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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
01/12/1997 |
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Data da última atualização: |
01/12/1997 |
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Autoria: |
BORNEMAN, J.; SKROCH, P. W.; O'SULLIVAN, K. M.; PALUS, J. A.; RUMJANEK, N. G.; JANSEN, J. L.; NIENHUIS, J.; TRIPLETT, E. W. |
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Título: |
Molecular microbial diversity of an agricultural soil in Wisconsin. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, Wisconsin, v. 62, n. 6, p. 1935-1943, 1996. |
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Idioma: |
Inglês |
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Conteúdo: |
A culture-independent survey of the soil microbial diversity in a clover-grass pasture in souther wisconsin was conducted by sequence analysis of a universal clone library of genes coding for small-subunit rRNA(rDNA). A rapid and efficient method for extraction of DNA from sils which resulted in highly purified DNA wit minimal shearing was developed. universal small-subunit-rRNA primers were used to amplify DNA extracted from the pasture soil. the PCR products were cloned into pGEM-T,and either hypervariable or conseverd regions were sequenced. the relationships of 124 sequences to those of cultured orgnisms of known pylogeny were determined. of the 124 clones sequenced,98.4% were from the domain bacteria. two of the rDNA sequences were derived from eukaryotic organelles. two of the 124 sequences were of nuclear origin, one being fungal and the other a plant sequences. no sequences of the domain ARCHEA were found. within the domain bacteria,three kingdoms were highly representend:the PROTEOBACTERIA (16.1%),the CYTOPHAGA-FLEXIBACTER-BACTEROIDES GROUP(21.8%), and the low-g+c-contet gram-positive group(31.8%). some kingdoms,such as the thermotogales,the dreen nonsulfer groupthe fusobacteria,and the spirochaetes,were absent.a large number of the sequences(39.4%) were distributed among several clades that are not among the major taxa describ by olsen et al.(G.J.olsen,C.R.Woese,and Overbeek,J.Bacteriol.,176:1-6,1994).From the alignments of the sequencedata,distance matrices were calculated to display the enormous microbial diversity foud in this soil in two wals,as phylogenetic trees and as multidimencional-scaling plots. MenosA culture-independent survey of the soil microbial diversity in a clover-grass pasture in souther wisconsin was conducted by sequence analysis of a universal clone library of genes coding for small-subunit rRNA(rDNA). A rapid and efficient method for extraction of DNA from sils which resulted in highly purified DNA wit minimal shearing was developed. universal small-subunit-rRNA primers were used to amplify DNA extracted from the pasture soil. the PCR products were cloned into pGEM-T,and either hypervariable or conseverd regions were sequenced. the relationships of 124 sequences to those of cultured orgnisms of known pylogeny were determined. of the 124 clones sequenced,98.4% were from the domain bacteria. two of the rDNA sequences were derived from eukaryotic organelles. two of the 124 sequences were of nuclear origin, one being fungal and the other a plant sequences. no sequences of the domain ARCHEA were found. within the domain bacteria,three kingdoms were highly representend:the PROTEOBACTERIA (16.1%),the CYTOPHAGA-FLEXIBACTER-BACTEROIDES GROUP(21.8%), and the low-g+c-contet gram-positive group(31.8%). some kingdoms,such as the thermotogales,the dreen nonsulfer groupthe fusobacteria,and the spirochaetes,were absent.a large number of the sequences(39.4%) were distributed among several clades that are not among the major taxa describ by olsen et al.(G.J.olsen,C.R.Woese,and Overbeek,J.Bacteriol.,176:1-6,1994).From the alignments of the sequencedata,distance matrices were ... Mostrar Tudo |
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Thesagro: |
Ecologia; Microrganismo; Solo. |
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Thesaurus Nal: |
microbial ecology; soil; Wisconsin. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02390naa a2200277 a 4500
001 1620945
005 1997-12-01
008 1996 bl uuuu u00u1 u #d
100 1 $aBORNEMAN, J.
245 $aMolecular microbial diversity of an agricultural soil in Wisconsin.
260 $c1996
520 $aA culture-independent survey of the soil microbial diversity in a clover-grass pasture in souther wisconsin was conducted by sequence analysis of a universal clone library of genes coding for small-subunit rRNA(rDNA). A rapid and efficient method for extraction of DNA from sils which resulted in highly purified DNA wit minimal shearing was developed. universal small-subunit-rRNA primers were used to amplify DNA extracted from the pasture soil. the PCR products were cloned into pGEM-T,and either hypervariable or conseverd regions were sequenced. the relationships of 124 sequences to those of cultured orgnisms of known pylogeny were determined. of the 124 clones sequenced,98.4% were from the domain bacteria. two of the rDNA sequences were derived from eukaryotic organelles. two of the 124 sequences were of nuclear origin, one being fungal and the other a plant sequences. no sequences of the domain ARCHEA were found. within the domain bacteria,three kingdoms were highly representend:the PROTEOBACTERIA (16.1%),the CYTOPHAGA-FLEXIBACTER-BACTEROIDES GROUP(21.8%), and the low-g+c-contet gram-positive group(31.8%). some kingdoms,such as the thermotogales,the dreen nonsulfer groupthe fusobacteria,and the spirochaetes,were absent.a large number of the sequences(39.4%) were distributed among several clades that are not among the major taxa describ by olsen et al.(G.J.olsen,C.R.Woese,and Overbeek,J.Bacteriol.,176:1-6,1994).From the alignments of the sequencedata,distance matrices were calculated to display the enormous microbial diversity foud in this soil in two wals,as phylogenetic trees and as multidimencional-scaling plots.
650 $amicrobial ecology
650 $asoil
650 $aWisconsin
650 $aEcologia
650 $aMicrorganismo
650 $aSolo
700 1 $aSKROCH, P. W.
700 1 $aO'SULLIVAN, K. M.
700 1 $aPALUS, J. A.
700 1 $aRUMJANEK, N. G.
700 1 $aJANSEN, J. L.
700 1 $aNIENHUIS, J.
700 1 $aTRIPLETT, E. W.
773 $tApplied and Environmental Microbiology, Wisconsin$gv. 62, n. 6, p. 1935-1943, 1996.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 4 | |
| 1. |  | CARMO, K.; BOURSCHEIDT, M.; BERBER, G.; DIAS, R.; TRIPLETT, E. W.; WEBER, O.; FERREIRA, A. Composição da comunidade bacteriana do solo sob sistemas integrados na região norte de Mato Grosso. In: SEMANA ACADÊMICA - SINOP/2014, 1.; JORNADA CIENTÍFICA DA EMBRAPA AGROSSILVIPASTORIL, 3.; SEMINÁRIO INTEGRADOR PIBID E TUTORIA, MOSTRA DE ENSINO E EXTENSÃO, 1., 2014., Sinop, MT. Resumos... Brasília, DF : Embrapa, 2014. p. 130.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
|    |
| 2. | | BORNEMAN, J.; SKROCH, P. W.; O'SULLIVAN, K. M.; PALUS, J. A.; RUMJANEK, N. G.; JANSEN, J. L.; NIENHUIS, J.; TRIPLETT, E. W. Molecular microbial diversity of an agricultural soil in Wisconsin. Applied and Environmental Microbiology, Wisconsin, v. 62, n. 6, p. 1935-1943, 1996.| Biblioteca(s): Embrapa Agrobiologia. |
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| 3. | | CARMO, K. B. do; DIAS, R.; QUADROS, P. D. de; BERBER, G. C. M.; BOURSCHEIDT, M. L. B.; FARIAS NETO, A. L. de; WEBER, O. L. dos S.; TRIPLETT, E. W.; FERREIRA, A. Assessment of soil bacterial communities in integrated crop production systems within the Amazon Biome, Brazil: a comparative study. Brazilian Journal of Microbiology, 2024. Published: 02 May 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Cerrados. |
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| 4. | | FERREIRA, A.; CARMO, K. B. do; SILVA, J. J. N. da; WEBER, O. L. dos S.; RODRIGUES, R. de A. R.; TRIPLETT, E. W.; BEHLING, M.; FARIAS NETO, A. L. de. Microbiologia de solos em sistemas de integrados de produção no ecótono Cerrado Amazônia. In: FARIAS NETO, A. L. de; NASCIMENTO, A. F. do; ROSSONI, A. L.; MAGALHÃES, C. A. de S.; ITUASSU, D. R.; HOOGERHEIDE, E. S. S.; IKEDA, F. S.; FERNANDES JUNIOR, F.; FARIA, G. R.; ISERNHAGEN, I.; VENDRUSCULO, L. G.; MORALES, M. M.; CARNEVALLI, R. A. (Ed.). Embrapa Agrossilvipastoril: primeiras contribuições para o desenvolvimento de uma agropecuária sustentável. Brasília, DF: Embrapa, 2019. pt. 4, cap. 16, p. 264-268.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agrossilvipastoril; Embrapa Solos. |
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| Registros recuperados : 4 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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