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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
27/05/1999 |
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Data da última atualização: |
27/05/1999 |
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Autoria: |
VAN ELSAS, J. D.; VAN OVERBEEK, L. S. |
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Título: |
Use of environmentally-triggered promoters for specific differential gene expression in bacterial inoculants in soil and rhizosphere. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Improving plant productivity with rhizosphere bacteria. Australia: CSIRO, p. 238-244, 1994. |
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Páginas: |
p.238-244 |
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Idioma: |
Inglês |
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Conteúdo: |
Different factors in soil and rhizosphere which affect bacterial activity are discussed in the light of the complexity of these two ecosystems. The use of transposon-based promoter probe insertions to obtain environmentally induced expression of beneficial genes for use in biological control as well as impairment of bacterial survival is described. Pseudomonas fluorescens and P. cepacia promoters reacting to wheat root exudates have been identified. One insertion mutant of P. fluorescens was specifically induced by proline, whereas the P. cepacia mutant was induced by leucine. In soil microcosms, induction of gene expression was shown to be rhizospheres of wheat, maize and grass, but not of clover. Possibilities for using the promoters for the induction of expression of beneficial genes (e.g. cryIVB) are considered. |
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Thesagro: |
Bactéria; Inoculante; Rizosfera; Solo. |
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Thesaurus Nal: |
inoculation methods; rhizosphere; soil. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01525naa a2200229 a 4500
001 1621043
005 1999-05-27
008 1994 bl --- 0-- u #d
100 1 $aVAN ELSAS, J. D.
245 $aUse of environmentally-triggered promoters for specific differential gene expression in bacterial inoculants in soil and rhizosphere.
260 $c1994
300 $ap.238-244
520 $aDifferent factors in soil and rhizosphere which affect bacterial activity are discussed in the light of the complexity of these two ecosystems. The use of transposon-based promoter probe insertions to obtain environmentally induced expression of beneficial genes for use in biological control as well as impairment of bacterial survival is described. Pseudomonas fluorescens and P. cepacia promoters reacting to wheat root exudates have been identified. One insertion mutant of P. fluorescens was specifically induced by proline, whereas the P. cepacia mutant was induced by leucine. In soil microcosms, induction of gene expression was shown to be rhizospheres of wheat, maize and grass, but not of clover. Possibilities for using the promoters for the induction of expression of beneficial genes (e.g. cryIVB) are considered.
650 $ainoculation methods
650 $arhizosphere
650 $asoil
650 $aBactéria
650 $aInoculante
650 $aRizosfera
650 $aSolo
700 1 $aVAN OVERBEEK, L. S.
773 $tImproving plant productivity with rhizosphere bacteria. Australia: CSIRO, p. 238-244, 1994.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
