Data/hora: 17/06/2024 07:53:33

Biblioteca(s): | Embrapa Semiárido.
Data corrente: | 31/08/2005
Data da última atualização: | 22/11/2018
Autoria: | TRINDADE, L. C.; LIMA, M. F.; FERREIRA, M. A. S.
Afiliação: | MIRTES FREITAS LIMA, CPATSA.
Título: | Molecular characterization of brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting.
Ano de publicação: | 2005
Fonte/Imprenta: | Fitopatologia Brasileira, Brasília, DF, v. 30, n. 1, p. 46-54, jan./fev. 2005.
Idioma: | Inglês
Conteúdo: | Bacterial canker of grapevine (Vitis vinifera), caused by Xanthomonas campestris pv. viticola was first detected in Brazil in 1998, affecting grapevines in the São Francisco river basin, state of Pernambuco. The disease was also reported in Juazeiro, Bahia and later in Piauí and Ceará. Due to its limited geographical distribution and relatively recent detection in Brazil, very little is known about the pathogen's biology and diversity. Repetitive DNA based-PCR (rep-PCR) profiles were generated from purified bacterial DNA of 40 field strains of X. campestris pv. viticola, collected between 1998 and 2001 in the states of Pernambuco, Bahia and Piauí. Combined analysis of the PCR patterns obtained with primers REP, ERIC and BOX, showed a high degree of similarity among Brazilian strains and the Indian type strain NCPPB 2475. Similar genomic patterns with several diagnostic bands, present in all strains, could be detected. Fingerprints were distinct from those of strains representing other pathovars and from a yellow non-pathogenic isolate from grape leaves. The polymorphism observed among the Brazilian strains allowed their separation into five subgroups, although with no correlation with cultivar of origin, geographic location or year collected.
Palavras-Chave: | Diversidade; DNA repetitivo.
Thesagro: | Cancro Bacteriano; Uva; Vitis Vinifera.
Categoria do assunto: | H Saúde e Patologia
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/186738/1/Fitopatologia-Brasileira-v.30-n.1-p.46-54-2005.pdf
Registro original: | Embrapa Semiárido (CPATSA)
Exemplares: | CPATSA - (31872-1) - UPC - AP - DD -  -  - 10208 - DIS