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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
16/11/2010 |
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Data da última atualização: |
10/12/2010 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ZEID, M.; YU, J. K.; GOLDOWITZ, I.; DENTON, M. E.; COSTICH, D. E.; JAYASURIYA, C. T.; SAHA, M.; ELSHIRE, R.; BENSCHER, D.; BRESEGHELLO, F.; MUNKVOLD, J.; VARSHNEY, R. K.; BELAY, G.; SORRELLS, M. E. |
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Afiliação: |
M. ZEID, CORNELL UNIVERSITY; J. K. YU, CORNELL UNIVERSITY; I. GOLDOWITZ, CORNELL UNIVERSITY; M. E. DENTON, CORNELL UNIVERSITY; DENISE E. COSTICH, CORNELL UNIVERSITY; C. T. JAYASURIYA, CORNELL UNIVERSITY; M. SAHA, THE SAMUEL ROBERTS NOBLE FOUNDATION; R. ELSHIRE, CORNELL UNIVERSITY; D. BENSCHER, CORNELL UNIVERSITY; FLAVIO BRESEGHELLO, CNPAF; J. MUNKVOLD, BOYCE THOMPSON INSTITUTE; R. K. VARSHNEY, ICRISAT; G. BELAY, ETHIOPIAN INSTITUTE OF AGRICULTURAL RESEARCH; M. E. SORRELLS, CORNELL UNIVERSITY. |
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Título: |
Cross-amplification of EST-derived markers among 16 grass species. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Field Crops Research, Amsterdam, v. 118, n. 1, p. 28-35, Jul. 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
The availability of a large number of expressed sequence tags (ESTs) has facilitated the development of molecular markers in members of the grass family. As these markers are derived from coding sequences, cross-species amplification and transferability is higher than for markers designed from genomic DNA sequences. In this study, 919 EST-based primers developed from seven grass species were assessed for their amplification across a diverse panel of 16 grass species including cereal, turf and forage crops. Out of the 919 primers tested, 89 successfully amplified DNA from one or more species and 340 primers generated PCR amplicons from at least half of the species in the panel. Only 5.2% of the primers tested produced clear amplicons in all 16 species. The majority of the primers (66.9%) were developed from tall fescue and rice and these two species showed amplification rate of 41.6% and 19.0% across the panel, respectively. The highest amplification rate was found for conserved-intron scanning primers (CISP) developed from pearl millet (91%) and sorghum (75%) EST sequences that aligned to rice sequences. The primers with successful amplification identified in this study showed promise in other grass species as demonstrated in differentiating a set of 13 clones of reed canary grass, a species for which very little genomic research has been done. Sequences from the amplified PCR fragments indicated the potential for the transferable CISP markers for comparative mapping purposes. These primer sets can be immediately used for within and across species mapping and will be especially useful for minor grass species with few or no available molecular markers. MenosThe availability of a large number of expressed sequence tags (ESTs) has facilitated the development of molecular markers in members of the grass family. As these markers are derived from coding sequences, cross-species amplification and transferability is higher than for markers designed from genomic DNA sequences. In this study, 919 EST-based primers developed from seven grass species were assessed for their amplification across a diverse panel of 16 grass species including cereal, turf and forage crops. Out of the 919 primers tested, 89 successfully amplified DNA from one or more species and 340 primers generated PCR amplicons from at least half of the species in the panel. Only 5.2% of the primers tested produced clear amplicons in all 16 species. The majority of the primers (66.9%) were developed from tall fescue and rice and these two species showed amplification rate of 41.6% and 19.0% across the panel, respectively. The highest amplification rate was found for conserved-intron scanning primers (CISP) developed from pearl millet (91%) and sorghum (75%) EST sequences that aligned to rice sequences. The primers with successful amplification identified in this study showed promise in other grass species as demonstrated in differentiating a set of 13 clones of reed canary grass, a species for which very little genomic research has been done. Sequences from the amplified PCR fragments indicated the potential for the transferable CISP markers for comparative mapping purpose... Mostrar Tudo |
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Palavras-Chave: |
CISP; EST-SSR. |
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Thesagro: |
Genética molecular; Gramínea; Marcador molecular. |
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Thesaurus Nal: |
Poaceae; Sequence homology. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02615naa a2200361 a 4500
001 1867036
005 2010-12-10
008 2010 bl uuuu u00u1 u #d
100 1 $aZEID, M.
245 $aCross-amplification of EST-derived markers among 16 grass species.
260 $c2010
520 $aThe availability of a large number of expressed sequence tags (ESTs) has facilitated the development of molecular markers in members of the grass family. As these markers are derived from coding sequences, cross-species amplification and transferability is higher than for markers designed from genomic DNA sequences. In this study, 919 EST-based primers developed from seven grass species were assessed for their amplification across a diverse panel of 16 grass species including cereal, turf and forage crops. Out of the 919 primers tested, 89 successfully amplified DNA from one or more species and 340 primers generated PCR amplicons from at least half of the species in the panel. Only 5.2% of the primers tested produced clear amplicons in all 16 species. The majority of the primers (66.9%) were developed from tall fescue and rice and these two species showed amplification rate of 41.6% and 19.0% across the panel, respectively. The highest amplification rate was found for conserved-intron scanning primers (CISP) developed from pearl millet (91%) and sorghum (75%) EST sequences that aligned to rice sequences. The primers with successful amplification identified in this study showed promise in other grass species as demonstrated in differentiating a set of 13 clones of reed canary grass, a species for which very little genomic research has been done. Sequences from the amplified PCR fragments indicated the potential for the transferable CISP markers for comparative mapping purposes. These primer sets can be immediately used for within and across species mapping and will be especially useful for minor grass species with few or no available molecular markers.
650 $aPoaceae
650 $aSequence homology
650 $aGenética molecular
650 $aGramínea
650 $aMarcador molecular
653 $aCISP
653 $aEST-SSR
700 1 $aYU, J. K.
700 1 $aGOLDOWITZ, I.
700 1 $aDENTON, M. E.
700 1 $aCOSTICH, D. E.
700 1 $aJAYASURIYA, C. T.
700 1 $aSAHA, M.
700 1 $aELSHIRE, R.
700 1 $aBENSCHER, D.
700 1 $aBRESEGHELLO, F.
700 1 $aMUNKVOLD, J.
700 1 $aVARSHNEY, R. K.
700 1 $aBELAY, G.
700 1 $aSORRELLS, M. E.
773 $tField Crops Research, Amsterdam$gv. 118, n. 1, p. 28-35, Jul. 2010.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 17 | |
| 1. |  | GOUVEA, E. G.; BERTIOLI, S. C. de M. L.; PENMETSA, V.; COOK, D.; SENTHILVEL, S.; VARSHNEY, R. K.; BERTIOLI, D. J.; MORETZSOHN, M. de C. Development of genetic linkage maps for the A and B genomes of Arachis using RIL populations. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. 90| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 2. | | BERTIOLI, D. J.; BERTIOLI, S. C. de M. L.; VARSHNEY, R. K.; COOK, D.; PANMETSA, R. V.; PATERSON, A.; GUIMARAES, P. M.; MORETZSOHN, M. de C. Reference genetic resources for peanut. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY, 4, 2009, Mali, África. Advances in Arachis through genomics and biotechnology (AAGB-2009). Mali: International Crops Research Institute for the Semi-Arid Tropics, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 3. | | FROENICKE, L.; PANDEY, M.; UPADHYAYA, H.; MORETZSOHN, M. de C.; GUIMARAES, P. M.; BERTIOLI, S. C. de M. L.; VARSHNEY, R. K.; BERTIOLI, D. J.; MICHELMORE, R. W. Towards ultra-dense genetic maps of peanut generated by sequencing diploid and tetraploid RIL populations and a peanut diversity panel. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 57| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | PANDEY, M. K.; MONYO, E.; OZIAS AKINS, P.; LIANG, X.; GUIMARAES, P. M.; NIGAM, S. N.; UPADHYAYA, H. D.; JANILA, P.; ZHANG, X.; GUO, B.; COOK, D. R.; BERTIOLI, D. J.; MICHELMORE, R.; VARSHNEY, R. K. Advances in Arachis genomics for peanut improvement. Biotechnology Advances, V. 30, P. 639-651, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 5. | | ZEID, M.; YU, J. K.; GOLDOWITZ, I.; DENTON, M. E.; COSTICH, D. E.; JAYASURIYA, C. T.; SAHA, M.; ELSHIRE, R.; BENSCHER, D.; BRESEGHELLO, F.; MUNKVOLD, J.; VARSHNEY, R. K.; BELAY, G.; SORRELLS, M. E. Cross-amplification of EST-derived markers among 16 grass species. Field Crops Research, Amsterdam, v. 118, n. 1, p. 28-35, Jul. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 6. | | VARSHNEY, R. K.; BERTIOLI, D. J.; MORETZSOHN, M. de C.; KOPPOLU, R.; VALDEZ, V.; KRISHNAMURTHY, L.; ARUNA, R.; NIGAM, S. N.; MOSS, B. J.; SEETHA, K.; GUOHAO, H.; KNAPP, S. J.; HOISINGTON, D. A. The first SSR-based genetic linkage map for cultivated groundnut (Arachis hypogaea L.). In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 17., 2009, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2009. P. 359| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | VARSHNEY, R. K.; BERTIOLI, D. J.; MORETZSOHN, M. de C.; VALDEZ, V.; KRISHNAMURTHY, L.; ARUNA, R.; NIGAM, S. N.; MOSS, B. J.; SEETHA, K.; RAVI, K.; HE, G.; KNAPP, S. J.; HOISINGTON, D. A. The first SSR-based genetic linkage map for cultivated groundnut (Arachis hypogaea L.). Theoretical and Applied Genetics, n.118, p. 729-739, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | BERTIOLI, S. C. de M. L.; GUIMARAES, P. M.; BRASILEIRO, A. C. M.; PROITE, K.; ARAUJO, A. C. G. de; MORETZSOHN, M. de C.; VARSHNEY, R. K.; BERTIOLI, D. J. Disease resistance characterization in wild Arachis: microscopy, gene expression analyses and genetic mapping. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY, 4, 2009, Mali, África. Advances in Arachis through genomics and biotechnology (AAGB-2009). Mali: International Crops Research Institute for the Semi-Arid Tropics, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | BERTIOLI, D. J.; MORETZSOHN, M. de C.; BERTIOLI, S. C. de M. L.; NIELEN, S.; ARAUJO, A. C. G. de; BRASILEIRO, A. C. M.; RATNAPARKHE, M.; KIM, C.; COOK, D.; VARSHNEY, R. K.; PATERSON, A.; GUIMARAES, P. M. Studies on the diploid and tetraploid peanut genomes, and a proposal for a genome project. In: PLANT & ANIMAL GENOMES CONFERENCE, 19., 2011, San Diego. Conference... [S.l.]: International Plant & Animal Genome, 2011. W362.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | BERTIOLI, D. J.; MORETZSOHN, M.; GUIMARÃES, P.; LEAL BERTIOLI, S.; MENDES, D.; VARSHNEY, R. K.; VADES, V.; ARUNA, R.; PATERSON A.; RAMI, J. F.; D'HONT, A.; FONCEKA, D.; SANDAL, N.; HOUGAARD, B.; MADSEN, L.; STOUGAARD, J. The use of wild Arachis for genetics, genomics, and improvement of cultivated peanut. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY, 3, 2008, Hyderabad, India. Advances in Arachis through genomics and biotechnology (AAGB-2008). India: International Crops Research Institute for the Semi-Arid Tropics, 2008. Resumo A-06.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | VARSHNEY, R. K.; BERTIOLI, D. J.; MORETZSOHN, M. C.; RAVI, K.; VADEZ, V.; KRISHNAMURTHY, L.; ARUNA, R.; NIGAM, S. N.; MOSS, B. J.; SEETHA, K.; G, HE; KNAPP, S. J.; HOISINGTON, D. A. SSR-based genetic linkage map for cultivated groudnut (Arachis hypogaea L.) for QTL analysis and comparative mapping. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY, 3, 2008, Hyderabad, India. Advances in Arachis through genomics and biotechnology (AAGB-2008). India: International Crops Research Institute for the Semi-Arid Tropics, 2008. Resumo P-41.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | BERTIOLI, S. C. de M. L.; JOSÉ, A. C. V. F.; ALVES FREITAS, D. M. T.; MORETZSOHN, M. de C.; GUIMARAES, P. M.; NIELEN, S.; VIDIGAL, B. S.; PEREIRA, R. W.; PIKE, J.; FAVERO, A. P.; PARNISKE, M.; VARSHNEY, R. K.; BERTIOLI, D. J. Identification of candidate genome regions controlling disease resistance in Arachis. BMC Plant Biology, v. 9, n. 112, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | GAUTAMI, B.; FONCÉKA, D.; PANDEY, M. K.; MORETZSOHN, M. de C.; SUJAY, V.; QIN, H.; YANBIN, H.; FAYE, I.; XIAOPING, C.; PRAKASH, A. B.; SHAH, T. M.; GOWDA, M. V. C.; NIGAM, S. N.; XUANQIANG, L.; HOISINGTON, D. A.; GUO, B.; BERTIOLI, D. J.; RAMI, J. F.; VARSHNEY, R. K. An international reference consensus genetic map with 897 Marker Loci based on 11 mapping populations for tetraploid groundnut (Arachis hypogaea L.). Plos One, v. 7, n. 7, e41213, 2012. (Open Acess)| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | SHIRASAWA, K.; BERTIOLI, D. J.; VARSHNEY, R. K.; MORETZSOHN, M. de C.; BERTIOLI, S. C. de M. L.; THUDI, M.; PANDEY, M. K.; RAMI, J. F.; FONCÉKA, D.; GOWDA, M. V. C.; QIN, H.; GUO, B.; YANBIN, H.; XUANQIANG, L.; HIRAKAWA, H.; TABATA, S.; ISOBE, S. Integrated consensus map of cultivated peanut and wild relatives reveals structures of the A and B genomes of Arachis and divergence of the legume genomes. DNA Research, V. 20, P. 173-184, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | BERTIOLI, D. J.; CANNON, S. B.; FROENICKE, L.; HUANG, G.; FARMER, A. D.; CANNON, E. K. S.; LIU, X.; GAO, D.; CLEVENGER, J.; DASH, S.; REN, L.; MORETZSOHN, M. C.; SHIRASAWA, K.; HUANG, W.; VIDIGAL, B.; ABERNATHY, B.; NIEDERHUTH, C. E.; UMALE, P.; ARAUJO, A. C. G.; KOZIK, A.; KIM, K. do; BUROW, M. D.; VARSHNEY, R. K.; WANG, X.; ZHANG, X.; BARKLEY, N.; GUIMARAES, P. M.; ISOBE, S.; GUO, B.; LIAO, B.; STALKER, H. T.; SCHMITZ, R. J.; SCHEFFLER, B. E.; BERTIOLI, S. C. M. L.; XUN, X.; JACKSON, S. A.; MICHELMORE, R.; OZIAS-AKINS, P. The genome sequences of Arachis duranensis and Arachis ipaensis, the diploid ancestors of cultivated peanut. Nature Genetics, v. 48, p. 438-446, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | MCCOUCH, S.; BAUTE, G. J.; BRADEEN, J.; BRAMEL, P.; BRETTING, P. K.; BUCKLER, E.; BURKE, J. M.; CHAREST, D.; CLOUTIER, S.; COLE, G.; DEMPEWOLF, H.; DINGKUHN, M.; FEUILLET, C.; GEPTS, P.; GRATTAPAGLIA, D.; GUARINO, L.; JACKSON, S.; KNAPP, S.; LANGRIDGE, P.; LAWTON-RAUH, A.; LIJUA, Q.; LUSTY, C.; MICHAEL, T.; MYLES, S.; NAITO, K.; NELSON, R. L.; PONTAROLLO, R.; RICHARDS, C. M.; RIESEBERG, L.; ROSS-IBARRA , J.; ROUNSLEY, S.; HAMILTON, R. S.; SCHURR, U.; STEIN, N.; TOMOOKA, N.; KNAAP, E. van der; TASSEL, d. van; TOLL, J.; VALLS, J. F. M.; VARSHNEY, R. K.; WARD, J.; WAUGH, R.; WENZL, P.; ZAMIR, D. Feeding the future: we must mine the biodiversity in seed banks to help to overcome food shortages, urge Susan McCouch and colleagues. Nature, v. 499, p. 23-24, 2013.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | BERTIOLI, D. J.; JENKINS, J.; CLEVENGER, J.; DUDCHENKO, O.; GAO, D.; SEIJO, G.; LEAL-BERTIOLI, S. C. M.; REN, L.; FARMER, A. D.; PANDEY, M. K.; SAMOLUK, S. S.; ABERNATHY, B.; AGARWAL, G.; BALLÉN-TABORDA, C.; CAMERON, C.; CAMPBELL, J.; CHAVARRO, C.; CHITIKINENI, A.; CHU, Y.; DASH, S.; BAIDOURI, M. E.; GUO, B.; HUANG, W.; KIM, K. D.; KORANI, W.; LANCIANO, S.; LUI, C. G.; MIROUZE, M.; MORETZSOHN, M. C.; PHAM, M.; SHIN, J. H.; SHIRASAWA, K.; SINHAROY, S.; SREEDASYAM, A.; WEEKS, N. T.; ZHANG, X.; ZHENG, Z.; SUN, Z.; FROENICKE, L.; AIDEN, E. L.; MICHELMORE, R.; VARSHNEY, R. K.; HOLBROOK, C. C.; CANNON, E. K. S.; SCHEFFLER, B. E.; GRIMWOOD, J.; OZIAS-AKINS, P.; CANNON, S. B.; JACKSON, S. A.; SCHMUTZ, J. The genome sequence of segmental allotetraploid peanut Arachis hypogaea. Nature Genetics, v. 51, n. 5, p. 877-884, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 17 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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