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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
08/05/2013 |
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Data da última atualização: |
13/03/2024 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SANTOS, A. S. DE O; JESUS, D. C.; MEURER, V. M.; VASCONCELOS, A. S. do E.; FURTADO, M. A. M.; MARTINS, M. F. |
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Afiliação: |
ALESSA SIQUEIRA DE O. SANTOS, UFJF; DEBORA CRISTINA JESUS, UFPR; VANEIDA MARIA MEURER, UFJF; ANTONIO SILVIO DO EGITO VASCONCELOS, CNPC; MARCO ANTONIO MOREIRA FURTADO, UFJF; MARTA FONSECA MARTINS, CNPGL. |
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Título: |
Quantificação das proteinas totais pela espectrofotometria UV: método de bradford em leite caprino adulterado com leite bovino. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: CONGRESSO LATINOAMERICANO, 6.; CONGRESSO BRASILEIRO DE HIGIENISTAS DE ALIMENTOS, 12., 2013, Gramado. Anais... Gramado: Colégio Brasileiro de Médicos Veterinários Higienistas de Alimentos, 2013. |
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Páginas: |
p. 2100-2104. |
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Idioma: |
Português |
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Notas: |
Publicado na revista Higiene Alimentar, v. 27, n. 218-219, 2013. |
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Palavras-Chave: |
Analise de proteinas; Espectrofotometria UV; Metodo de bradford. |
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Thesagro: |
Leite. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/957441/1/Quantificacao-das-proteinas-totais.pdf
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Marc: |
LEADER 00951nam a2200229 a 4500
001 1957441
005 2024-03-13
008 2013 bl uuuu u00u1 u #d
100 1 $aSANTOS, A. S. DE O
245 $aQuantificação das proteinas totais pela espectrofotometria UV$bmétodo de bradford em leite caprino adulterado com leite bovino.$h[electronic resource]
260 $aIn: CONGRESSO LATINOAMERICANO, 6.; CONGRESSO BRASILEIRO DE HIGIENISTAS DE ALIMENTOS, 12., 2013, Gramado. Anais... Gramado: Colégio Brasileiro de Médicos Veterinários Higienistas de Alimentos$c2013
300 $ap. 2100-2104.
500 $aPublicado na revista Higiene Alimentar, v. 27, n. 218-219, 2013.
650 $aLeite
653 $aAnalise de proteinas
653 $aEspectrofotometria UV
653 $aMetodo de bradford
700 1 $aJESUS, D. C.
700 1 $aMEURER, V. M.
700 1 $aVASCONCELOS, A. S. do E.
700 1 $aFURTADO, M. A. M.
700 1 $aMARTINS, M. F.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrossilvipastoril. Para informações adicionais entre em contato com cpamt.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
03/11/2018 |
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Data da última atualização: |
03/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SOCOLOSKI, S. N. G.; CASTRO, B. G. de; CORDEIRO, M. D.; FONSECA, A. H. da; CEPEDA, M. B.; NICOLINO, R. R.; LOPES, L. B. |
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Afiliação: |
SUYANE NAYARA GARCIA SOCOLOSKI, UFMT, SINOP; BRUNO GOMES DE CASTRO, UFMT, SINOP; MATHEUS DIAS CORDEIRO, UFRRJ, SEROPEDICA; ADIVALDO HENRIQUE DA FONSECA, UFRRJ, SEROPEDICA; MARCIO BARIZON CEPEDA, UFRRJ, SEROPEDICA; RAFAEL ROMERO NICOLINO, UFVJM, CACHOEIRA; LUCIANO BASTOS LOPES, CPAMT. |
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Título: |
Epidemiological investigation of Borrelia burgdorferi in horses in the municipality of Sinop-MT, Brazil. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Tropical Animal Health and Production, v. 50, n. 4, p. 831-836, 2018. |
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Páginas: |
6 p. |
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ISSN: |
0049-4747 |
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Idioma: |
Inglês |
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Conteúdo: |
Borrelia burgdorferi sensu stricto is the main etiological agent of Lyme disease (LD) in the USA. In Brazil, it is believed that a similar spirochete is the causal agent of the Baggio?Yoshinari syndrome (BYS), a zoonosis also transmitted by ticks, whose clinical manifestations are similar to those of LD. Despite the epidemiological importance, there are no studies reporting the presence and the prevalence of B. burgdorferi among horses in Mato Grosso State. The aim of this study was to detect and measure the frequency of IgG antibodies anti-B. burgdorferi American strain G39/40 in horses in the municipality of Sinop, MT?Brazil, using the indirect enzyme-linked immunosorbent assay (ELISA) for serological diagnosis. Blood samples from 367 horses were collected in 81 farms. An epidemiological questionnaire was applied during the visits to obtain information related to the animals and the farms. From the 367 horses, 214 were positive for B. burgdorferi sensu stricto according to the results of the ELISA test, representing an apparent prevalence of 54.04% [CI = 0.4548051?0.6237234]. Concomitantly, 89 blood samples were taken for molecular analysis by nested polymerase chain reaction (PCR). According to the PCR test results, none of the samples were reactive, although 53 of these samples were reactive according to ELISA. Seventy five farms (92.59%) had at least one reactive horse for B. burgdorferi. Our results support the hypothesis of the presence of anti-Borrelia spp. antibodies in horses in Mato Grosso, reaching a high animal prevalence. Besides that, leisure/sport purposes proved to be a risk factor, with an odds ratio of 3.16. These findings clearly indicate the need of borreliosis control in Sinop and make a significant contribution to the knowledge of the disease in Mato Grosso. MenosBorrelia burgdorferi sensu stricto is the main etiological agent of Lyme disease (LD) in the USA. In Brazil, it is believed that a similar spirochete is the causal agent of the Baggio?Yoshinari syndrome (BYS), a zoonosis also transmitted by ticks, whose clinical manifestations are similar to those of LD. Despite the epidemiological importance, there are no studies reporting the presence and the prevalence of B. burgdorferi among horses in Mato Grosso State. The aim of this study was to detect and measure the frequency of IgG antibodies anti-B. burgdorferi American strain G39/40 in horses in the municipality of Sinop, MT?Brazil, using the indirect enzyme-linked immunosorbent assay (ELISA) for serological diagnosis. Blood samples from 367 horses were collected in 81 farms. An epidemiological questionnaire was applied during the visits to obtain information related to the animals and the farms. From the 367 horses, 214 were positive for B. burgdorferi sensu stricto according to the results of the ELISA test, representing an apparent prevalence of 54.04% [CI = 0.4548051?0.6237234]. Concomitantly, 89 blood samples were taken for molecular analysis by nested polymerase chain reaction (PCR). According to the PCR test results, none of the samples were reactive, although 53 of these samples were reactive according to ELISA. Seventy five farms (92.59%) had at least one reactive horse for B. burgdorferi. Our results support the hypothesis of the presence of anti-Borrelia spp. antibodie... Mostrar Tudo |
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Palavras-Chave: |
Borrelia infection; Borreliosis; Enzyme linked immunosorbent assay. |
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Thesagro: |
Elisa. |
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Thesaurus NAL: |
Antibodies; Borrelia burgdorferi; Disease prevalence; Horse diseases; Horses; Lyme disease; Microbiology; Polymerase chain reaction; Prevalence. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02889naa a2200373 a 4500
001 2098720
005 2018-11-03
008 2018 bl uuuu u00u1 u #d
022 $a0049-4747
100 1 $aSOCOLOSKI, S. N. G.
245 $aEpidemiological investigation of Borrelia burgdorferi in horses in the municipality of Sinop-MT, Brazil.$h[electronic resource]
260 $c2018
300 $a6 p.
520 $aBorrelia burgdorferi sensu stricto is the main etiological agent of Lyme disease (LD) in the USA. In Brazil, it is believed that a similar spirochete is the causal agent of the Baggio?Yoshinari syndrome (BYS), a zoonosis also transmitted by ticks, whose clinical manifestations are similar to those of LD. Despite the epidemiological importance, there are no studies reporting the presence and the prevalence of B. burgdorferi among horses in Mato Grosso State. The aim of this study was to detect and measure the frequency of IgG antibodies anti-B. burgdorferi American strain G39/40 in horses in the municipality of Sinop, MT?Brazil, using the indirect enzyme-linked immunosorbent assay (ELISA) for serological diagnosis. Blood samples from 367 horses were collected in 81 farms. An epidemiological questionnaire was applied during the visits to obtain information related to the animals and the farms. From the 367 horses, 214 were positive for B. burgdorferi sensu stricto according to the results of the ELISA test, representing an apparent prevalence of 54.04% [CI = 0.4548051?0.6237234]. Concomitantly, 89 blood samples were taken for molecular analysis by nested polymerase chain reaction (PCR). According to the PCR test results, none of the samples were reactive, although 53 of these samples were reactive according to ELISA. Seventy five farms (92.59%) had at least one reactive horse for B. burgdorferi. Our results support the hypothesis of the presence of anti-Borrelia spp. antibodies in horses in Mato Grosso, reaching a high animal prevalence. Besides that, leisure/sport purposes proved to be a risk factor, with an odds ratio of 3.16. These findings clearly indicate the need of borreliosis control in Sinop and make a significant contribution to the knowledge of the disease in Mato Grosso.
650 $aAntibodies
650 $aBorrelia burgdorferi
650 $aDisease prevalence
650 $aHorse diseases
650 $aHorses
650 $aLyme disease
650 $aMicrobiology
650 $aPolymerase chain reaction
650 $aPrevalence
650 $aElisa
653 $aBorrelia infection
653 $aBorreliosis
653 $aEnzyme linked immunosorbent assay
700 1 $aCASTRO, B. G. de
700 1 $aCORDEIRO, M. D.
700 1 $aFONSECA, A. H. da
700 1 $aCEPEDA, M. B.
700 1 $aNICOLINO, R. R.
700 1 $aLOPES, L. B.
773 $tTropical Animal Health and Production$gv. 50, n. 4, p. 831-836, 2018.
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