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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
15/12/2015 |
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Data da última atualização: |
20/06/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARCANJO, D. D. R.; VASCONCELOS, A. G.; COMERMA-STEFFENSEN, S. G.; JESUS, J. R.; SILVA, L. P. da; PIRES JÚNIOR, O. R.; COSTA-NETO, C. M.; OLIVEIRA, E. B.; MIGLIOLO, L.; FRANCO, O. L.; RESTINI, C. B. A.; PAULO, M.; BENDHACK, L. M.; BEMQUERER, M. P.; OLIVEIRA, A. P.; SIMONSEN, U.; LEITE, J. R. de S. de A. |
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Afiliação: |
DANIEL DIAS RUFINO ARCANJO, UNIVERSIDADE FEDERAL DO PIAUÍ; ANDREANNE GOMES VASCONCELOS, UNIVERSIDADE FEDERAL DO PIAUÍ; SIMÓN GABRIEL, AARHUS UNIVERSITY; JOILSON RAMOS JESUS, UNIVERSIDADE FEDERAL DO PIAUÍ; LUCIANO PAULINO DA SILVA, CENARGEN; OSMINDO RODRIGUES PIRES JÚNIOR, UNIVERSIDADE DE BRASÍLIA; CLAUDIO MIGUEL COSTA-NETO, UNIVERSIDADE DE SÃO PAULO; EDUARDO BRANDT OLIVEIRA, UNIVERSIDADE DE SÃO PAULO; LUDOVICO MIGLIOLO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; OCTÁVIO LUIZ FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; CAROLINA BARALDI ARAÚJO RESTINI, UNIVERSIDADE DE RIBEIRÃO PRETO; MICHELE PAULO, UNIVERSIDADE DE SÃO PAULO; LUSIANE MARIA BENDHACK, UNIVERSIDADE DE SÃO PAULO; MARCELO PORTO BEMQUERER, CENARGEN; ALDEIDIA PEREIRA OLIVEIRA, UNIVERSIDADE FEDERAL DO PIAUÍ; ULF SIMONSEN, AARHUS UNIVERSITY; JOSÉ ROBERTO DE SOUZA DE ALMEIDA LEITE, UNIVERSIDADE FEDERAL DO PIAUÍ. |
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Título: |
A novel vasoactive proline-rich oligopeptide from the skin secretion of the frog Brachycephalus ephippium. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plos One, v. 10, dez. 2015. (Open Access) |
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DOI: |
10.1371/journal.pone.0145071 |
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Idioma: |
Inglês |
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Conteúdo: |
Proline-rich oligopeptides (PROs) are a large family which comprises the bradykinin-potentiating peptides (BPPs). They inhibit the activity of the angiotensin I-converting enzyme (ACE) and have a typical pyroglutamyl (Pyr)/proline-rich structure at the N- and C-terminus, respectively. Furthermore, PROs decrease blood pressure in animals. In the present study, the isolation and biological characterization of a novel vasoactive BPP isolated from the skin secretion of the frog Brachycephalus ephippium is described. This new PRO, termed BPP-Brachy, has the primary structure WPPPKVSP and the amidated form termed BPPBrachyNH2 inhibits efficiently ACE in rat serum. In silico molecular modeling and docking studies suggest that BPP-BrachyNH2 is capable of forming a hydrogen bond network as well as multiple van der Waals interactions with the rat ACE, which blocks the access of the substrate to the C-domain active site. Moreover, in rat thoracic aorta BPP-BrachyNH2 induces potent endothelium-dependent vasodilatation with similar magnitude as captopril. In DAF-FM DA-loaded aortic cross sections examined by confocal microscopy, BPP-BrachyNH2 was found to increase the release of nitric oxide (NO). Moreover, BPP-BrachyNH2 was devoid of toxicity in endothelial and smooth muscle cell cultures. In conclusion, the peptide BPP-BrachyNH2 has a novel sequence being the first BPP isolated from the skin secretion of the Brachycephalidae family. This opens for exploring amphibians as a source of new biomolecules. The BPP-BrachyNH2 is devoid of cytotoxicity and elicits endothelium-dependent vasodilatation mediated by NO. These findings open for the possibility of potential application of these peptides in the treatment of endothelial dysfunction and cardiovascular diseases. MenosProline-rich oligopeptides (PROs) are a large family which comprises the bradykinin-potentiating peptides (BPPs). They inhibit the activity of the angiotensin I-converting enzyme (ACE) and have a typical pyroglutamyl (Pyr)/proline-rich structure at the N- and C-terminus, respectively. Furthermore, PROs decrease blood pressure in animals. In the present study, the isolation and biological characterization of a novel vasoactive BPP isolated from the skin secretion of the frog Brachycephalus ephippium is described. This new PRO, termed BPP-Brachy, has the primary structure WPPPKVSP and the amidated form termed BPPBrachyNH2 inhibits efficiently ACE in rat serum. In silico molecular modeling and docking studies suggest that BPP-BrachyNH2 is capable of forming a hydrogen bond network as well as multiple van der Waals interactions with the rat ACE, which blocks the access of the substrate to the C-domain active site. Moreover, in rat thoracic aorta BPP-BrachyNH2 induces potent endothelium-dependent vasodilatation with similar magnitude as captopril. In DAF-FM DA-loaded aortic cross sections examined by confocal microscopy, BPP-BrachyNH2 was found to increase the release of nitric oxide (NO). Moreover, BPP-BrachyNH2 was devoid of toxicity in endothelial and smooth muscle cell cultures. In conclusion, the peptide BPP-BrachyNH2 has a novel sequence being the first BPP isolated from the skin secretion of the Brachycephalidae family. This opens for exploring amphibians as a source of ne... Mostrar Tudo |
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Palavras-Chave: |
Brachycephalus ephippium; Proline-rich oligopeptides. |
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Thesaurus Nal: |
secretion. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182309/1/journal.pone.0145071.PDF
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Marc: |
LEADER 02864naa a2200361 a 4500
001 2031700
005 2024-06-20
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0145071$2DOI
100 1 $aARCANJO, D. D. R.
245 $aA novel vasoactive proline-rich oligopeptide from the skin secretion of the frog Brachycephalus ephippium.$h[electronic resource]
260 $c2015
520 $aProline-rich oligopeptides (PROs) are a large family which comprises the bradykinin-potentiating peptides (BPPs). They inhibit the activity of the angiotensin I-converting enzyme (ACE) and have a typical pyroglutamyl (Pyr)/proline-rich structure at the N- and C-terminus, respectively. Furthermore, PROs decrease blood pressure in animals. In the present study, the isolation and biological characterization of a novel vasoactive BPP isolated from the skin secretion of the frog Brachycephalus ephippium is described. This new PRO, termed BPP-Brachy, has the primary structure WPPPKVSP and the amidated form termed BPPBrachyNH2 inhibits efficiently ACE in rat serum. In silico molecular modeling and docking studies suggest that BPP-BrachyNH2 is capable of forming a hydrogen bond network as well as multiple van der Waals interactions with the rat ACE, which blocks the access of the substrate to the C-domain active site. Moreover, in rat thoracic aorta BPP-BrachyNH2 induces potent endothelium-dependent vasodilatation with similar magnitude as captopril. In DAF-FM DA-loaded aortic cross sections examined by confocal microscopy, BPP-BrachyNH2 was found to increase the release of nitric oxide (NO). Moreover, BPP-BrachyNH2 was devoid of toxicity in endothelial and smooth muscle cell cultures. In conclusion, the peptide BPP-BrachyNH2 has a novel sequence being the first BPP isolated from the skin secretion of the Brachycephalidae family. This opens for exploring amphibians as a source of new biomolecules. The BPP-BrachyNH2 is devoid of cytotoxicity and elicits endothelium-dependent vasodilatation mediated by NO. These findings open for the possibility of potential application of these peptides in the treatment of endothelial dysfunction and cardiovascular diseases.
650 $asecretion
653 $aBrachycephalus ephippium
653 $aProline-rich oligopeptides
700 1 $aVASCONCELOS, A. G.
700 1 $aCOMERMA-STEFFENSEN, S. G.
700 1 $aJESUS, J. R.
700 1 $aSILVA, L. P. da
700 1 $aPIRES JÚNIOR, O. R.
700 1 $aCOSTA-NETO, C. M.
700 1 $aOLIVEIRA, E. B.
700 1 $aMIGLIOLO, L.
700 1 $aFRANCO, O. L.
700 1 $aRESTINI, C. B. A.
700 1 $aPAULO, M.
700 1 $aBENDHACK, L. M.
700 1 $aBEMQUERER, M. P.
700 1 $aOLIVEIRA, A. P.
700 1 $aSIMONSEN, U.
700 1 $aLEITE, J. R. de S. de A.
773 $tPlos One$gv. 10, dez. 2015. (Open Access)
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 8 | |
| 1. |  | VASCONCELOS, A. G. V de; WILLADINO, L.; CAMARA, T. R.; TORRES, A. C.; LIRA, M. de A.; CAVALCANTI, V. A. L. B. Micropropagação da palma forrageira cv. Miúda. ABCTP Notícias, Brasília, n. 42, p.4-7, abr. 2002.| Biblioteca(s): Embrapa Hortaliças. |
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| 2. | | BRAND, G. D.; RAMADA, M. H. S.; MANICKCHAND, J. R.; CORREA, R.; RIBEIRO, D. J. S.; SANTOS, M. A.; VASCONCELOS, A. G.; ABRÃO, F. Y.; PRATES, M. V.; MURAD, A. M.; CARDOZO FILHO, J. L.; LEITE, R. S. A.; MAGALHÃES, K. G.; OLIVEIRA, A. L.; BLOCH JUNIOR, C. Intragenic antimicrobial peptides (IAPs) from human proteins with potent antimicrobial and anti-inflammatory activity. PLoS ONE, v.14, n. 8, article e0220656, 2019. Na publicação: José L. Cardozo Fh, Carlos Bloch, Jr.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 3. | | ARCANJO, D. D. R.; VASCONCELOS, A. G.; COMERMA-STEFFENSEN, S. G.; JESUS, J. R.; SILVA, L. P. da; PIRES JÚNIOR, O. R.; COSTA-NETO, C. M.; OLIVEIRA, E. B.; MIGLIOLO, L.; FRANCO, O. L.; RESTINI, C. B. A.; PAULO, M.; BENDHACK, L. M.; BEMQUERER, M. P.; OLIVEIRA, A. P.; SIMONSEN, U.; LEITE, J. R. de S. de A. A novel vasoactive proline-rich oligopeptide from the skin secretion of the frog Brachycephalus ephippium. Plos One, v. 10, dez. 2015. (Open Access)| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 4. | | SANTOS, M. A.; SILVA, F. L.; LIRA, B. O. V.; CARDOZO FILHO, J. L.; VASCONCELOS, A. G.; ARAUJO, A. R.; MURAD, A. M.; GARAY, A. V.; FREITAS, S. M.; LEITE, J. R. S. A.; BLOCH JUNIOR, C.; RAMADA, M. H. S.; OLIVEIRA, A. L. de; BRAND, G. D. Probing human proteins for short encrypted antimicrobial peptides reveals Hs10, a peptide with selective activity for gram-negative bacteria. Biochimica et Biophysica Acta - General Subjects, v. 1867, 2023. 130265. Na publicação: José L. Cardozo Fh; Carlos Bloch Jr.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | FREITAS, T. V. de; KARMAKAR, U.; VASCONCELOS, A. G.; SANTOS, M. A.; LIRA, B. O. do V.; COSTA, S. R.; BARBOSA, E. A.; CARDOZO FILHO, J.; CORREA, R.; RIBEIRO, D. J. S.; PRATES, M. V.; MAGALHÃES, K. G.; RAMADA, M. H. S.; LEITE, J. R. de S. A.; BLOCH JUNIOR, C.; OLIVEIRA, A. L. de; VENDRELL, M.; BRAND, G. D. Release of immunomodulatory peptides at bacterial membrane interfaces as a novel strategy to fight microorganisms. Journal of Biological Chemistry, v. 299, n. 4, 2023. 103056. Na publicação: José Cardozo-Fh; Carlos Bloch Jr.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | ARCANJO, D. D. R.; VASCONCELOS, A. G.; NASCIMENTO, L. A.; MAFUD, A. C.; PLÁCIDO, A.; ALVES, M. M. M.; DELERUE-MATOS, C.; BEMQUERER, M. P.; VALE, N.; GOMES, P.; OLIVEIRA, E. B.; LIMA, F. C. A.; MASCARENHAS, Y. P.; CARVALHO, F. A. A.; SIMONSEN, U.; RAMOS, R. M.; LEITE, J. R. S. A. Structure-function studies of BPP-BrachyNH2 and synthetic analogues thereof with Angiotensin I-Converting Enzyme. European Journal of Medicinal Chemistry, v.139, p. 401-411, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | ARCANJO, D. D. R.; MAFUD, A. C.; VASCONCELOS, A. G.; SILVA-FILHO, J. C. da; AMARAL, M. P. M.; BRITO, L. M.; BEMQUERER, M. P.; KÜCKELHAUS, S. A. S.; PLÁCIDO, A. P.; DELERUE-MATOS, C.; VALE, N.; MASCARENHAS, Y. P.; CARVALHO, F. A. A.; OLIVEIRA, A. P.; LEITE, J. R. S. A. In silico, in vitro and in vivo toxicological assessment of BPP-BrachyNH2, A vasoactive proline-rich oligopeptide from Brachycephalus ephippium. International Journal of Peptide Research and Therapeutics, v. 23, n. 3, p 323-331, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | SOUSA, N. A. de; MARANI, M. M.; LOPES, A. L. F.; SILVA, E. M.; BARBOSA, E. A.; VASCONCELOS, A. G.; KUZNIEWSKI, F. T. B.; LUSTOSA, S. S.; GOMES, K. P.; COLUGNATI, D. B.; ROCHA, J. A.; SANTOS, L. H.; BEMQUERER, M. P.; QUELEMES, P.; VÉRAS, L.; MOREIRA, D. C.; GADELHA, K. K. L.; MAGALHÃES, P. J. C.; PLÁCIDO, A.; EATON, E.; NICOLAU, L.; MEDEIROS, J. V. R.; LEITE, J. R. S. A. BR-bombesin: a novel bombesin-related peptide from the skin secretion of the Chaco tree frog (Boana raniceps) with physiological gastric effects. Amino Acids, v. 54, p. 733-747, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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