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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
04/01/2019 |
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Data da última atualização: |
17/10/2023 |
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Autoria: |
SOUZA, M. I. L.; OBA, E.; URIBE-VELÁSQUEZ, L. F. |
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Título: |
Niveles periovulatorios del factor de necrosis tumoral alfa (FBF-alfa) en ovejas sincronizadas con progestágenos durante la estación reproductiva. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
In: CONGRESO INTERNACIONAL OVINO Y CAPRINO, 2., 2005, Bogotá. Memorias... Bogotá: Universidad Nacional de Colombia, 2005. 6 f. 1 CD ROM. |
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Idioma: |
Espanhol |
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Conteúdo: |
Resumen: El experimento se realizó en los meses de mayor actividad reproductiva (Febrero y marzo) en una latitud de 22°53?S y una longitud de 46°29?W en Botucatu, Sao Paulo, Brasil, utilizando ovejas de carne con el estro sincronizado por progestágenos (CIDR) por 13 días y tratadas con una aplicación de 500 UI de eCG en la retirada del dispositivo, considerando el día 0 como el día del estro. En este día las hembras ovinas se sometieron a laparoscopia y se realizó punción ovárica para obtener el contenido del fluido folicular (pool de todos los folículos de cada hembra) para la cuantificación del TNF-?. Esta determinación se realizó por medio de un ensayo biológico (ELISA; línea tumoral de fibroblastos de murinos (L929) sensibles al TNF + buffer completo RPMI + solución ATV (Tripsina 0,2% y verseno 0,02%). Los datos se sometieron a estadística descriptiva. TNF-? presentó valores diferentes entre animales (P<0,01; 43,71 a 188,98 pg/mL) resultando en una media de 80,77 ± 31,42 pg/mL. Aunque se observó una amplia variación individual, estos niveles aumentaron en el momento próximo a la ovulación. Este evento fisiológico (Ovulación) es una reacción inflamatoria que conduce a la ruptura de la pared folicular y la liberación del oocito, en respuesta a un proceso inflamatorio agudo, el cual es compatible con el incremento de los niveles de citocinas durante el período periovulatorio, especialmente del TNF-?. Los efectos del sistema inmune son mediados por los cambios del eje hipotálamo-hipofisiario-gonadal y sus acciones directas sobre las células ováricas (Los esteroides gonadales presentes en este momento son importantes moduladores de la respuesta inmune). Abstract: The experiment occurred in high ciclicity months (February and March) to 22°53?S of latitude and 46°29?W of longitude in Botucatu, Sao Paulo, Brazil, using beef sheep with synchronized estrous using progestagens (CIDR) for 13 days and treated with one application of eCG (500 IU) at dispositive withdrawal, considerating d 0 as the oestrous day. In this day the female sheep were submitted at laparoscopy and ovarian follicular punction to obtain follicular fluids content (pool of all follicles of each female) to TNF-? quantification. This determination were realized by biological assay (ELISA; murine fibroblasts tummoral lineage (L929) sensible to the TNF + buffer complete RPMI + ATV solution (tripsine 0.2% and versene 0.02%). Dates were submitted to descriptive statistical analysis. TNF-? presented different values between animals (P<0.01; 43.71 to 188.98 pg/mL) which results in a mean = 80.77 ± 31.42 pg/mL. Although wide individual variation can be observed that these levels are increased in this moment near to ovulation. This physiological event (ovulation) is a inflamatory reaction which will carry to follicular wall rupture and ovocite liberation, in response to the acute inflamatory process, is compatible the increase cytocin levels during this periovulatory period, specially TNF-?. Immune system effects are mediate by hypothalamus-pituitary-gonads axis changes and directs interactions on ovarian cells (the gonadal steroids presents in this moment are important modulators of immune response). MenosResumen: El experimento se realizó en los meses de mayor actividad reproductiva (Febrero y marzo) en una latitud de 22°53?S y una longitud de 46°29?W en Botucatu, Sao Paulo, Brasil, utilizando ovejas de carne con el estro sincronizado por progestágenos (CIDR) por 13 días y tratadas con una aplicación de 500 UI de eCG en la retirada del dispositivo, considerando el día 0 como el día del estro. En este día las hembras ovinas se sometieron a laparoscopia y se realizó punción ovárica para obtener el contenido del fluido folicular (pool de todos los folículos de cada hembra) para la cuantificación del TNF-?. Esta determinación se realizó por medio de un ensayo biológico (ELISA; línea tumoral de fibroblastos de murinos (L929) sensibles al TNF + buffer completo RPMI + solución ATV (Tripsina 0,2% y verseno 0,02%). Los datos se sometieron a estadística descriptiva. TNF-? presentó valores diferentes entre animales (P<0,01; 43,71 a 188,98 pg/mL) resultando en una media de 80,77 ± 31,42 pg/mL. Aunque se observó una amplia variación individual, estos niveles aumentaron en el momento próximo a la ovulación. Este evento fisiológico (Ovulación) es una reacción inflamatoria que conduce a la ruptura de la pared folicular y la liberación del oocito, en respuesta a un proceso inflamatorio agudo, el cual es compatible con el incremento de los niveles de citocinas durante el período periovulatorio, especialmente del TNF-?. Los efectos del sistema inmune son mediados por los cambios del eje hipotá... Mostrar Tudo |
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Palavras-Chave: |
CIDR; Citocina; Cytocin. |
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Thesagro: |
Ovino; Ovulação; Progesterona. |
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Thesaurus Nal: |
Ovulation; Progesterone; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 04061nam a2200241 a 4500
001 2103061
005 2023-10-17
008 2005 bl uuuu u00u1 u #d
100 1 $aSOUZA, M. I. L.
245 $aNiveles periovulatorios del factor de necrosis tumoral alfa (FBF-alfa) en ovejas sincronizadas con progestágenos durante la estación reproductiva.$h[electronic resource]
260 $aIn: CONGRESO INTERNACIONAL OVINO Y CAPRINO, 2., 2005, Bogotá. Memorias... Bogotá: Universidad Nacional de Colombia, 2005. 6 f. 1 CD ROM.$c2005
520 $aResumen: El experimento se realizó en los meses de mayor actividad reproductiva (Febrero y marzo) en una latitud de 22°53?S y una longitud de 46°29?W en Botucatu, Sao Paulo, Brasil, utilizando ovejas de carne con el estro sincronizado por progestágenos (CIDR) por 13 días y tratadas con una aplicación de 500 UI de eCG en la retirada del dispositivo, considerando el día 0 como el día del estro. En este día las hembras ovinas se sometieron a laparoscopia y se realizó punción ovárica para obtener el contenido del fluido folicular (pool de todos los folículos de cada hembra) para la cuantificación del TNF-?. Esta determinación se realizó por medio de un ensayo biológico (ELISA; línea tumoral de fibroblastos de murinos (L929) sensibles al TNF + buffer completo RPMI + solución ATV (Tripsina 0,2% y verseno 0,02%). Los datos se sometieron a estadística descriptiva. TNF-? presentó valores diferentes entre animales (P<0,01; 43,71 a 188,98 pg/mL) resultando en una media de 80,77 ± 31,42 pg/mL. Aunque se observó una amplia variación individual, estos niveles aumentaron en el momento próximo a la ovulación. Este evento fisiológico (Ovulación) es una reacción inflamatoria que conduce a la ruptura de la pared folicular y la liberación del oocito, en respuesta a un proceso inflamatorio agudo, el cual es compatible con el incremento de los niveles de citocinas durante el período periovulatorio, especialmente del TNF-?. Los efectos del sistema inmune son mediados por los cambios del eje hipotálamo-hipofisiario-gonadal y sus acciones directas sobre las células ováricas (Los esteroides gonadales presentes en este momento son importantes moduladores de la respuesta inmune). Abstract: The experiment occurred in high ciclicity months (February and March) to 22°53?S of latitude and 46°29?W of longitude in Botucatu, Sao Paulo, Brazil, using beef sheep with synchronized estrous using progestagens (CIDR) for 13 days and treated with one application of eCG (500 IU) at dispositive withdrawal, considerating d 0 as the oestrous day. In this day the female sheep were submitted at laparoscopy and ovarian follicular punction to obtain follicular fluids content (pool of all follicles of each female) to TNF-? quantification. This determination were realized by biological assay (ELISA; murine fibroblasts tummoral lineage (L929) sensible to the TNF + buffer complete RPMI + ATV solution (tripsine 0.2% and versene 0.02%). Dates were submitted to descriptive statistical analysis. TNF-? presented different values between animals (P<0.01; 43.71 to 188.98 pg/mL) which results in a mean = 80.77 ± 31.42 pg/mL. Although wide individual variation can be observed that these levels are increased in this moment near to ovulation. This physiological event (ovulation) is a inflamatory reaction which will carry to follicular wall rupture and ovocite liberation, in response to the acute inflamatory process, is compatible the increase cytocin levels during this periovulatory period, specially TNF-?. Immune system effects are mediate by hypothalamus-pituitary-gonads axis changes and directs interactions on ovarian cells (the gonadal steroids presents in this moment are important modulators of immune response).
650 $aOvulation
650 $aProgesterone
650 $aSheep
650 $aOvino
650 $aOvulação
650 $aProgesterona
653 $aCIDR
653 $aCitocina
653 $aCytocin
700 1 $aOBA, E.
700 1 $aURIBE-VELÁSQUEZ, L. F.
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Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
15/05/2007 |
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Data da última atualização: |
31/07/2017 |
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Autoria: |
CIAMPI, M. B.; KURAMAE, E. E.; FENILLE, R. C.; MEYER, M. C.; SOUZA, N. L.; CERESINI, P. C. |
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Título: |
Intraspecific evolution of Rhizoctonia solani AG-1 IA associated with soybean and rice in Brazil based on polymorphisms at the ITS-5.8S rDNA operon. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
European Journal of Plant Pathology, v. 113, n. 2, p. 183-196, Oct. 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
Rhizoctonia solani AG-1 IA causes leaf blight on soybean and rice. Despite the fact that R. solani AG-1 IA is a major pathogen affecting soybean and rice in Brazil and elsewhere in the world, little information is available on its genetic diversity and evolution. This study was an attempt to reveal the origin, and the patterns of movement and amplification of epidemiologically significant genotypes of R. solani AG-1 IA from soybean and rice in Brazil. For inferring intraspecific evolution of R. solani AG-1 IA sampled from soybean and rice, networks of ITS-5.8S rDNA sequencing haplotypes were built using the statistical parsimony algorithm from Clement et al. (2000) Molecular Ecology 9: 1657?1660. Higher haplotype diversity (Nei M 1987, Molecular Evolutionary Genetics Columbia University Press, New york: 512p.) was observed for the Brazilian soybean sample of R. solani AG-1 IA (0.827) in comparison with the rest of the world sample (0.431). Within the south-central American clade (3-2), four haplotypes of R. solani AG-1 IA from Mato Grosso, one from Tocantins, one from Maranhão, and one from Cuba occupied the tips of the network, indicating recent origin. The putative ancestral haplotypes had probably originated either from Mato Grosso or Maranhão States. While 16 distinct haplotypes were found in a sample of 32 soybean isolates of the pathogen, the entire rice sample (n=20) was represented by a single haplotype (haplotype 5), with a worldwide distribution. The results from nested-cladistic analysis indicated restricted gene flow with isolation by distance (or restricted dispersal by distance in nonsexual species) for the south-central American clade (3-2), mainly composed by soybean haplotypes. MenosRhizoctonia solani AG-1 IA causes leaf blight on soybean and rice. Despite the fact that R. solani AG-1 IA is a major pathogen affecting soybean and rice in Brazil and elsewhere in the world, little information is available on its genetic diversity and evolution. This study was an attempt to reveal the origin, and the patterns of movement and amplification of epidemiologically significant genotypes of R. solani AG-1 IA from soybean and rice in Brazil. For inferring intraspecific evolution of R. solani AG-1 IA sampled from soybean and rice, networks of ITS-5.8S rDNA sequencing haplotypes were built using the statistical parsimony algorithm from Clement et al. (2000) Molecular Ecology 9: 1657?1660. Higher haplotype diversity (Nei M 1987, Molecular Evolutionary Genetics Columbia University Press, New york: 512p.) was observed for the Brazilian soybean sample of R. solani AG-1 IA (0.827) in comparison with the rest of the world sample (0.431). Within the south-central American clade (3-2), four haplotypes of R. solani AG-1 IA from Mato Grosso, one from Tocantins, one from Maranhão, and one from Cuba occupied the tips of the network, indicating recent origin. The putative ancestral haplotypes had probably originated either from Mato Grosso or Maranhão States. While 16 distinct haplotypes were found in a sample of 32 soybean isolates of the pathogen, the entire rice sample (n=20) was represented by a single haplotype (haplotype 5), with a worldwide distribution. The results from n... Mostrar Tudo |
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Thesagro: |
Arroz; Fungo; Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02387naa a2200217 a 4500
001 1470001
005 2017-07-31
008 2005 bl --- 0-- u #d
100 1 $aCIAMPI, M. B.
245 $aIntraspecific evolution of Rhizoctonia solani AG-1 IA associated with soybean and rice in Brazil based on polymorphisms at the ITS-5.8S rDNA operon.
260 $c2005
520 $aRhizoctonia solani AG-1 IA causes leaf blight on soybean and rice. Despite the fact that R. solani AG-1 IA is a major pathogen affecting soybean and rice in Brazil and elsewhere in the world, little information is available on its genetic diversity and evolution. This study was an attempt to reveal the origin, and the patterns of movement and amplification of epidemiologically significant genotypes of R. solani AG-1 IA from soybean and rice in Brazil. For inferring intraspecific evolution of R. solani AG-1 IA sampled from soybean and rice, networks of ITS-5.8S rDNA sequencing haplotypes were built using the statistical parsimony algorithm from Clement et al. (2000) Molecular Ecology 9: 1657?1660. Higher haplotype diversity (Nei M 1987, Molecular Evolutionary Genetics Columbia University Press, New york: 512p.) was observed for the Brazilian soybean sample of R. solani AG-1 IA (0.827) in comparison with the rest of the world sample (0.431). Within the south-central American clade (3-2), four haplotypes of R. solani AG-1 IA from Mato Grosso, one from Tocantins, one from Maranhão, and one from Cuba occupied the tips of the network, indicating recent origin. The putative ancestral haplotypes had probably originated either from Mato Grosso or Maranhão States. While 16 distinct haplotypes were found in a sample of 32 soybean isolates of the pathogen, the entire rice sample (n=20) was represented by a single haplotype (haplotype 5), with a worldwide distribution. The results from nested-cladistic analysis indicated restricted gene flow with isolation by distance (or restricted dispersal by distance in nonsexual species) for the south-central American clade (3-2), mainly composed by soybean haplotypes.
650 $aArroz
650 $aFungo
650 $aSoja
700 1 $aKURAMAE, E. E.
700 1 $aFENILLE, R. C.
700 1 $aMEYER, M. C.
700 1 $aSOUZA, N. L.
700 1 $aCERESINI, P. C.
773 $tEuropean Journal of Plant Pathology$gv. 113, n. 2, p. 183-196, Oct. 2005.
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