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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
29/06/2021 |
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Data da última atualização: |
01/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AZEVEDO, B. T.; VERCESI FILHO, A. E.; GUTMANIS, G.; VERISSIMO, C. J.; KATIKI, L. M.; OKINO, C. H.; OLIVEIRA, M. C. de S.; GIGLIOTI, R. |
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Afiliação: |
BIANCA TAINA AZEVEDO, IZ; ANIBAL EUGENIO VERCESI FILHO, IZ; GUNTA GUTMANIS, IZ; CECÍLIA JOSE VERISSIMO, IZ; LUCIANA MORITA KATIKI, IZ; CINTIA HIROMI OKINO, CPPSE; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; RODRIGO GIGLIOTI, IZ. |
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Título: |
New sensitive methods for fraud detection in buffalo dairy products. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
International Dairy Journal, v.117, 105013, jun. 2021. |
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Páginas: |
9 p. |
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DOI: |
https://doi.org/10.1016/j.idairyj.2021.105013 |
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Idioma: |
Inglês |
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Conteúdo: |
The rising consumption of buffalo milk derivatives in recent years has highlighted the search for buffaloes in Brazilian livestock farming. These products have greater added value compared with common dairy milk products. Consequently, addition of variable amounts of cows' milk during manufacture of buffalo dairy products may occur, which constitutes fraud by product adulteration. Thus, the present study developed and standardised a DNA extraction protocol for application on different dairy derivatives and two methods based on real-time PCR for fraud identification: high-resolution melting (HRM) and rhAmp® SNP system. The extraction method allowed to extraction of DNA from eleven different dairy products. The sensitivity of the rhAmp method (1%) was five times higher than HRM assay (5%) and may be considered a better choice for identification of product adulteration when high sensitivity levels are required. |
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Palavras-Chave: |
DNA extraction; High resolution melting; Real time PCR; RhAmpR SNP system. |
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Thesaurus Nal: |
Buffalo milk. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01769naa a2200289 a 4500
001 2132629
005 2021-07-01
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.idairyj.2021.105013$2DOI
100 1 $aAZEVEDO, B. T.
245 $aNew sensitive methods for fraud detection in buffalo dairy products.$h[electronic resource]
260 $c2021
300 $a9 p.
520 $aThe rising consumption of buffalo milk derivatives in recent years has highlighted the search for buffaloes in Brazilian livestock farming. These products have greater added value compared with common dairy milk products. Consequently, addition of variable amounts of cows' milk during manufacture of buffalo dairy products may occur, which constitutes fraud by product adulteration. Thus, the present study developed and standardised a DNA extraction protocol for application on different dairy derivatives and two methods based on real-time PCR for fraud identification: high-resolution melting (HRM) and rhAmp® SNP system. The extraction method allowed to extraction of DNA from eleven different dairy products. The sensitivity of the rhAmp method (1%) was five times higher than HRM assay (5%) and may be considered a better choice for identification of product adulteration when high sensitivity levels are required.
650 $aBuffalo milk
653 $aDNA extraction
653 $aHigh resolution melting
653 $aReal time PCR
653 $aRhAmpR SNP system
700 1 $aVERCESI FILHO, A. E.
700 1 $aGUTMANIS, G.
700 1 $aVERISSIMO, C. J.
700 1 $aKATIKI, L. M.
700 1 $aOKINO, C. H.
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aGIGLIOTI, R.
773 $tInternational Dairy Journal$gv.117, 105013, jun. 2021.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
10/01/2017 |
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Data da última atualização: |
10/02/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
MATTOS-JR., D.; HIPPLER, F. W. R.; BOARETTO, R. M.; STUCHI, E. S.; QUAGGIO, J. A. |
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Afiliação: |
DIRCEU MATTOS-JR., Centro de Citricultura Sylvio Moreira; FRANZ W. R. HIPPLER, Centro de Citricultura Sylvio Moreira; RODRIGO M. BOARETTO, Centro de Citricultura Sylvio Moreira; EDUARDO SANCHES STUCHI, CNPMF; JOSÉ A. QUAGGIO, Centro de Solos e Recursos Ambientais. |
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Título: |
Soil boron fertilization: the role of nutrient sources and rootstocks in citrus production. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of Integrative Agriculture, v. 16, n.0, p. 60345-72017. |
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Idioma: |
Inglês |
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Conteúdo: |
Boron (B) is a key element for citrus production, especially in tropical regions, where the nutrient availability is commonly low in the soil. In addition, information about doses, fertilizer sources, methods of application and, particularly, differential nutrient demand of scion/rootstock combinations are required for efficient fertilization of commercial groves. In a non-irrigated sweet orange orchard (cv. Natal), grafted onto Rangpur lime, Swingle citrumelo or Sunki mandarin, we studied the application of two sources of B: boric acid (17% B, soluble in water) and ulexite (12% B, partially soluble in water) at four levels of supply (control without B, and soil application of 2, 4 and 6 kg ha?1 yr?1 of B). The experiment was carried out for three years. Boron availability in the soil and concentration in the leaves, as well as the fruit yield and quality of trees were evaluated. Soil B extracted with hot water and total leaf B positively correlated with doses of the nutrient applied to the trees. Levels of B in the soil and in the leaves did not vary with fertilizer sources. Fruit yield of trees grafted onto Rangpur lime and Swingle citrumelo was more responsive to B doses than those grafted onto Sunki mandarin. Maximum fruit yield of trees grafted onto Swingle was obtained with 3.2 kg ha?1 yr?1 of B, and leaf B level of 280 mg kg?1 what point out to a highest demand for B when this combination was compared with other rootstocks. Furthermore, fertilization with B did not affect the quality of fruits, but correlated with B and potassium (K) concentrations in the leaves. These results also support that the current recommendations for levels of B in leaves should be revisited. MenosBoron (B) is a key element for citrus production, especially in tropical regions, where the nutrient availability is commonly low in the soil. In addition, information about doses, fertilizer sources, methods of application and, particularly, differential nutrient demand of scion/rootstock combinations are required for efficient fertilization of commercial groves. In a non-irrigated sweet orange orchard (cv. Natal), grafted onto Rangpur lime, Swingle citrumelo or Sunki mandarin, we studied the application of two sources of B: boric acid (17% B, soluble in water) and ulexite (12% B, partially soluble in water) at four levels of supply (control without B, and soil application of 2, 4 and 6 kg ha?1 yr?1 of B). The experiment was carried out for three years. Boron availability in the soil and concentration in the leaves, as well as the fruit yield and quality of trees were evaluated. Soil B extracted with hot water and total leaf B positively correlated with doses of the nutrient applied to the trees. Levels of B in the soil and in the leaves did not vary with fertilizer sources. Fruit yield of trees grafted onto Rangpur lime and Swingle citrumelo was more responsive to B doses than those grafted onto Sunki mandarin. Maximum fruit yield of trees grafted onto Swingle was obtained with 3.2 kg ha?1 yr?1 of B, and leaf B level of 280 mg kg?1 what point out to a highest demand for B when this combination was compared with other rootstocks. Furthermore, fertilization with B did not af... Mostrar Tudo |
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Thesaurus NAL: |
soil. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02253naa a2200181 a 4500
001 2060328
005 2017-02-10
008 2017 bl uuuu u00u1 u #d
100 1 $aMATTOS-JR., D.
245 $aSoil boron fertilization$bthe role of nutrient sources and rootstocks in citrus production.$h[electronic resource]
260 $c2017
520 $aBoron (B) is a key element for citrus production, especially in tropical regions, where the nutrient availability is commonly low in the soil. In addition, information about doses, fertilizer sources, methods of application and, particularly, differential nutrient demand of scion/rootstock combinations are required for efficient fertilization of commercial groves. In a non-irrigated sweet orange orchard (cv. Natal), grafted onto Rangpur lime, Swingle citrumelo or Sunki mandarin, we studied the application of two sources of B: boric acid (17% B, soluble in water) and ulexite (12% B, partially soluble in water) at four levels of supply (control without B, and soil application of 2, 4 and 6 kg ha?1 yr?1 of B). The experiment was carried out for three years. Boron availability in the soil and concentration in the leaves, as well as the fruit yield and quality of trees were evaluated. Soil B extracted with hot water and total leaf B positively correlated with doses of the nutrient applied to the trees. Levels of B in the soil and in the leaves did not vary with fertilizer sources. Fruit yield of trees grafted onto Rangpur lime and Swingle citrumelo was more responsive to B doses than those grafted onto Sunki mandarin. Maximum fruit yield of trees grafted onto Swingle was obtained with 3.2 kg ha?1 yr?1 of B, and leaf B level of 280 mg kg?1 what point out to a highest demand for B when this combination was compared with other rootstocks. Furthermore, fertilization with B did not affect the quality of fruits, but correlated with B and potassium (K) concentrations in the leaves. These results also support that the current recommendations for levels of B in leaves should be revisited.
650 $asoil
700 1 $aHIPPLER, F. W. R.
700 1 $aBOARETTO, R. M.
700 1 $aSTUCHI, E. S.
700 1 $aQUAGGIO, J. A.
773 $tJournal of Integrative Agriculture$gv. 16, n.0, p. 60345-72017.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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