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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
Data corrente: |
17/12/2014 |
Data da última atualização: |
09/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; LEAL, C. L. V.; CALLADO, M. del; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. |
Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL. |
Título: |
Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Zygote, v. 23, n. 6, p. 852-862, 2015. |
DOI: |
https://doi.org/10.1017/S0967199414000537 |
Idioma: |
Inglês |
Conteúdo: |
As the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6–70.0% and blastocyst yield of 15.5–24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos. MenosAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic deve... Mostrar Tudo |
Palavras-Chave: |
Bovine; Chemically induced enucleation; Microtubule; Nuclear transfer. |
Thesaurus Nal: |
chromatin. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1018303/1/Chemically-induced-enucleation-of-activated-bovine-oocytes.pdf
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Marc: |
LEADER 02607naa a2200277 a 4500 001 2018303 005 2024-02-09 008 2015 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1017/S0967199414000537$2DOI 100 1 $aSARAIVA, N. Z. 245 $aChemically induced enucleation of activated bovine oocytes$bchromatin and microtubule organization and production of viable cytoplasts.$h[electronic resource] 260 $c2015 520 $aAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6–70.0% and blastocyst yield of 15.5–24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos. 650 $achromatin 653 $aBovine 653 $aChemically induced enucleation 653 $aMicrotubule 653 $aNuclear transfer 700 1 $aOLIVEIRA, C. S. 700 1 $aLEAL, C. L. V. 700 1 $aCALLADO, M. del 700 1 $aVANTINI, R. 700 1 $aMONTEIRO, F. M. 700 1 $aNICIURA, S. C. M. 700 1 $aGARCIA, J. M. 773 $tZygote$gv. 23, n. 6, p. 852-862, 2015.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
17/11/2016 |
Data da última atualização: |
17/11/2016 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SANTOS, D. C. R. dos; MARCHAO, R. L.; CARVALHO, A. M. de; RAMOS, M. L. G.; SOARES, D. dos S.; SILVA, L. P. da; SOUSA, T. R. de; DELVICO, F. M. dos S. |
Afiliação: |
DIVINA CLÉIA RESENDE DOS SANTOS, UNB; ROBELIO LEANDRO MARCHAO, CPAC; ARMINDA MOREIRA DE CARVALHO, CPAC; MARIA LUCRÉCIA GEROSA RAMOS, UNB; DAIANE DOS SANTOS SOARES, UNB; LETICIA PEREIRA DA SILVA, UNB; THAIS RODRIGUES DE SOUSA, UNB; FRANCISCO MARCOS DOS SANTOS DELVICO, CPAC. |
Título: |
Carbono orgânico e nitrogênio total em um latossolo vermelho sob diferentes sistemas integrados no Cerrado. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: [anais]. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2016. FertBio 2016. |
Páginas: |
p. 477. |
Idioma: |
Português |
Palavras-Chave: |
Carbono orgânico; Fertilização; Rotação. |
Thesagro: |
Cerrado; Latossolo Vermelho; Manejo; Nitrogênio; Solo. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/150100/1/CPAC-3.pdf
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Marc: |
LEADER 01130nam a2200289 a 4500 001 2056475 005 2016-11-17 008 2016 bl uuuu u00u1 u #d 100 1 $aSANTOS, D. C. R. dos 245 $aCarbono orgânico e nitrogênio total em um latossolo vermelho sob diferentes sistemas integrados no Cerrado. 260 $aIn: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: [anais]. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2016. FertBio 2016.$c2016 300 $ap. 477. 650 $aCerrado 650 $aLatossolo Vermelho 650 $aManejo 650 $aNitrogênio 650 $aSolo 653 $aCarbono orgânico 653 $aFertilização 653 $aRotação 700 1 $aMARCHAO, R. L. 700 1 $aCARVALHO, A. M. de 700 1 $aRAMOS, M. L. G. 700 1 $aSOARES, D. dos S. 700 1 $aSILVA, L. P. da 700 1 $aSOUSA, T. R. de 700 1 $aDELVICO, F. M. dos S.
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