Data/hora: 23/09/2024 23:34:11

Biblioteca(s): 	Embrapa Pantanal.	
Data corrente: 	15/07/2002	
Data da última atualização: 	30/03/2017	
Autoria: 	VENTURA, R. M.; TAKATA, C. S. A.; SILVA, R. A. M. S.; NUNES, V. L.; TAKEDA, G. F.; TEIXEIRA, M. M. G.	
Afiliação: 	USP/DP/ICB (Sao Paulo, SP). EMBRAPA Suinos e Aves (Concordia, SC).	
Título: 	Molecular and morphological studies of Brazilian Trypanosoma evansi stocks: the total absence of kDNA in Trypanosomes from both laboratory stocks and naturally infected domestic and wild mammals.	
Ano de publicação: 	2000	
Fonte/Imprenta: 	Journal of Parasitology, v.86, n.6, p.1289-1298, Dec. 2000.	
Idioma: 	Inglês	
Conteúdo: 	The kinetoplast DNA (kDNA) minircle molecules of 14 Brazilian stocks of Trypanosoma evansi were studied by morphological approaches (Giemsa and 4'-6'-diamidino-2-phenylin-
dole staining and transmission electyron microscopy) and molecular approaches (probing with an oligonucleotide complementary to the minicircle origin of replication and polymerase chain reaction amplification of a minicircle sequence). All methods indicated the absense of both a typical kinetoplast and kDNA minicircles, even in a very small number of parasites of a single stock or in small numbers of copies of molecules per cell. We did not detect any altered kDNA molecules. There were no KDNA molecules in eithger old or new
stocks of T. evansi maintained by successive passages in mice. Similarly, no kDNA minicircles were detected in trypanosomes in blood smears from naturally infected domestic and wild animals. Thus, the total absence of KDNA in Brazilian stocks of T. evansi from both domestic and wild mammals is probably the natural state of Brazilian T. evansi.	
Palavras-Chave: 	Domestic animal; Infeccao natural; Mamifero silvestre; Molecular; Morfologia; Morphology; Naturally infected; Parasita; Parasite; Wild mammal.	
Thesagro: 	Animal Domestico.	
Thesaurus Nal: 	Brazil; Trypanosoma evansi.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Pantanal (CPAP)	
Exemplares: 	CPAP - (52831-1) - ADD - SP - PP - SP16393 -  - 16393 - DIS