Data/hora: 23/06/2024 01:03:19

Biblioteca(s): | Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: | 15/12/2015
Data da última atualização: | 20/06/2024
Autoria: | BITTENCOURT, C. R.; FARIAS, E. A. de O.; BEZERRA, K. C.; VÉRAS, L. M. C.; Silva, V. C.; COSTA, C. H. N.; BEMQUERER, M. P.; SILVA, L. P. da; LEITE, J. R. de S. de A.; EIRAS, C.
Afiliação: | CLICIA RAMOS BITTENCOURT, UNIVERSIDADE FEDERAL DO PIAUÍ; EMANUEL AIRTON DE OLIVEIRA FARIAS, UNIVERSIDADE FEDERAL DO PIAUÍ; KARLA COSTA BEZERRA, UNIVERSIDADE FEDERAL DO PIAUÍ; LEIZ MARIA COSTA VÉRAS, UNIVERSIDADE FEDERAL DO PIAUÍ; VLADIMIR COSTA SILVA, UNIVERSIDADE FEDERAL DO PIAUÍ; CARLOS HENRIQUE NERY COSTA, INSTITUTO DE DOENÇAS TROPICAIS NATAN PORTELA–IDTNP; MARCELO PORTO BEMQUERER, CENARGEN; LUCIANO PAULINO DA SILVA, CENARGEN; JOSÉ ROBERTO DE SOUZA DE ALMEIDA LEITE, UNIVERSIDADE FEDERAL DO PIAUÍ; CARLA EIRAS, UNIVERSIDADE FEDERAL DO PIAUÍ.
Título: | Immobilization of cationic antimicrobial peptides and natural cashew gum in nanosheet systems for the investigation of anti-leishmanial activity.
Ano de publicação: | 2016
Fonte/Imprenta: | Materials Science & Engineering C, v. 59, p. 549-555, 2016.
Idioma: | Inglês
Conteúdo: | This report details the development of thin films containing an antimicrobial peptide, specifically, dermaseptin 01 (GLWSTIKQKGKEAAIAAA-KAAGQAALGAL-NH2, [DRS 01]), and a natural polysaccharide, for a novel application in detecting the presence of Leishmania cells and maintaining anti-leishmanial activity. The peptide DRS 01 was immobilized in conjunction with natural cashew gum (CG) onto an indium tin oxide (ITO) substrate using the Layer-by-Layer (LbL) deposition technique. The LbL film ITO/CG/DRS 01, containing DRS 01 as the outer layer,was capable of detecting the presence of Leishmania cells and acting as an anti-leishmanial system. Detection was performed using cyclic voltammetry (CV) in phosphate buffer (pH 7.2) in the presence of promastigote cells (0?107 cells/mL). The results showed a linear and inversely proportional relation between the concentration of Leishmania infantumprotozoan cells and the measured current values obtained for the films, which was attributed to the effect of peptide-induced lysis of the cell membrane, and resulted in freed residues thatwere adsorbed on the electrode surface.With this, the paper shows amethod using thin films with this newmaterial to demonstrate the anti-leishmanial activity in vitro models of carpet-like mechanisms.
Palavras-Chave: | Cashew gum; Dispositivo eletroquímico.
Thesaurus Nal: | antimicrobial peptides.
Categoria do assunto: | --
Registro original: | Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)
Exemplares: | CENARGEN - (36099-1) - UPC - AP - DD -  -  -  - DIS