Data/hora: 21/06/2024 20:53:09

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 24/05/1996
Data da última atualização: | 24/05/1996
Autoria: | VALENZUELA, P.; MORRIS, W. R.; FARAS, A.; LEVINSON, A. F. W.; RUTTER, W. J.
Afiliação: | Departments of biochemistry/biophysics and Microbiology Unicersity University of California, San Francisco.
Título: | Biochemical and biophysical research communications.
Ano de publicação: | 1973
Fonte/Imprenta: | Academic Press, v.53, n.3, p.1036-1041, 1973.
Idioma: | Inglês
Conteúdo: | Previous studies (1,2) have shom that DNA polymerases (sea urchin and E. coli I) and E. coli RNA polymerases are zinc metalloproteins. We report here that DNA-dependent RNA polymerases I and II from rat liver, I, II and III from sea urchin, and RNA-dependent DNA polymerase (reverse transcriptase) from Rous sarcoma virus are inhibited by pre-incubation with the chelating agent, o-phenanthroline. These enzimes are not affected by pre-incubation with the related compound, m-phenanthroline, which is not a metal chelating agent. The data suggest that, in addition to Mg++ or Mn++, the enzymes require a tightly bound metal ion (possibly zinc) for catalytic activity. The DNA-terminal transferse is also inhibited by o-phenanthroline but not m-phenanthroline.
Palavras-Chave: | Enzimas; Escherichia cli.
Thesagro: | DNA; RNA; Zinco.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (16645-1) - ADC - SP - -- - S514 -  - S514 - DIS