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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
01/12/1997 |
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Data da última atualização: |
01/12/1997 |
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Autoria: |
BORNEMAN, J.; SKROCH, P. W.; O'SULLIVAN, K. M.; PALUS, J. A.; RUMJANEK, N. G.; JANSEN, J. L.; NIENHUIS, J.; TRIPLETT, E. W. |
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Título: |
Molecular microbial diversity of an agricultural soil in Wisconsin. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, Wisconsin, v. 62, n. 6, p. 1935-1943, 1996. |
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Idioma: |
Inglês |
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Conteúdo: |
A culture-independent survey of the soil microbial diversity in a clover-grass pasture in souther wisconsin was conducted by sequence analysis of a universal clone library of genes coding for small-subunit rRNA(rDNA). A rapid and efficient method for extraction of DNA from sils which resulted in highly purified DNA wit minimal shearing was developed. universal small-subunit-rRNA primers were used to amplify DNA extracted from the pasture soil. the PCR products were cloned into pGEM-T,and either hypervariable or conseverd regions were sequenced. the relationships of 124 sequences to those of cultured orgnisms of known pylogeny were determined. of the 124 clones sequenced,98.4% were from the domain bacteria. two of the rDNA sequences were derived from eukaryotic organelles. two of the 124 sequences were of nuclear origin, one being fungal and the other a plant sequences. no sequences of the domain ARCHEA were found. within the domain bacteria,three kingdoms were highly representend:the PROTEOBACTERIA (16.1%),the CYTOPHAGA-FLEXIBACTER-BACTEROIDES GROUP(21.8%), and the low-g+c-contet gram-positive group(31.8%). some kingdoms,such as the thermotogales,the dreen nonsulfer groupthe fusobacteria,and the spirochaetes,were absent.a large number of the sequences(39.4%) were distributed among several clades that are not among the major taxa describ by olsen et al.(G.J.olsen,C.R.Woese,and Overbeek,J.Bacteriol.,176:1-6,1994).From the alignments of the sequencedata,distance matrices were calculated to display the enormous microbial diversity foud in this soil in two wals,as phylogenetic trees and as multidimencional-scaling plots. MenosA culture-independent survey of the soil microbial diversity in a clover-grass pasture in souther wisconsin was conducted by sequence analysis of a universal clone library of genes coding for small-subunit rRNA(rDNA). A rapid and efficient method for extraction of DNA from sils which resulted in highly purified DNA wit minimal shearing was developed. universal small-subunit-rRNA primers were used to amplify DNA extracted from the pasture soil. the PCR products were cloned into pGEM-T,and either hypervariable or conseverd regions were sequenced. the relationships of 124 sequences to those of cultured orgnisms of known pylogeny were determined. of the 124 clones sequenced,98.4% were from the domain bacteria. two of the rDNA sequences were derived from eukaryotic organelles. two of the 124 sequences were of nuclear origin, one being fungal and the other a plant sequences. no sequences of the domain ARCHEA were found. within the domain bacteria,three kingdoms were highly representend:the PROTEOBACTERIA (16.1%),the CYTOPHAGA-FLEXIBACTER-BACTEROIDES GROUP(21.8%), and the low-g+c-contet gram-positive group(31.8%). some kingdoms,such as the thermotogales,the dreen nonsulfer groupthe fusobacteria,and the spirochaetes,were absent.a large number of the sequences(39.4%) were distributed among several clades that are not among the major taxa describ by olsen et al.(G.J.olsen,C.R.Woese,and Overbeek,J.Bacteriol.,176:1-6,1994).From the alignments of the sequencedata,distance matrices were ... Mostrar Tudo |
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Thesagro: |
Ecologia; Microrganismo; Solo. |
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Thesaurus Nal: |
microbial ecology; soil; Wisconsin. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02390naa a2200277 a 4500
001 1620945
005 1997-12-01
008 1996 bl uuuu u00u1 u #d
100 1 $aBORNEMAN, J.
245 $aMolecular microbial diversity of an agricultural soil in Wisconsin.
260 $c1996
520 $aA culture-independent survey of the soil microbial diversity in a clover-grass pasture in souther wisconsin was conducted by sequence analysis of a universal clone library of genes coding for small-subunit rRNA(rDNA). A rapid and efficient method for extraction of DNA from sils which resulted in highly purified DNA wit minimal shearing was developed. universal small-subunit-rRNA primers were used to amplify DNA extracted from the pasture soil. the PCR products were cloned into pGEM-T,and either hypervariable or conseverd regions were sequenced. the relationships of 124 sequences to those of cultured orgnisms of known pylogeny were determined. of the 124 clones sequenced,98.4% were from the domain bacteria. two of the rDNA sequences were derived from eukaryotic organelles. two of the 124 sequences were of nuclear origin, one being fungal and the other a plant sequences. no sequences of the domain ARCHEA were found. within the domain bacteria,three kingdoms were highly representend:the PROTEOBACTERIA (16.1%),the CYTOPHAGA-FLEXIBACTER-BACTEROIDES GROUP(21.8%), and the low-g+c-contet gram-positive group(31.8%). some kingdoms,such as the thermotogales,the dreen nonsulfer groupthe fusobacteria,and the spirochaetes,were absent.a large number of the sequences(39.4%) were distributed among several clades that are not among the major taxa describ by olsen et al.(G.J.olsen,C.R.Woese,and Overbeek,J.Bacteriol.,176:1-6,1994).From the alignments of the sequencedata,distance matrices were calculated to display the enormous microbial diversity foud in this soil in two wals,as phylogenetic trees and as multidimencional-scaling plots.
650 $amicrobial ecology
650 $asoil
650 $aWisconsin
650 $aEcologia
650 $aMicrorganismo
650 $aSolo
700 1 $aSKROCH, P. W.
700 1 $aO'SULLIVAN, K. M.
700 1 $aPALUS, J. A.
700 1 $aRUMJANEK, N. G.
700 1 $aJANSEN, J. L.
700 1 $aNIENHUIS, J.
700 1 $aTRIPLETT, E. W.
773 $tApplied and Environmental Microbiology, Wisconsin$gv. 62, n. 6, p. 1935-1943, 1996.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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