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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
14/05/2004 |
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Data da última atualização: |
14/05/2004 |
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Autoria: |
SALLES, J. F.; SOUZA, F. A. de; VAN ELSAS, J. D. |
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Título: |
Molecular method to assess the diversity of Burkholderia species in environmental. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 68, n.4, p. 1595-1603, 2002 |
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Idioma: |
Inglês |
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Conteúdo: |
In spite of the importance of many members of the genus Burkholderia in the soil microbial community, no direct method to assess the diversity of this genus has been developed so far. The aim of this work was the development of soil DNA-based PCR-denaturing gradient gel electrophoresis (DGGE), a powerful tool for studying the diversity of microbial communities, for detection and analysis of the Burkholderia diversity in soil samples. Primers specific for the genus Burkholderia were developed based on the 16S rDNA gene sequence and were evaluated in PCRs performed with genomic DNAs from Burkholderia and non-Burkholderia species as the templates. The primer system used exhibited good specificity and sensitivity for the majority of established species of the genus Burkholderia. DGGE analysis of the PCR products obtained showed that there were sufficient differences in migration behavior to distinguish the majority of the 14 Burkholderia species tested. Sequence analysis of amplicons generated with soil DNA exclusively revealed sequences affiliated with sequence of Burkholderia species, demonstrating that the PCR-DGGE method is suitable for studying the diversity of this genus in natural settings. A PCR-DGGE analysis of the Burkholderia communities en two grassland plots revealed differences in diversity mainly between bulk and rhizosphere soil samples; the communities in the latter samples produced more complex patterns. |
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Thesaurus Nal: |
Burkholderia. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01923naa a2200157 a 4500
001 1625774
005 2004-05-14
008 2002 bl --- 0-- u #d
100 1 $aSALLES, J. F.
245 $aMolecular method to assess the diversity of Burkholderia species in environmental.
260 $c2002
520 $aIn spite of the importance of many members of the genus Burkholderia in the soil microbial community, no direct method to assess the diversity of this genus has been developed so far. The aim of this work was the development of soil DNA-based PCR-denaturing gradient gel electrophoresis (DGGE), a powerful tool for studying the diversity of microbial communities, for detection and analysis of the Burkholderia diversity in soil samples. Primers specific for the genus Burkholderia were developed based on the 16S rDNA gene sequence and were evaluated in PCRs performed with genomic DNAs from Burkholderia and non-Burkholderia species as the templates. The primer system used exhibited good specificity and sensitivity for the majority of established species of the genus Burkholderia. DGGE analysis of the PCR products obtained showed that there were sufficient differences in migration behavior to distinguish the majority of the 14 Burkholderia species tested. Sequence analysis of amplicons generated with soil DNA exclusively revealed sequences affiliated with sequence of Burkholderia species, demonstrating that the PCR-DGGE method is suitable for studying the diversity of this genus in natural settings. A PCR-DGGE analysis of the Burkholderia communities en two grassland plots revealed differences in diversity mainly between bulk and rhizosphere soil samples; the communities in the latter samples produced more complex patterns.
650 $aBurkholderia
700 1 $aSOUZA, F. A. de
700 1 $aVAN ELSAS, J. D.
773 $tApplied and Environmental Microbiology, Washington$gv. 68, n.4, p. 1595-1603, 2002
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
16/12/2013 |
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Data da última atualização: |
16/12/2013 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MARTINS, M. F. O.; REIS FILHO, W.; PENTEADO, S. do R. C.; NICKELE, M. A.; THOMAZINI, M. J. |
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Afiliação: |
MILA F. O. MARTINS, FUNCEMA/Embrapa Florestas; WILSON REIS FILHO, EPAGRI/Embrapa Florestas; SUSETE DO ROCIO CHIARELLO PENTEADO, CNPF; M.A. NICKELE, UFPR; MARCILIO JOSE THOMAZINI, CNPF. |
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Título: |
Ants associated with Crop-Livestock-Forest Integration Systems (CLFIS) in Ponta Grossa, PR, Brazil. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: SIMPÓSIO DE MIRMECOLOGIA, 21., 2013, Fortaleza. Anais. Fortaleza: UECE, 2013. |
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Páginas: |
1 p. |
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Descrição Física: |
Disponibilizado online. |
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Idioma: |
Inglês |
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Notas: |
Resumo. |
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Palavras-Chave: |
CLFIS; Crop livestock forest integration systems; ILPF; Integração lavoura pecurária floresta. |
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Thesagro: |
Eucalipto; Formiga. |
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Thesaurus NAL: |
Eucalyptus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/94041/1/2013-Susete1-SM-Ants.pdf
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Marc: |
LEADER 00831nam a2200253 a 4500
001 1973903
005 2013-12-16
008 2013 bl uuuu u00u1 u #d
100 1 $aMARTINS, M. F. O.
245 $aAnts associated with Crop-Livestock-Forest Integration Systems (CLFIS) in Ponta Grossa, PR, Brazil.$h[electronic resource]
260 $aIn: SIMPÓSIO DE MIRMECOLOGIA, 21., 2013, Fortaleza. Anais. Fortaleza: UECE$c2013
300 $a1 p.$cDisponibilizado online.
500 $aResumo.
650 $aEucalyptus
650 $aEucalipto
650 $aFormiga
653 $aCLFIS
653 $aCrop livestock forest integration systems
653 $aILPF
653 $aIntegração lavoura pecurária floresta
700 1 $aREIS FILHO, W.
700 1 $aPENTEADO, S. do R. C.
700 1 $aNICKELE, M. A.
700 1 $aTHOMAZINI, M. J.
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Registro original: |
Embrapa Florestas (CNPF) |
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