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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
09/01/2019 |
Data da última atualização: |
12/02/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
VASCONCELOS, M. J. V. de; ANTUNES, M. S.; OLIVEIRA, M. F. de; LOPES, M. A.; FIGUEIREDO, J. E. F. |
Afiliação: |
MARIA JOSE VILACA DE VASCONCELOS, CNPMS; MAURICIO SCHUSTERSCHITZ ANTUNES, Colorado State University; MAURILIO FERNANDES DE OLIVEIRA, CNPMS; MAURICIO ANTONIO LOPES, DE/PR; JOSE EDSON FONTES FIGUEIREDO, CNPMS. |
Título: |
Callus induction and plant regeneration from immature embryos culture of tropical maize. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 17, n. 3, p. 359-368, 2018. |
Idioma: |
Inglês |
Conteúdo: |
The development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that the problem of the recalcitrant genotypes can be, at least partially, overcome by using immature embryos as explants together with tissue culture media formulations. Keywords: Zea mays, immature embryos, somatic embryogenesis, tissue culture, type I and type II calli. MenosThe development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that ... Mostrar Tudo |
Palavras-Chave: |
Calos tipo I e tipo II; Embriogênese somática. |
Thesagro: |
Cultura de Tecido; Embrião Vegetal; Zea Mays. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/190064/1/Callus-induction.pdf
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Marc: |
LEADER 02500naa a2200229 a 4500 001 2103395 005 2019-02-12 008 2018 bl uuuu u00u1 u #d 100 1 $aVASCONCELOS, M. J. V. de 245 $aCallus induction and plant regeneration from immature embryos culture of tropical maize.$h[electronic resource] 260 $c2018 520 $aThe development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that the problem of the recalcitrant genotypes can be, at least partially, overcome by using immature embryos as explants together with tissue culture media formulations. Keywords: Zea mays, immature embryos, somatic embryogenesis, tissue culture, type I and type II calli. 650 $aCultura de Tecido 650 $aEmbrião Vegetal 650 $aZea Mays 653 $aCalos tipo I e tipo II 653 $aEmbriogênese somática 700 1 $aANTUNES, M. S. 700 1 $aOLIVEIRA, M. F. de 700 1 $aLOPES, M. A. 700 1 $aFIGUEIREDO, J. E. F. 773 $tRevista Brasileira de Milho e Sorgo, Sete Lagoas$gv. 17, n. 3, p. 359-368, 2018.
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Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
15/10/2021 |
Data da última atualização: |
15/10/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 3 |
Autoria: |
SILVA, P. C.; RIBEIRO JUNIOR, W. Q.; RAMOS, M. L. G.; CELESTINO, S. M. C.; SILVA, A. N.; CASARI, R. A. C. N.; SANTANA, C. C.; LIMA, C. A. de; WILLIAMS, T. C. R.; VINSON, C. C. |
Afiliação: |
PATRÍCIA CARVALHO DA SILVA; WALTER QUADROS RIBEIRO JUNIOR, CPAC; MARIA LUCRECIA GEROSA RAMOS; SONIA MARIA COSTA CELESTINO, CPAC; ALBERTO DO NASCIMENTO SILVA; RAPHAEL AUGUSTO DAS CHAGAS NOQUELI CASARI; CHARLES CARDOSO SANTANA; CRISTIANE ANDREA DE LIMA; THOMAS CHRISTOPHER RHYS WILLIAMS; CHRISTINA CLEO VINSON. |
Título: |
Quinoa for the Brazilian Cerrado: Agronomic Characteristics of Elite Genotypes under Different Water Regimes. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Plants, v. 10, n. 1591, 2021. |
Páginas: |
17 p. |
Idioma: |
Português |
Conteúdo: |
Abstract: Quinoa stands out as an excellent crop in the Cerrado region for cultivation in the offseason or irrigated winter season. Here, we tested the effects of different water regimes on the agronomic characteristics, physiology, and grain quality of different elite quinoa genotypes under field conditions. The experiment was conducted under field conditions at Embrapa Cerrados (Planaltina, DF, Brazil). The experimental design was in randomized blocks, in a split-plot scheme, with four replications. The plots were composed of 18 quinoa genotypes and modified BRS Piabiru (the currently used genotype), and the split-plots were divided into 4 different water regimes. The following variables were evaluated: productivity and productivity per unit of applied water (PUAA), plant height, flavonoids, anthocyanins, gas exchange, chlorophyll, leaf proline, and relative water content. Our results showed that water regimes between 309 and 389 mm can be recommended for quinoa in the Cerrado region. CPAC6 and CPAC13 presented the highest yield and PUAA under high and intermediate WRs, and hence were the most suitable for winter growth under irrigation. CPAC17 is most suitable for off-season growth under rainfed conditions, as it presented the highest PUAA under the low WRs (247 and 150). CPAC9 stood out in terms of accumulation of flavonoids and anthocyanins in all WRs. Physiological analyses revealed different responses of the genotypes to water restriction, together with symptoms of stress under lower water regimes. Our study reinforces the importance of detailed analyses of the relationship between productivity, physiology, and water use when choosing genotypes for planting and harvest in different seasons. MenosAbstract: Quinoa stands out as an excellent crop in the Cerrado region for cultivation in the offseason or irrigated winter season. Here, we tested the effects of different water regimes on the agronomic characteristics, physiology, and grain quality of different elite quinoa genotypes under field conditions. The experiment was conducted under field conditions at Embrapa Cerrados (Planaltina, DF, Brazil). The experimental design was in randomized blocks, in a split-plot scheme, with four replications. The plots were composed of 18 quinoa genotypes and modified BRS Piabiru (the currently used genotype), and the split-plots were divided into 4 different water regimes. The following variables were evaluated: productivity and productivity per unit of applied water (PUAA), plant height, flavonoids, anthocyanins, gas exchange, chlorophyll, leaf proline, and relative water content. Our results showed that water regimes between 309 and 389 mm can be recommended for quinoa in the Cerrado region. CPAC6 and CPAC13 presented the highest yield and PUAA under high and intermediate WRs, and hence were the most suitable for winter growth under irrigation. CPAC17 is most suitable for off-season growth under rainfed conditions, as it presented the highest PUAA under the low WRs (247 and 150). CPAC9 stood out in terms of accumulation of flavonoids and anthocyanins in all WRs. Physiological analyses revealed different responses of the genotypes to water restriction, together with symptoms of st... Mostrar Tudo |
Palavras-Chave: |
Eficiência do uso da água; Troca gasosa. |
Thesagro: |
Chenopodium Quinoa; Composto Fenólico. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226981/1/Quinoa-for-the-Brazilian-Cerrado-Walter.pdf
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Marc: |
LEADER 02573naa a2200289 a 4500 001 2135368 005 2021-10-15 008 2021 bl uuuu u00u1 u #d 100 1 $aSILVA, P. C. 245 $aQuinoa for the Brazilian Cerrado$bAgronomic Characteristics of Elite Genotypes under Different Water Regimes.$h[electronic resource] 260 $c2021 300 $a17 p. 520 $aAbstract: Quinoa stands out as an excellent crop in the Cerrado region for cultivation in the offseason or irrigated winter season. Here, we tested the effects of different water regimes on the agronomic characteristics, physiology, and grain quality of different elite quinoa genotypes under field conditions. The experiment was conducted under field conditions at Embrapa Cerrados (Planaltina, DF, Brazil). The experimental design was in randomized blocks, in a split-plot scheme, with four replications. The plots were composed of 18 quinoa genotypes and modified BRS Piabiru (the currently used genotype), and the split-plots were divided into 4 different water regimes. The following variables were evaluated: productivity and productivity per unit of applied water (PUAA), plant height, flavonoids, anthocyanins, gas exchange, chlorophyll, leaf proline, and relative water content. Our results showed that water regimes between 309 and 389 mm can be recommended for quinoa in the Cerrado region. CPAC6 and CPAC13 presented the highest yield and PUAA under high and intermediate WRs, and hence were the most suitable for winter growth under irrigation. CPAC17 is most suitable for off-season growth under rainfed conditions, as it presented the highest PUAA under the low WRs (247 and 150). CPAC9 stood out in terms of accumulation of flavonoids and anthocyanins in all WRs. Physiological analyses revealed different responses of the genotypes to water restriction, together with symptoms of stress under lower water regimes. Our study reinforces the importance of detailed analyses of the relationship between productivity, physiology, and water use when choosing genotypes for planting and harvest in different seasons. 650 $aChenopodium Quinoa 650 $aComposto Fenólico 653 $aEficiência do uso da água 653 $aTroca gasosa 700 1 $aRIBEIRO JUNIOR, W. Q. 700 1 $aRAMOS, M. L. G. 700 1 $aCELESTINO, S. M. C. 700 1 $aSILVA, A. N. 700 1 $aCASARI, R. A. C. N. 700 1 $aSANTANA, C. C. 700 1 $aLIMA, C. A. de 700 1 $aWILLIAMS, T. C. R. 700 1 $aVINSON, C. C. 773 $tPlants$gv. 10, n. 1591, 2021.
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