Data/hora: 20/06/2024 15:30:56

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 20/04/1999
Data da última atualização: | 16/06/2008
Autoria: | BODDEY, R. M.; SILVA, L. G. da; REIS, V.; ALVES, B. J. R.; URQUIAGA, S.
Título: | Assessment of bacterial nitrogen fixation in grass species.
Ano de publicação: | 2000
Fonte/Imprenta: | In: TRIPLETT, E. (Ed.). Prokayotic nitrogen fixation: a model system for analysis of a biological process. Wymondham: Horizon Scientific, 2000.
Páginas: | p. 705-726.
Idioma: | Inglês
Conteúdo: | A consensus now seems to be forming that the best strategy to develop a technology whereby cereal or grass crops will benefit significantly from plant associated biological nitrogen fixation (BNF) seems to lie in the improvement of existing plant/diazotroph associations. However, only a limited amount of research has been focussed on examining existing associations of N2-fixing micro-organisms with grasses or cereal for BNF inputs and ecological studies of the bacteria that found associated with field-grown plants. In this review we outline the techniques available to quantify BNF contributions to such associations, the problems associated with their application and discuss the existing evidence that indicates in which associations there are benefits of BNF which can be considered agronomically significant. We also discuss briefly the techniques available to enumerate populations of N2-fixing bacteria within plant tissues, giving an example from our studies using the ELISA (enzyme-linked immuno-absorbent assay) technique, the results of which showed very clearly that direct viable counts in semi-solid culture media seriously under-estimates actual bacterial numbers.
Palavras-Chave: | Biological nitrogen fixation; BNF; FBN; Fixação biológica de nitrogênio.
Thesagro: | Gramínea.
Thesaurus Nal: | Azospirillum; grasses.
Categoria do assunto: | --
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (26618-1) - UPC - SP - -- -  -  - 0024151 - DIS