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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Cerrados; Embrapa Solos. |
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Data corrente: |
29/01/1996 |
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Data da última atualização: |
04/06/2002 |
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Autoria: |
VARGAS, M. A. T. |
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Título: |
Effect of oxygen on N2 fixation by Spirillum lipoferum. |
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Ano de publicação: |
1977 |
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Fonte/Imprenta: |
[S.l.]: Univeristy of Wisconsin, 1977. |
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Páginas: |
69p. |
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Idioma: |
Inglês |
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Notas: |
Master Thesis. |
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Conteúdo: |
The effect of dissolved oxygen tension (DOT) on the growth rate, growth yield, energy efficiency and cell composition of Spirillum lipoferum was evaluated in stirred batch and continuous culture at pH 6.8 and 30 oC using a growth limiting level of 0.1 electron equivalent (e eq) succinate/1 (0.83 g/1) as the C and e- donor source and N2 or NH4+ as the N source. DOT was controlled using an =2-stat with an autoclavable =2 electrode immersed in the culture, and premixed 1% and 4% =2 in N2 as the O2 supply. The apparent optimum DOT level for N2-fixing growth in batch and continuous culture, both from the standpoint of the rate of growth (minimum doubling time, td, of 4, 6 hr in b a t c h) and the efficiency of N2 fixation (26 and 28 mg N2 fixed/g succinate in batch and continuous culture, respectively), was about 0.0005 atm O2. At this optimum DOT for N2 -fixing growth, the storage organic carbon was estimated indirectly to be 5 to 26% and 2 to 14% (depending on the method of calculation of the cell dry weight as poly-B-hydroxybutyrate (PHB) for batch and continuous culture, respectively. The dissimilatory electron requirement for N2 fixation at the optimum DOT level was 33.3 and 34 e- eq succinate dissimilated/mole cell N, for batch and continuous culture, respectively. Under limiting O2 (e.g. DOT <<0.0005 atm O2), the td increased to 7.0 hr in batch culture; the storage C increased to 19 and 27 e- eq/mole cell N (about 48 and 57% PHD), whereas N2 fixation yield decreased to 18 and 15 mg N2 fixation increased........ MenosThe effect of dissolved oxygen tension (DOT) on the growth rate, growth yield, energy efficiency and cell composition of Spirillum lipoferum was evaluated in stirred batch and continuous culture at pH 6.8 and 30 oC using a growth limiting level of 0.1 electron equivalent (e eq) succinate/1 (0.83 g/1) as the C and e- donor source and N2 or NH4+ as the N source. DOT was controlled using an =2-stat with an autoclavable =2 electrode immersed in the culture, and premixed 1% and 4% =2 in N2 as the O2 supply. The apparent optimum DOT level for N2-fixing growth in batch and continuous culture, both from the standpoint of the rate of growth (minimum doubling time, td, of 4, 6 hr in b a t c h) and the efficiency of N2 fixation (26 and 28 mg N2 fixed/g succinate in batch and continuous culture, respectively), was about 0.0005 atm O2. At this optimum DOT for N2 -fixing growth, the storage organic carbon was estimated indirectly to be 5 to 26% and 2 to 14% (depending on the method of calculation of the cell dry weight as poly-B-hydroxybutyrate (PHB) for batch and continuous culture, respectively. The dissimilatory electron requirement for N2 fixation at the optimum DOT level was 33.3 and 34 e- eq succinate dissimilated/mole cell N, for batch and continuous culture, respectively. Under limiting O2 (e.g. DOT <<0.0005 atm O2), the td increased to 7.0 hr in batch culture; the storage C increased to 19 and 27 e- eq/mole cell N (about 48 and 57% PHD), whereas N2 fixation yield decreased to 18 ... Mostrar Tudo |
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Palavras-Chave: |
FBN; Fixacao biologica de nitrogenio; NBF; Nitrogen fixing bacteria; O2; Oxigen; Spirillum lipoferum. |
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Thesagro: |
Bactéria; Biologia do Solo; Fixação de Nitrogênio; Oxigênio; Solo. |
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Thesaurus Nal: |
nitrogen fixation; oxygen; soil. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02324nam a2200313 a 4500
001 1564904
005 2002-06-04
008 1977 bl uuuu m 00u1 u #d
100 1 $aVARGAS, M. A. T.
245 $aEffect of oxygen on N2 fixation by Spirillum lipoferum.
260 $a[S.l.]: Univeristy of Wisconsin$c1977
300 $a69p.
500 $aMaster Thesis.
520 $aThe effect of dissolved oxygen tension (DOT) on the growth rate, growth yield, energy efficiency and cell composition of Spirillum lipoferum was evaluated in stirred batch and continuous culture at pH 6.8 and 30 oC using a growth limiting level of 0.1 electron equivalent (e eq) succinate/1 (0.83 g/1) as the C and e- donor source and N2 or NH4+ as the N source. DOT was controlled using an =2-stat with an autoclavable =2 electrode immersed in the culture, and premixed 1% and 4% =2 in N2 as the O2 supply. The apparent optimum DOT level for N2-fixing growth in batch and continuous culture, both from the standpoint of the rate of growth (minimum doubling time, td, of 4, 6 hr in b a t c h) and the efficiency of N2 fixation (26 and 28 mg N2 fixed/g succinate in batch and continuous culture, respectively), was about 0.0005 atm O2. At this optimum DOT for N2 -fixing growth, the storage organic carbon was estimated indirectly to be 5 to 26% and 2 to 14% (depending on the method of calculation of the cell dry weight as poly-B-hydroxybutyrate (PHB) for batch and continuous culture, respectively. The dissimilatory electron requirement for N2 fixation at the optimum DOT level was 33.3 and 34 e- eq succinate dissimilated/mole cell N, for batch and continuous culture, respectively. Under limiting O2 (e.g. DOT <<0.0005 atm O2), the td increased to 7.0 hr in batch culture; the storage C increased to 19 and 27 e- eq/mole cell N (about 48 and 57% PHD), whereas N2 fixation yield decreased to 18 and 15 mg N2 fixation increased........
650 $anitrogen fixation
650 $aoxygen
650 $asoil
650 $aBactéria
650 $aBiologia do Solo
650 $aFixação de Nitrogênio
650 $aOxigênio
650 $aSolo
653 $aFBN
653 $aFixacao biologica de nitrogenio
653 $aNBF
653 $aNitrogen fixing bacteria
653 $aO2
653 $aOxigen
653 $aSpirillum lipoferum
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Registro original: |
Embrapa Cerrados (CPAC) |
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| Registros recuperados : 274 | |
| 14. |  | VARGAS, M. A. T.; HUNGRIA, M. Legume inoculation in cerrado soils. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 12., 1999, Foz do Iguacu. Nitrogen fixation: from molecules to crop productivity - proceedings. Dordrechet: Kluwer, 2000. p.587-588. (Current Plant Science and Biotechnology in Agriculture, v.38). Editado por Fabio O. Pedrosa, Mariangela Hungria, Geoffrey Yates, William E. Newton.| Biblioteca(s): Embrapa Cerrados. |
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| 19. | | VARGAS, M. A. T.; DOBEREINER, J. Efeito de níveis crescentes de calagem, manganês, magnésio e boro na simbiose e desenvolvimento vegetativo do Stylosanthes guyanensis. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 9, n. 3, p. 21-28, 1974. (Zootecnia). Título em inglês: The effect of increasing levels of lime, magnesium, manganese and boron on the growth and nitrogen fixation of Stylosanthes guyanensis.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 274 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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