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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
27/02/2019 |
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Data da última atualização: |
27/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, R. C. U.; LARA, L. A. de C.; CHIARI, L.; BARRIOS, S. C. L.; VALLE, C. B. do; VALERIO, J. R.; TORRES, F. Z. V.; GARCIA, A. A. F.; SOUZA, A. P. de. |
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Afiliação: |
Rebecca Caroline Ulbricht Ferreira, Universidade de Campinas - Unicamp/Centro de Biologia Molecular e Engenharia Genética; Letícia Aparecida de Castro Lara, Universidade de São Paulo - USP/Escola Superior de Agricultura Luiz de Queiroz - ESALQ/Departameto de Genética; LUCIMARA CHIARI, CNPGC; SANZIO CARVALHO LIMA BARRIOS, CNPGC; CACILDA BORGES DO VALLE, CNPGC; JOSE RAUL VALERIO, CNPGC; FABRICIA ZIMERMANN VILELA TORRES, CNPGC; Antonio Augusto Franco Garcia, Universidade de São Paulo - USP/Escola Superior de Agricultura Luiz de Queiroz - ESALQ/Departameto de Genética; Anete Pereira de Souza, Universidade de Campinas - Unicamp/Centro de Biologia Molecular e Engenharia Genética. |
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Título: |
Genetic Mapping With Allele Dosage Information in Tetraploid Urochloa decumbens (Stapf) R. D. Webster Reveals Insights Into Spittlebug (Notozulia entreriana Berg) Resistance. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 10, February, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Urochloa decumbens (Stapf) R. D. Webster is one of the most important African forage grasses in Brazilian beef production. Currently available genetic-genomic resources for this species are restricted mainly due to polyploidy and apomixis. Therefore, crucial genomic-molecular studies such as the construction of genetic maps and the mapping of quantitative trait loci (QTLs) are very challenging and consequently affect the advancement of molecular breeding. The objectives of this work were to (i) construct an integrated U. decumbens genetic map for a full-sibling progeny using GBS-based markers with allele dosage information, (ii) detect QTLs for spittlebug (Notozulia entreriana) resistance, and (iii) seek putative candidate genes involved in defense against biotic stresses. We used the Setaria viridis genome a reference to align GBS reads and selected 4,240 high-quality SNP markers with allele dosage information. Of these markers, 1,000 were distributed throughout nine homologous groups with a cumulative map length of 1,335.09 cM and an average marker density of 1.33 cM. We detected QTLs for resistance to spittlebug, an important pasture insect pest, that explained between 4.66 and 6.24% of the phenotypic variation. These QTLs are in regions containing putative candidate genes related to defense against biotic stresses. Because this is the first genetic map with SNP autotetraploid dosage data and QTL detection in U. decumbens, it will be useful for future evolutionary studies, genome assembly, and other QTL analyses in Urochloa spp. Moreover, the results might facilitate the isolation of spittlebug-related candidate genes and help clarify the mechanism of spittlebug resistance. These approaches will improve selection efficiency and accuracy in U. decumbens molecular breeding and shorten the breeding cycle. MenosUrochloa decumbens (Stapf) R. D. Webster is one of the most important African forage grasses in Brazilian beef production. Currently available genetic-genomic resources for this species are restricted mainly due to polyploidy and apomixis. Therefore, crucial genomic-molecular studies such as the construction of genetic maps and the mapping of quantitative trait loci (QTLs) are very challenging and consequently affect the advancement of molecular breeding. The objectives of this work were to (i) construct an integrated U. decumbens genetic map for a full-sibling progeny using GBS-based markers with allele dosage information, (ii) detect QTLs for spittlebug (Notozulia entreriana) resistance, and (iii) seek putative candidate genes involved in defense against biotic stresses. We used the Setaria viridis genome a reference to align GBS reads and selected 4,240 high-quality SNP markers with allele dosage information. Of these markers, 1,000 were distributed throughout nine homologous groups with a cumulative map length of 1,335.09 cM and an average marker density of 1.33 cM. We detected QTLs for resistance to spittlebug, an important pasture insect pest, that explained between 4.66 and 6.24% of the phenotypic variation. These QTLs are in regions containing putative candidate genes related to defense against biotic stresses. Because this is the first genetic map with SNP autotetraploid dosage data and QTL detection in U. decumbens, it will be useful for future evolutionary studies... Mostrar Tudo |
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Palavras-Chave: |
Allele dosage; Linkage map; Signalgrass; SNP. |
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Thesagro: |
Brachiaria. |
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Thesaurus Nal: |
Polyploidy; Quantitative traits. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/193444/1/Genetic-mapping-with-alele-dosage-information.pdf
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Marc: |
LEADER 02764naa a2200301 a 4500
001 2106558
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008 2019 bl uuuu u00u1 u #d
100 1 $aFERREIRA, R. C. U.
245 $aGenetic Mapping With Allele Dosage Information in Tetraploid Urochloa decumbens (Stapf) R. D. Webster Reveals Insights Into Spittlebug (Notozulia entreriana Berg) Resistance.$h[electronic resource]
260 $c2019
520 $aUrochloa decumbens (Stapf) R. D. Webster is one of the most important African forage grasses in Brazilian beef production. Currently available genetic-genomic resources for this species are restricted mainly due to polyploidy and apomixis. Therefore, crucial genomic-molecular studies such as the construction of genetic maps and the mapping of quantitative trait loci (QTLs) are very challenging and consequently affect the advancement of molecular breeding. The objectives of this work were to (i) construct an integrated U. decumbens genetic map for a full-sibling progeny using GBS-based markers with allele dosage information, (ii) detect QTLs for spittlebug (Notozulia entreriana) resistance, and (iii) seek putative candidate genes involved in defense against biotic stresses. We used the Setaria viridis genome a reference to align GBS reads and selected 4,240 high-quality SNP markers with allele dosage information. Of these markers, 1,000 were distributed throughout nine homologous groups with a cumulative map length of 1,335.09 cM and an average marker density of 1.33 cM. We detected QTLs for resistance to spittlebug, an important pasture insect pest, that explained between 4.66 and 6.24% of the phenotypic variation. These QTLs are in regions containing putative candidate genes related to defense against biotic stresses. Because this is the first genetic map with SNP autotetraploid dosage data and QTL detection in U. decumbens, it will be useful for future evolutionary studies, genome assembly, and other QTL analyses in Urochloa spp. Moreover, the results might facilitate the isolation of spittlebug-related candidate genes and help clarify the mechanism of spittlebug resistance. These approaches will improve selection efficiency and accuracy in U. decumbens molecular breeding and shorten the breeding cycle.
650 $aPolyploidy
650 $aQuantitative traits
650 $aBrachiaria
653 $aAllele dosage
653 $aLinkage map
653 $aSignalgrass
653 $aSNP
700 1 $aLARA, L. A. de C.
700 1 $aCHIARI, L.
700 1 $aBARRIOS, S. C. L.
700 1 $aVALLE, C. B. do
700 1 $aVALERIO, J. R.
700 1 $aTORRES, F. Z. V.
700 1 $aGARCIA, A. A. F.
700 1 $aSOUZA, A. P. de
773 $tFrontiers in Plant Science$gv. 10, February, 2019.
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Embrapa Gado de Corte (CNPGC) |
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| 8. |  | VALLE, C. B. do. Brachiaria: Seleção vs. melhoramento. In: VALERIO, J.R.; MACEDO, M.C.M. Coletânea de seminários técnicos 1988/1991 (Resumos). Campo Grande, MS : EMBRAPA-CNPGC, 1994. p. 23 (EMBRAPA-CNPGC. Documentos, 55). CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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