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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
05/09/2023 |
Data da última atualização: |
29/11/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BACANELLI, G.; ARAUJO, F. R.; VERBISCK, N. V. |
Afiliação: |
GISELE BACANELLI, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; FLABIO RIBEIRO DE ARAUJO, CNPGC; NEWTON VALERIO VERBISCK, CNPGC. |
Título: |
Improved MALDI-TOF MS identification of Mycobacterium tuberculosis by use of an enhanced cell disruption protocol. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Microorganisms, v. 11, issue 7, article 1692, 2023. |
DOI: |
https://doi.org/10.3390/ microorganisms11071692 |
Idioma: |
Inglês |
Notas: |
Communication. |
Conteúdo: |
ABSTRACT - Mycobacterium tuberculosis is the microorganism that causes tuberculosis, a disease affecting millions of people worldwide. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a fast, reliable, and cost-effective method for microorganism identification which has been used for the identification of Mycobacterium spp. isolates. However, the mycobacteria cell wall is rich in lipids, which makes it difficult to obtain proteins for MALDI-TOF MS analysis. In this study, two cell preparation protocols were compared: the MycoEx, recommended by MALDI-TOF instrument manufacturer Bruker Daltonics, and the MycoLyser protocol described herein, which used the MagNA Lyser instrument to enhance cell disruption with ethanol. Cell disruption and protein extraction steps with the two protocols were performed using the Mycobacterium tuberculosis H37Rv strain, and the MALDI-TOF MS results were compared. The MycoLyser protocol allowed for improved Biotyper identification of M. tuberculosis since the log(score) values obtained with this protocol were mostly > 1.800 and significantly higher than that underwent MycoEx processing. The identification reliability was increased as well, considering the Bruker criteria. In view of these results, it is concluded that the MycoLyser protocol for mycobacterial cell disruption and protein extraction improves the MALDI-TOF MS method's efficacy for M. tuberculosis identification. |
Palavras-Chave: |
MALDI-TOF MS. |
Thesagro: |
Espectrometria; Microrganismo; Tuberculose. |
Thesaurus Nal: |
Mass spectrometry; Microorganisms; Mycobacterium tuberculosis. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/255700/1/Improved-MALDI-TOF-MS-identification-2023.pdf
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Marc: |
LEADER 02272naa a2200253 a 4500 001 2156432 005 2023-11-29 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3390/ microorganisms11071692$2DOI 100 1 $aBACANELLI, G. 245 $aImproved MALDI-TOF MS identification of Mycobacterium tuberculosis by use of an enhanced cell disruption protocol.$h[electronic resource] 260 $c2023 500 $aCommunication. 520 $aABSTRACT - Mycobacterium tuberculosis is the microorganism that causes tuberculosis, a disease affecting millions of people worldwide. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a fast, reliable, and cost-effective method for microorganism identification which has been used for the identification of Mycobacterium spp. isolates. However, the mycobacteria cell wall is rich in lipids, which makes it difficult to obtain proteins for MALDI-TOF MS analysis. In this study, two cell preparation protocols were compared: the MycoEx, recommended by MALDI-TOF instrument manufacturer Bruker Daltonics, and the MycoLyser protocol described herein, which used the MagNA Lyser instrument to enhance cell disruption with ethanol. Cell disruption and protein extraction steps with the two protocols were performed using the Mycobacterium tuberculosis H37Rv strain, and the MALDI-TOF MS results were compared. The MycoLyser protocol allowed for improved Biotyper identification of M. tuberculosis since the log(score) values obtained with this protocol were mostly > 1.800 and significantly higher than that underwent MycoEx processing. The identification reliability was increased as well, considering the Bruker criteria. In view of these results, it is concluded that the MycoLyser protocol for mycobacterial cell disruption and protein extraction improves the MALDI-TOF MS method's efficacy for M. tuberculosis identification. 650 $aMass spectrometry 650 $aMicroorganisms 650 $aMycobacterium tuberculosis 650 $aEspectrometria 650 $aMicrorganismo 650 $aTuberculose 653 $aMALDI-TOF MS 700 1 $aARAUJO, F. R. 700 1 $aVERBISCK, N. V. 773 $tMicroorganisms$gv. 11, issue 7, article 1692, 2023.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroindústria Tropical. |
Data corrente: |
01/10/2004 |
Data da última atualização: |
05/08/2011 |
Autoria: |
SANTOS, A. A. dos; CARDOSO, J. E. |
Título: |
Número de aplicações de metalaxyl + mancozeb para o controle do míldio-do-meloeiro. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
Fortaleza: Embrapa Agroindustria Tropical, 2004. |
Páginas: |
3 p. |
Série: |
(Embrapa Agroindustria Tropical. Comunicado Tecnico, 92) |
Idioma: |
Português |
Conteúdo: |
Este trabalho teve o objetivo de determinar o numero de aplicacoes do fungicida metalaxyl + mancozeb, suficiente para o controle do mildio. |
Palavras-Chave: |
Controle; Cucumis melo L; Meloeiro; Mildio - Doenca. |
Thesagro: |
Controle Químico; Cucumis Melo; Doença; Doença de Planta; Doença Fúngica; Fungicida; Fungo; Melão; Míldio. |
Thesaurus NAL: |
mancozeb; metalaxyl; Pseudoperonospora cubensis. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPAT-2010/8611/1/Ct-092.pdf
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Marc: |
LEADER 01061nam a2200337 a 4500 001 1425647 005 2011-08-05 008 2004 bl uuuu u0uu1 u #d 100 1 $aSANTOS, A. A. dos 245 $aNúmero de aplicações de metalaxyl + mancozeb para o controle do míldio-do-meloeiro. 260 $aFortaleza: Embrapa Agroindustria Tropical$c2004 300 $a3 p. 490 $a(Embrapa Agroindustria Tropical. Comunicado Tecnico, 92) 520 $aEste trabalho teve o objetivo de determinar o numero de aplicacoes do fungicida metalaxyl + mancozeb, suficiente para o controle do mildio. 650 $amancozeb 650 $ametalaxyl 650 $aPseudoperonospora cubensis 650 $aControle Químico 650 $aCucumis Melo 650 $aDoença 650 $aDoença de Planta 650 $aDoença Fúngica 650 $aFungicida 650 $aFungo 650 $aMelão 650 $aMíldio 653 $aControle 653 $aCucumis melo L 653 $aMeloeiro 653 $aMildio - Doenca 700 1 $aCARDOSO, J. E.
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Embrapa Agroindústria Tropical (CNPAT) |
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