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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/02/2000 |
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Data da última atualização: |
25/02/2000 |
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Autoria: |
BALATKOVA, V.; TUPY, J. |
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Afiliação: |
Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha. |
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Título: |
Test-tube fertilization in nicoriana tabacum by means of an artificial pollen tube culture. |
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Ano de publicação: |
1968 |
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Fonte/Imprenta: |
Biologia plantarum (Praha), v.10, n.4, p.266-270, 1968. |
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Idioma: |
Inglês |
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Conteúdo: |
Three techniques of the test-tube fertilization by means of an artificial pollen tube culture are described. The essentials for each individual method are: 1. Transfer of pollen tubes from the sugar solution onto the placentae in the test tube. Placing the placentae with ovulesonto pollen tube culture. Precultivation of pollen tubes on small cellophane squares placed on the surface of a semiliquid medium and transfer of cellophane squares along with the pollen culture to the solid medium, over which finally the placentae with ovules are placed. By means of all the three techniques viable seeds were obtained in vitro. In an artificial medium pollen tubes are able to maintain their fertilizing ability even after the 24 hour cultivation, i.e. at the time after the formation of the gametes. |
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Palavras-Chave: |
Cultura de placentas; Cultura de polem; Cultura de tecidos; Fertilizacao in vitro; Pollem culture; Seed production. |
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Thesagro: |
Produção de Sementes. |
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Thesaurus Nal: |
in vitro fertilization; tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01507naa a2200241 a 4500
001 1648676
005 2000-02-25
008 1968 bl --- 0-- u #d
100 1 $aBALATKOVA, V.
245 $aTest-tube fertilization in nicoriana tabacum by means of an artificial pollen tube culture.
260 $c1968
520 $aThree techniques of the test-tube fertilization by means of an artificial pollen tube culture are described. The essentials for each individual method are: 1. Transfer of pollen tubes from the sugar solution onto the placentae in the test tube. Placing the placentae with ovulesonto pollen tube culture. Precultivation of pollen tubes on small cellophane squares placed on the surface of a semiliquid medium and transfer of cellophane squares along with the pollen culture to the solid medium, over which finally the placentae with ovules are placed. By means of all the three techniques viable seeds were obtained in vitro. In an artificial medium pollen tubes are able to maintain their fertilizing ability even after the 24 hour cultivation, i.e. at the time after the formation of the gametes.
650 $ain vitro fertilization
650 $atissue culture
650 $aProdução de Sementes
653 $aCultura de placentas
653 $aCultura de polem
653 $aCultura de tecidos
653 $aFertilizacao in vitro
653 $aPollem culture
653 $aSeed production
700 1 $aTUPY, J.
773 $tBiologia plantarum (Praha)$gv.10, n.4, p.266-270, 1968.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
