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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
07/02/2022 |
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Data da última atualização: |
07/02/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TAIRA, A. R.; BATISTA, R. I. T. P.; SANTOS, J. D. R.; PINTO, P. H. N.; BALARO, M. F. A.; ESPÍRITO SANTO, C. G. do; BRAIR, V. L.; SOUZA-FABJAN, J. M. G.; UNGERFELD, R.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
AUGUSTO RYONOSUKE TAIRA, Universidade Federal Fluminense - Niterói, RJ, Brazil; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense - Niterói, RJ, Brazil; JULIANA DANTAS RODRIGUES SANTOS, Universidade Federal Fluminense - Niterói, RJ, Brazil; PEDRO HENRIQUE NICOLAU PINTO, Universidade Federal Fluminense - Niterói, RJ, Brazil; MARIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense - Niterói, RJ, Brazil; CAROLINE GOMES DO ESPÍRITO SANTO, Universidade Federal Fluminense - Niterói, RJ, Brazil; VIVIANE LOPES BRAIR, Universidade Federal Fluminense - Niterói, RJ, Brazil; JOANNA MARIA GONÇALVES SOUZA-FABJAN; RODOLFO UNGERFELD, Universidad de la República - Montevideo, Uruguay; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense - Niterói, RJ, Brazil. |
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Título: |
Progestogen supplementation during superovulation leads to higher embryo viability and TGFB1 gene expression in sheep. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 238, 106938, Mar. 2022. |
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DOI: |
https://doi.org/10.1016/j.anireprosci.2022.106938 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study aimed to compare the effect of the administration of either medroxyprogesterone acetate (MPA) or progesterone (P4) in superovulation (SOV) treatments applied during the first follicular wave on follicular development, embryo yield, and the expression of genes related to pluripotency maintenance, differentiation of the trophectoderm, cell growth and differentiation, apoptosis and energy metabolism in sheep embryos. The estrous cycle of 36 multiparous ewes was synchronized with a short protocol, and the animals were randomly allocated to three groups. At the beginning of SOV, 12 ewes per treatment received an intravaginal sponge impregnated with 60 mg of MPA (TMPA), or an intravaginal device containing 0.33 g of P4 (TP4), or received no progestogen treatment (CON). The device was kept until the fifth dose of FSH. Ewes were mated with five fertile rams. Gene expression was performed by RT-qPCR using grade I and II blastocysts. The numbers of corpora lutea, total structures and viable embryos recovered per ewe were similar (P > 0.05) among groups. However, the viability rate was higher in TP4 (71.9 ± 16.3%) compared to CON (24.4 ± 16.8%; P = 0.01) and similar to TMPA (49.9 ± 16.3%; P = 0.2). Similarly, when compared with CON, treatment with P4 or MPA positively regulated the TGFB1 transcript involved in cell proliferation and differentiation (P = 0.01 and P = 0.03, respectively). In conclusion, supplementation with P4 during the first follicular wave of the estrous cycle improves embryo viability and alters the expression of the TGFB1 gene. MenosAbstract: This study aimed to compare the effect of the administration of either medroxyprogesterone acetate (MPA) or progesterone (P4) in superovulation (SOV) treatments applied during the first follicular wave on follicular development, embryo yield, and the expression of genes related to pluripotency maintenance, differentiation of the trophectoderm, cell growth and differentiation, apoptosis and energy metabolism in sheep embryos. The estrous cycle of 36 multiparous ewes was synchronized with a short protocol, and the animals were randomly allocated to three groups. At the beginning of SOV, 12 ewes per treatment received an intravaginal sponge impregnated with 60 mg of MPA (TMPA), or an intravaginal device containing 0.33 g of P4 (TP4), or received no progestogen treatment (CON). The device was kept until the fifth dose of FSH. Ewes were mated with five fertile rams. Gene expression was performed by RT-qPCR using grade I and II blastocysts. The numbers of corpora lutea, total structures and viable embryos recovered per ewe were similar (P > 0.05) among groups. However, the viability rate was higher in TP4 (71.9 ± 16.3%) compared to CON (24.4 ± 16.8%; P = 0.01) and similar to TMPA (49.9 ± 16.3%; P = 0.2). Similarly, when compared with CON, treatment with P4 or MPA positively regulated the TGFB1 transcript involved in cell proliferation and differentiation (P = 0.01 and P = 0.03, respectively). In conclusion, supplementation with P4 during the first follicular wave of the ... Mostrar Tudo |
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Palavras-Chave: |
Day 0 protocol; Embryo production; Multiple ovulations; Progestins. |
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Thesaurus Nal: |
Animal reproduction; Estrous cycle; Ewes; Follicular development; Gene expression; Gene expression regulation; Medroxyprogesterone; Ovarian development; Progesterone; Small ruminants; Superovulation; Synthetic progestogens. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02951naa a2200445 a 4500
001 2139763
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008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.anireprosci.2022.106938$2DOI
100 1 $aTAIRA, A. R.
245 $aProgestogen supplementation during superovulation leads to higher embryo viability and TGFB1 gene expression in sheep.$h[electronic resource]
260 $c2022
520 $aAbstract: This study aimed to compare the effect of the administration of either medroxyprogesterone acetate (MPA) or progesterone (P4) in superovulation (SOV) treatments applied during the first follicular wave on follicular development, embryo yield, and the expression of genes related to pluripotency maintenance, differentiation of the trophectoderm, cell growth and differentiation, apoptosis and energy metabolism in sheep embryos. The estrous cycle of 36 multiparous ewes was synchronized with a short protocol, and the animals were randomly allocated to three groups. At the beginning of SOV, 12 ewes per treatment received an intravaginal sponge impregnated with 60 mg of MPA (TMPA), or an intravaginal device containing 0.33 g of P4 (TP4), or received no progestogen treatment (CON). The device was kept until the fifth dose of FSH. Ewes were mated with five fertile rams. Gene expression was performed by RT-qPCR using grade I and II blastocysts. The numbers of corpora lutea, total structures and viable embryos recovered per ewe were similar (P > 0.05) among groups. However, the viability rate was higher in TP4 (71.9 ± 16.3%) compared to CON (24.4 ± 16.8%; P = 0.01) and similar to TMPA (49.9 ± 16.3%; P = 0.2). Similarly, when compared with CON, treatment with P4 or MPA positively regulated the TGFB1 transcript involved in cell proliferation and differentiation (P = 0.01 and P = 0.03, respectively). In conclusion, supplementation with P4 during the first follicular wave of the estrous cycle improves embryo viability and alters the expression of the TGFB1 gene.
650 $aAnimal reproduction
650 $aEstrous cycle
650 $aEwes
650 $aFollicular development
650 $aGene expression
650 $aGene expression regulation
650 $aMedroxyprogesterone
650 $aOvarian development
650 $aProgesterone
650 $aSmall ruminants
650 $aSuperovulation
650 $aSynthetic progestogens
653 $aDay 0 protocol
653 $aEmbryo production
653 $aMultiple ovulations
653 $aProgestins
700 1 $aBATISTA, R. I. T. P.
700 1 $aSANTOS, J. D. R.
700 1 $aPINTO, P. H. N.
700 1 $aBALARO, M. F. A.
700 1 $aESPÍRITO SANTO, C. G. do
700 1 $aBRAIR, V. L.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aUNGERFELD, R.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
773 $tAnimal Reproduction Science$gv. 238, 106938, Mar. 2022.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 11 | |
| 1. |  | ROSA, R.; BALARO, M. F.; PINTO, P. H.; SANTOS, G. dos; SANTOS, A.; CAVALCANTI, L.; SOUZA-FABJAN, J. M.; FONSECA, J. F. da; UNGERFELD, R.; BRANDÃO, F. Wave follicular synchronization in Santa Inês ewes. In: INTERNATIONAL CONGRESS OF ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tous: Society for Reprocutive Biololy, 2016. p. 485. Abstract PW1513.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 2. | | TAIRA, A. R.; BRANDÃO, F. Z.; BRAIR, V. L.; COSENTINO, I. O.; LEAL, F. S. C.; RIBEIRO, A. C. S.; BALARO, M. F. A.; BATISTA, I. I. T. P.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; UNGERFELD, R. Biostimulation with the ram effect increases the follicle recruitment, ovulatory diameter, and embryo viability rate in superovulated ewes. Theriogenology, v. 181, p. 140-146, Mar. 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 3. | | ARASHIRO, E. K. N.; UNGERFELD, R.; CLARIGET, R. P.; PINTO, P. H. N.; BALARO, M. F. A.; BRAGANÇA, G. M.; RIBEIRO, L. S.; FONSECA, J. F. da; BRANDÃO, F. Z. Early pregnancy diagnosis in ewes by subjective assessment of luteal vascularisation using colour Doppler ultrasonography. Theriogenology, v. 106, p. 247-252, Jan. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 4. | | SOUZA-FABJAN, J. M. G.; ROSA, R. M. da; BALARO, M. F. A.; PINTO, P. H. N.; SANTOS, G. B. dos; ARASHIRO, E. K. N.; FONSECA, J. F. da; UNGERFELD, R.; BRANDÃO, F. Z. Effect of different hormonal combinations on follicular wave emergence and superovulatory response in sheep. Theriogenology, v. 103, p. 24-29, Nov. 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 5. | | ROSA, R. M.; BALARO, M. F. A.; PINTO, P. H. N.; SARAIVA, H. F. R. A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; RIBEIRO, L. S.; FONSECA, J. F. da; UNGERFELD, R.; BRANDÃO, F. Z. Follicular wave synchronization on in vivo embryo production in Santa Ines sheep. Animal Reproduction, v. 13, n. 3, p. 474, Jul./Sept. 2016. Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain,...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 6. | | SANTOS, J. D. R.; UNGERFELD, R.; BALARO, M. F. A.; SOUZA-FABJAN, J. M. G.; COSENTINO, I. O.; BRAIR, V. L.; SOUZA, C. V. de; PINTO, P. H. N.; BADE, A. L. C.; FONSECA, J. F. da; BRANDÃO, F. Z. Transcervical vs. laparotomy embryo collection in ewes: The effectiveness and welfare implications of each technique. Theriogenology, v. 153, p. 112-121, Sept. 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 7. | | TAIRA, A. R.; SANTOS, J. D. R.; COSENTINO, I. O.; BRAIR, V. L.; ESPÍRITO SANTO, C. G. do; PINTO, P. H. N.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; UNGERFELD, R.; BRANDÃO, J. Z. The use of exogenous progesterone in sheep superovulation protocols influences the percentage of viable embryos. Animal Reproduction, v. 17, n. 3, 2020. Edição dos Abstracts of the 34nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2020 (online).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 8. | | RIBEIRO, A. C. S.; TAIRA. A. R.; SANTOS, V. C.; BRAIR, V. L.; LOPES, M. P. F.; BALARO, M. F. A.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; UNGERFELD, R.; BRANDÃO, F. Z. Use of meloxicam with or without dipyrone in non-surgical embryo recovery in hair sheep: Effects on animal welfare. Reproduction in Domestic Animals, v. 58, n. 7, p. 972-981, Jul. 2023.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | RIBEIRO, A. C. S.; PINTO, P. H. N.; SANTOS, J. D. R.; RAMALHO, N. P.; LAEBER, C. C. R.; BALARO, M. F. A.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; UNGERFELD, R.; BRANDÃO, F. Z. The pFSH dose affects the efficiency of in vivo embryo production in Santa Inês ewes. Animal Reproduction Science, v. 64, 107459, May, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 10. | | TAIRA, A. R.; BATISTA, R. I. T. P.; SANTOS, J. D. R.; PINTO, P. H. N.; BALARO, M. F. A.; ESPÍRITO SANTO, C. G. do; BRAIR, V. L.; SOUZA-FABJAN, J. M. G.; UNGERFELD, R.; FONSECA, J. F. da; BRANDÃO, F. Z. Progestogen supplementation during superovulation leads to higher embryo viability and TGFB1 gene expression in sheep. Animal Reproduction Science, v. 238, 106938, Mar. 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | SANTOS, J. D. R.; BATISTA, R. I. T. P.; UNGERFELD, R.; TAIRA, A. R.; ESPÍRITO SANTO, C. G. do; SOUZA-FABJAN, J. M. G.; FERNANDES, D. A. de M.; BALARO, M. F. A.; COSENTINO, I. O.; BRAIR, V. L.; PINTO, P. H. N.; CARVALHO, A. B. da S.; FONSECA, J. F. da; BRANDÃO, F. Z. Hormonal protocol used for cervical dilation in ewes does not affect morphological embryo quality but reduces recovery rate and temporarily alters gene expression. Veterinary Record, e1064, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 11 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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