Data/hora: 19/06/2024 12:53:51

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 15/04/2016
Data da última atualização: | 15/04/2016
Autoria: | ALVES, N. E. G.; VASCONCELOS, C. M.; BASSINELLO, P. Z.; MEJIA, E. G. de; MARTINO, H. S. D.
Afiliação: | NATALIA ELIZABETH GALDINO ALVES, UFV; CHRISTIANE MILEIB VASCONCELOS, UNIVERSIDADE FEDERAL DE OURO PRETO; PRISCILA ZACZUK BASSINELLO, CNPAF; ELVIRA GONZALEZ DE MEJIA, UNIVERSITY OF ILLINOIS URBANA, Champaign; HERCIA STAMPINI DUARTE MARTINO, UFV.
Título: | Digested protein isolate from fresh and stored Carioca beans reduced markers of atherosclerosis in oxidized LDL-induced THP-1 macrophages.
Ano de publicação: | 2016
Fonte/Imprenta: | Journal of Functional Foods, v. 24, p. 97-111, 2016.
ISSN: | 1756-4646
DOI: | 10.1016/j.jff.2016.03.027
Idioma: | Inglês
Conteúdo: | Oxidized LDL (ox-LDL) and its interaction with lectin type ox-LDL receptor (LOX-1) determine atherosclerosis progression. The potential of peptides from digested protein isolate (DPI) derived from fresh and stored Carioca bean (Phaseolus vulgaris L.) to prevent ox-LDL induced inflammation in humanTHP-1 macrophage-like cells was examined here. BRS Pontal (PO) and BRSMG Madreperola (MP) fresh and stored for 6 months were cooked, and protein isolates were digested by pepsin?pancreatin. Peptides from stored Carioca beans decreased prostaglandin E-2 (PGE-2) (from 17.7 to 47%) and tumour necrosis factor-&#945; (TNF-&#945;) (12.3 to 82%). Stored MP (0.01 mg/mL) inhibited reactive oxygen species (ROS). LOX-1 and matrix metalloproteinase-9 (MMP-9) expression were reduced by peptides from all beans. Stored PO and MP digested protein isolates reduced expression of 10 cytokines related to atherosclerosis process. Peptides from Carioca beans presented anti-atherosclerotic properties comparable to simvastatin, which were related to inhibition of LOX-1, MMP-9 and ICAM-1.
Palavras-Chave: | Bioactive peptides; Lectin type ox-LDL receptor 1; Oxidized LDL.
Thesagro: | Feijão; Phaseolus vulgaris.
Categoria do assunto: | Q Alimentos e Nutrição Humana
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (34146-1) - UPC - AP - DD - 2016 -  - 2016 - DIS