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Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
20/12/2018 |
Data da última atualização: |
27/04/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
VARELI, C. S.; PIZZUTTI, I. R.; GEBLER, L.; CARDOSO, C. D.; GAI, D. S. H.; FONTANA, M. E. Z. |
Afiliação: |
Catiucia S. Vareli, UFSM – Federal University of Santa Maria, Chemistry Department, Center of Research and Analysis of Residues and Contaminants (CEPARC), Campus, 97105-900 Santa Maria, RS, Brazil; Ionara R. Pizzutti, a UFSM – Federal University of Santa Maria, Chemistry Department, Center of Research and Analysis of Residues and Contaminants (CEPARC), Campus, 97105-900 Santa Maria, RS, Brazil; LUCIANO GEBLER, CNPUV; Carmem D. Cardoso, a UFSM – Federal University of Santa Maria, Chemistry Department, Center of Research and Analysis of Residues and Contaminants (CEPARC), Campus, 97105-900 Santa Maria, RS, Brazil; Daniela S.H. Gai, a UFSM – Federal University of Santa Maria, Chemistry Department, Center of Research and Analysis of Residues and Contaminants (CEPARC), Campus, 97105-900 Santa Maria, RS, Brazil; Marlos E.Z. Fontana, a UFSM – Federal University of Santa Maria, Chemistry Department, Center of Research and Analysis of Residues and Contaminants (CEPARC), Campus, 97105-900 Santa Maria, RS, Brazil. |
Título: |
Analytical method validation to evaluate dithiocarbamates degradation in biobeds in South of Brazil. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Talanta, v. 184, p. 202-209, 2018. |
Idioma: |
Inglês |
Conteúdo: |
In order to evaluate the efficiency of biobeds on DTC degradation, the aim of this study was to apply, optimize and validate a method to determine dithiocarbamate (mancozeb) in biobeds using gas chromatography-tandem mass spectrometry (GC-MS). The DTC pesticide mancozeb was hydrolysed in a tin (II) chloride solution at 1.5% in HCl (4 mol L−1 ), during 1 h in a water bath at 80 °C, and the CS2 formed was extracted in isooctane. After cooling, 1 mL of the organic layer was transferred to an auto sampler vial and analyzed by GC-MS. A complete validation study was performed and the following parameters were assessed: linearity of the analytical curve (r2 ), estimated method and instrument limits of detection and limits of quantification (LODm, LODi, LOQm and LOQi, respectively), accuracy (recovery%), precision (RSD%) and matrix effects. Recovery experiments were carried out with a standard spiking solution of the DTC pesticide thiram. Blank biobed (biomixture) samples were spiked at the three levels corresponding to the CS2 concentrations of 1, 3 and 5 mg kg−1 , with seven replicates each (n = 7). The method presented satisfactory accuracy, with recoveries within the range of 89?96% and RSD ≤ 11%. The analytical curves were linear in the concentration range of 0.05?10 µg CS2 mL−1 (r2 > 0.9946). LODm and LOQm were 0.1 and 0.5 mg CS2 kg−1 , respectively, and the calculated matrix effects were not significant (≤ 20%). The validated method was applied to 80 samples (biomixture), from sixteen different biobeds (collected at five sampling times) during fourteen months. Ten percent of samples presented CS2 concentration below the LOD (0.1 mg CS2 kg−1 ) and 49% of them showed results below the LOQ (0.5 mg CS2 kg−1 ), which demonstrates the biobeds capability to degrade DTC. MenosIn order to evaluate the efficiency of biobeds on DTC degradation, the aim of this study was to apply, optimize and validate a method to determine dithiocarbamate (mancozeb) in biobeds using gas chromatography-tandem mass spectrometry (GC-MS). The DTC pesticide mancozeb was hydrolysed in a tin (II) chloride solution at 1.5% in HCl (4 mol L−1 ), during 1 h in a water bath at 80 °C, and the CS2 formed was extracted in isooctane. After cooling, 1 mL of the organic layer was transferred to an auto sampler vial and analyzed by GC-MS. A complete validation study was performed and the following parameters were assessed: linearity of the analytical curve (r2 ), estimated method and instrument limits of detection and limits of quantification (LODm, LODi, LOQm and LOQi, respectively), accuracy (recovery%), precision (RSD%) and matrix effects. Recovery experiments were carried out with a standard spiking solution of the DTC pesticide thiram. Blank biobed (biomixture) samples were spiked at the three levels corresponding to the CS2 concentrations of 1, 3 and 5 mg kg−1 , with seven replicates each (n = 7). The method presented satisfactory accuracy, with recoveries within the range of 89?96% and RSD ≤ 11%. The analytical curves were linear in the concentration range of 0.05?10 µg CS2 mL−1 (r2 > 0.9946). LODm and LOQm were 0.1 and 0.5 mg CS2 kg−1 , respectively, and the calculated matrix effects were not significant (≤ 20%). The validated method was app... Mostrar Tudo |
Palavras-Chave: |
Biobed; Biomixture; Dithiocarbamates; GC-MS. |
Thesaurus Nal: |
Biodegradation; Degradation. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189181/1/Analytical-method-validation-to-evaluat...radation-in-biobeds-in-South-of-Brazil.pdf
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Marc: |
LEADER 02543naa a2200253 a 4500 001 2102339 005 2019-04-27 008 2018 bl uuuu u00u1 u #d 100 1 $aVARELI, C. S. 245 $aAnalytical method validation to evaluate dithiocarbamates degradation in biobeds in South of Brazil.$h[electronic resource] 260 $c2018 520 $aIn order to evaluate the efficiency of biobeds on DTC degradation, the aim of this study was to apply, optimize and validate a method to determine dithiocarbamate (mancozeb) in biobeds using gas chromatography-tandem mass spectrometry (GC-MS). The DTC pesticide mancozeb was hydrolysed in a tin (II) chloride solution at 1.5% in HCl (4 mol L−1 ), during 1 h in a water bath at 80 °C, and the CS2 formed was extracted in isooctane. After cooling, 1 mL of the organic layer was transferred to an auto sampler vial and analyzed by GC-MS. A complete validation study was performed and the following parameters were assessed: linearity of the analytical curve (r2 ), estimated method and instrument limits of detection and limits of quantification (LODm, LODi, LOQm and LOQi, respectively), accuracy (recovery%), precision (RSD%) and matrix effects. Recovery experiments were carried out with a standard spiking solution of the DTC pesticide thiram. Blank biobed (biomixture) samples were spiked at the three levels corresponding to the CS2 concentrations of 1, 3 and 5 mg kg−1 , with seven replicates each (n = 7). The method presented satisfactory accuracy, with recoveries within the range of 89?96% and RSD ≤ 11%. The analytical curves were linear in the concentration range of 0.05?10 µg CS2 mL−1 (r2 > 0.9946). LODm and LOQm were 0.1 and 0.5 mg CS2 kg−1 , respectively, and the calculated matrix effects were not significant (≤ 20%). The validated method was applied to 80 samples (biomixture), from sixteen different biobeds (collected at five sampling times) during fourteen months. Ten percent of samples presented CS2 concentration below the LOD (0.1 mg CS2 kg−1 ) and 49% of them showed results below the LOQ (0.5 mg CS2 kg−1 ), which demonstrates the biobeds capability to degrade DTC. 650 $aBiodegradation 650 $aDegradation 653 $aBiobed 653 $aBiomixture 653 $aDithiocarbamates 653 $aGC-MS 700 1 $aPIZZUTTI, I. R. 700 1 $aGEBLER, L. 700 1 $aCARDOSO, C. D. 700 1 $aGAI, D. S. H. 700 1 $aFONTANA, M. E. Z. 773 $tTalanta$gv. 184, p. 202-209, 2018.
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Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
15/03/1998 |
Data da última atualização: |
06/12/2007 |
Autoria: |
BELTRÃO, N. E. de M. |
Título: |
Compilacao e simula da palestra "Imagem institucional e sua importancia para uma empresa/organizacao", proferida pelo gerente de comunicacao da ODEBRETCT, Sr. Marcio Nepolidoro. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
Campina Grande: EMBRAPA-CNPA, 1997 |
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Nao paginado |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Desempenho; Image; Imagem; Institucional; Institution Premium; Organizacao; Organization; Perfomance; Premiacao. |
Thesagro: |
Pesquisa. |
Thesaurus NAL: |
research. |
Categoria do assunto: |
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Marc: |
LEADER 00755nam a2200253 a 4500 001 1269520 005 2007-12-06 008 1997 bl uuuu u0uu1 u #d 100 1 $aBELTRÃO, N. E. de M. 245 $aCompilacao e simula da palestra "Imagem institucional e sua importancia para uma empresa/organizacao", proferida pelo gerente de comunicacao da ODEBRETCT, Sr. Marcio Nepolidoro. 260 $aCampina Grande: EMBRAPA-CNPA$c1997 300 $aNao paginado 650 $aresearch 650 $aPesquisa 653 $aBrasil 653 $aDesempenho 653 $aImage 653 $aImagem 653 $aInstitucional 653 $aInstitution Premium 653 $aOrganizacao 653 $aOrganization 653 $aPerfomance 653 $aPremiacao
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