) with emphasis on lines 19, 18, 20, and 16, with the highest values, respectively. Only two A-lines showed positive genotypic values for three traits, A-line 16 and 20. These results indicate the need for continuing simultaneous improvement of A-lines for FLOW, FBY, and TSS. A-lines from the sorghum breeding program of Embrapa Milho e Sorgo have the potential for use as parents in the development of superior sweet sorghum hybrids for bioethanol production. The A-lines 6 and 20 have positive genotypic values for three traits, this A-lines has a potential for producing hybrids dedicated to bioethanol production. The T1-L tester was more efficient in discriminating the genetic values between the A lines for TSS, while the T2-L tester for FBY and FLOW. The development of a tester with genotype "drydry" and "Ma1Ma1" is important in order to develop superior hybrids to produce bioethanol involving the A, B and R scheme. MenosThere is a growing demand for the development of alternative fuels from renewable sources, thus sweet sorghum an autogamous species has potential for bioethanol production. Traditionally recommended cultivars were varieties, however, with the development of cytoplasmic male sterile sweet sorghum lines, sweet sorghum hybrids have become economically viable. The sweet sorghum breeding program of the Brazilian Agricultural Research Corporation (Embrapa Milho e Sorgo) has been developing sweet sorghum hybrids for bioethanol production according to the scheme of A, B and R-lines are used, where A-lines are used as females in the production of hybrid seeds. A and B-lines are isogenic, which differ only its cytoplasm source, sterile and normal, respectively, and fertility restorers R-lines. Thus the objective of this work was to evaluate the genetic potential of new male-sterile sweet sorghum A-lines using testcross hybrids developed with two sweet sorghum R-line as testers for agroindustry characteristics for bioethanol production. Forty testcross hybrids were developed using 20 experimental A-lines and two testers sweet sorghum R-lines. Experiments were conducted at experimental stations in Sete Lagoas-MG and Nova Porteirinha-MG, to evaluate 20 A-lines, two 2 R-lines, the resulting 40 testcross hybrids, and two controls. The 64 treatments were evaluated for days to flowering, fresh biomass yield and total soluble solids (Brix). The data obtained were analyzed using the ASReml pac... Mostrar Tudo |
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Palavras-Chave: |
Bioetanol. |
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Thesagro: |
Genética Vegetal; Sorghum Bicolor; Sorgo. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 03921naa a2200253 a 4500
001 2124208
005 2020-11-04
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.indcrop.2020.112577$2DOI
100 1 $aRIBEIRO, P. C. de O.
245 $aInsight into genetic potential of male sterile sweet sorghum A-lines for agroindustrial traits using tester R-lines.$h[electronic resource]
260 $c2020
520 $aThere is a growing demand for the development of alternative fuels from renewable sources, thus sweet sorghum an autogamous species has potential for bioethanol production. Traditionally recommended cultivars were varieties, however, with the development of cytoplasmic male sterile sweet sorghum lines, sweet sorghum hybrids have become economically viable. The sweet sorghum breeding program of the Brazilian Agricultural Research Corporation (Embrapa Milho e Sorgo) has been developing sweet sorghum hybrids for bioethanol production according to the scheme of A, B and R-lines are used, where A-lines are used as females in the production of hybrid seeds. A and B-lines are isogenic, which differ only its cytoplasm source, sterile and normal, respectively, and fertility restorers R-lines. Thus the objective of this work was to evaluate the genetic potential of new male-sterile sweet sorghum A-lines using testcross hybrids developed with two sweet sorghum R-line as testers for agroindustry characteristics for bioethanol production. Forty testcross hybrids were developed using 20 experimental A-lines and two testers sweet sorghum R-lines. Experiments were conducted at experimental stations in Sete Lagoas-MG and Nova Porteirinha-MG, to evaluate 20 A-lines, two 2 R-lines, the resulting 40 testcross hybrids, and two controls. The 64 treatments were evaluated for days to flowering, fresh biomass yield and total soluble solids (Brix). The data obtained were analyzed using the ASReml package, for the estimation of variance components and the prediction of genetic and non-genetic values via REML/BLUP. Two models were adopted, called Multi-Tester and Single-Tester. The genotypic values of the A-lines when crossed with tester T2, were positive for nine A-lines (12, 17, 6, 20, 18, 16, 19, 11 and 3) with emphasis on A-lines 12, 17 and 6, which were the highest values, respectively, the same tester for the trait FLOW, ten A-lines were positive values (3, 16, 17, 4, 12, 15, 5, 6, 8, 20) with emphasis on 3, 16 and 17. The genotypic values for TSS (Brix) trait of the A-lines when crossed with tester T1 were positive for A-lines (19, 18, 20, 16, 1, 5, and 6) with emphasis on lines 19, 18, 20, and 16, with the highest values, respectively. Only two A-lines showed positive genotypic values for three traits, A-line 16 and 20. These results indicate the need for continuing simultaneous improvement of A-lines for FLOW, FBY, and TSS. A-lines from the sorghum breeding program of Embrapa Milho e Sorgo have the potential for use as parents in the development of superior sweet sorghum hybrids for bioethanol production. The A-lines 6 and 20 have positive genotypic values for three traits, this A-lines has a potential for producing hybrids dedicated to bioethanol production. The T1-L tester was more efficient in discriminating the genetic values between the A lines for TSS, while the T2-L tester for FBY and FLOW. The development of a tester with genotype "drydry" and "Ma1Ma1" is important in order to develop superior hybrids to produce bioethanol involving the A, B and R scheme.
650 $aGenética Vegetal
650 $aSorghum Bicolor
650 $aSorgo
653 $aBioetanol
700 1 $aMARÇAL, T. de S.
700 1 $aOLIVEIRA, I. C. M.
700 1 $aSCHAFFERT, R. E.
700 1 $aCARNEIRO, P. C. S.
700 1 $aOLIVEIRA, A. B. de
700 1 $aPARRELLA, R. A. da C.
773 $tIndustrial Crops and Products$gv. 153, article 112557, 2020.
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