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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
20/09/2019 |
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Data da última atualização: |
18/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TRICHEZ, D.; STEIDORFF, A. S.; SOARES, C. E. V. F.; FORMIGHIERI, E. F.; ALMEIDA, J. R. M. de. |
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Afiliação: |
Débora Trichez, bolsista da Embrapa Agroenergia.; Andrei S. Steindorff, U.S. Department of Energy (DOE) Joint Genome Institute.; Carlos E. V. F. Soares, UnB; EDUARDO FERNANDES FORMIGHIERI, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
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Título: |
Physiological and comparative genomic analysis of new isolated yeasts Spathaspora sp. JA1 and Meyerozyma caribbica JA9 reveal insights into xylitol production. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
FEMS Yeast Research, v. 19, 2019. |
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Páginas: |
15 p. |
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Idioma: |
Inglês |
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Conteúdo: |
Xylitol is a five-carbon polyol of economic interest that can be produced by microbial xylose reduction from renewable resources. The current study sought to investigate the potential of two yeast strains, isolated from Brazilian Cerrado biome, in the production of xylitol as well as the genomic characteristics that may impact this process. Xylose conversion capacity by the new isolates Spathaspora sp. JA1 and Meyerozyma caribbica JA9 was evaluated and compared with control strains on xylose and sugarcane biomass hydrolysate. Among the evaluated strains, Spathaspora sp. JA1 was the strongest xylitol producer, reaching product yield and productivity as high as 0.74 g/g and 0.20 g/(L.h) on xylose, and 0.58 g/g and 0.44 g/(L.h) on non-detoxified hydrolysate. Genome sequences of Spathaspora sp. JA1 and M. caribbica JA9 were obtained and annotated. Comparative genomic analysis revealed that the predicted xylose metabolic pathway is conserved among the xylitol-producing yeasts Spathaspora sp. JA1, M. caribbica JA9 and Meyerozyma guilliermondii, but not in Spathaspora passalidarum, an efficient ethanol-producing yeast. Xylitol-producing yeasts showed strictly NADPH-dependent xylose reductase and NAD+-dependent xylitol-dehydrogenase activities. This imbalance of cofactors favors the high xylitol yield shown by Spathaspora sp. JA1, which is similar to the most efficient xylitol producers described so far. |
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Palavras-Chave: |
Bagasse hydrolysate; Comparative genomics; Spathaspora; Xylose fermentation. |
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Thesaurus Nal: |
Meyerozyma; Xylitol. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02207naa a2200253 a 4500
001 2112351
005 2019-11-18
008 2019 bl uuuu u00u1 u #d
100 1 $aTRICHEZ, D.
245 $aPhysiological and comparative genomic analysis of new isolated yeasts Spathaspora sp. JA1 and Meyerozyma caribbica JA9 reveal insights into xylitol production.$h[electronic resource]
260 $c2019
300 $a15 p.
520 $aXylitol is a five-carbon polyol of economic interest that can be produced by microbial xylose reduction from renewable resources. The current study sought to investigate the potential of two yeast strains, isolated from Brazilian Cerrado biome, in the production of xylitol as well as the genomic characteristics that may impact this process. Xylose conversion capacity by the new isolates Spathaspora sp. JA1 and Meyerozyma caribbica JA9 was evaluated and compared with control strains on xylose and sugarcane biomass hydrolysate. Among the evaluated strains, Spathaspora sp. JA1 was the strongest xylitol producer, reaching product yield and productivity as high as 0.74 g/g and 0.20 g/(L.h) on xylose, and 0.58 g/g and 0.44 g/(L.h) on non-detoxified hydrolysate. Genome sequences of Spathaspora sp. JA1 and M. caribbica JA9 were obtained and annotated. Comparative genomic analysis revealed that the predicted xylose metabolic pathway is conserved among the xylitol-producing yeasts Spathaspora sp. JA1, M. caribbica JA9 and Meyerozyma guilliermondii, but not in Spathaspora passalidarum, an efficient ethanol-producing yeast. Xylitol-producing yeasts showed strictly NADPH-dependent xylose reductase and NAD+-dependent xylitol-dehydrogenase activities. This imbalance of cofactors favors the high xylitol yield shown by Spathaspora sp. JA1, which is similar to the most efficient xylitol producers described so far.
650 $aMeyerozyma
650 $aXylitol
653 $aBagasse hydrolysate
653 $aComparative genomics
653 $aSpathaspora
653 $aXylose fermentation
700 1 $aSTEIDORFF, A. S.
700 1 $aSOARES, C. E. V. F.
700 1 $aFORMIGHIERI, E. F.
700 1 $aALMEIDA, J. R. M. de
773 $tFEMS Yeast Research$gv. 19, 2019.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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| Registros recuperados : 13 | |
| 1. |  | TRICHEZ, D.; BERGMANN, J. C.; GARCIA, L. C.; JUNGMANN, L. How many bioethanol generations can we have? In: TREICHEL, H.; ALVES JÚNIOR, S. L.; FONGARO, G.; MÜLLER, C. Ethanol as a green alternative fuel: insight and perspectives. Hauppauge, NY: Nova Science Publishers, 2019. cap. 2. p . 21-57.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agroenergia. |
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| 3. | | TRICHEZ, D.; PORTO, B. N.; PACHECO, T. F.; GONCALVES, S. B.; FORMIGHIERI, E. F.; ALMEIDA, J. R. M. de. Physiological and genetic characterization of new Brazilian yeasts for fuels and chemicals production from renewable resources. In: SYMPOSIUM ON THE CHEMISTRY, BIOLOGY AND APPLICATION OF LYTIC POLYSACCHARIDE MONOOXYGENASES, 2016, Copenhagen, Denmark. [Proceedings ...]. Hellerup: Novo Nordisk Fonden, 2016. Não paginado. Resumo nº S10B-1 (P-200).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 4. | | MORAIS JÚNIOR, W. G. de; PACHECO, T. F.; TRICHEZ, D.; ALMEIDA, J. R. M. de; GONCALVES, S. B. Seleção e identificação de nova linhagem de levedura não-Saccharomyces tolerante ao hidrolisado lignocelulósico de cana-de-açúcar para produção de xilitol. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 27.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
|  |
| 5. | | BERGMANN, J. C.; TRICHEZ, D.; SALLET, L. P.; SILVA, F. C. de P.; ALMEIDA, J. R. M. de. Technological advancements in 1G ethanol production and recovery of by-products based on the biorefinery concept. In: CHANDEL, A. K.; SILVEIRA, M. H. L. Advances in sugarcane biorefinery: technologies, commercialization, policy issues and paradigm shift for bioethanol and by-products. Amsterdam: Elsevier, 2018. P. 73-95. Capítulo 4.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agroenergia. |
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| 6. | | TRICHEZ, D.; STEINDORFF, A. S.; SOARES, C. E. V. F.; FORMIGHIERI, E. F.; ALMEIDA, J. R. M. de. Xylitol production: physiological and genetic characterization of new yeast strains. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 2017, 39., San Francisco, US. [Proceedings ...]. Fairfax: Society for Industrial Microbiology and Biotechnology, 2017. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 8. | | TRICHEZ, D.; CARNEIRO, C. V. G. C.; SOARES, C. E. V. F.; STEINDORFF, A. S.; FORMIGHIERI, E. F.; ALMEIDA, J. R. M. de. Biodiversity bioprospecting for xylitol production: selection, physiological and genetic characterization of new yeast strains. In: INTERNATIONAL SPECIALISED SYMPOSIUM ON YEAST - ISSY, 33., 2017, Cork, Ireland. [Proceedings ...]. [S.l]: International Commission on Yeasts, 2017. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 9. | | TRICHEZ, D.; STEINDORFF, A. S.; MORAIS JÚNIOR, W. G. de; VILELA, N.; BERGMANN, J. C.; FORMIGHIERI, E. F.; GONCALVES, S. B.; ALMEIDA, J. R. M. de. Identification of traits to improve co-assimilation of glucose and xylose by adaptive evolution of Spathaspora passalidarum and Scheffersomyces stipitis yeasts. Applied Microbiology and Biotechnology, n. 107, p. 1143?1157, Feb. 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 11. | | CARNEIRO, C. V. G. C.; SERRA, L. A.; PACHECO, T. F.; FERREIRA, L. M. M.; DUARTE, L. T.; FREITAS, M. N. de M.; TRICHEZ, D.; ALMEIDA, J. R. M. de. Advances in Komagataella phaffii Engineering for the Production of Renewable Chemicals and Proteins Fermentation, v. 8, n. 575, p. 2-37, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 12. | | TORRES, N. A. M.; ANDRADE, T. M. G. de; SOUSA, T. D. de; DUARTE-BRANDÃO, L. T.; NAKAI, D. K.; TRICHEZ, D.; CARNEIRO, C. V. G. C.; REIS, V. C. B.; ALMEIDA, J. R. M. de. Engenharia metabólica para a produção de etileno glicol por Komagataella phaffii a partir de hidrolisados de biomassa. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 7., 2023, Brasília, DF. Anais... Brasília, DF : Embrapa, 2023. p. 24.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 13. | | RAMOS, T. G. S.; JUSTEN, F.; CARNEIRO, C. V. G. C.; HONORATO, V. M.; FRANCO, P. F.; VIEIRA, F. S.; TRICHEZ, D.; RODRIGUES, C. M.; ALMEIDA, J. R. M. de. Xylonic acid production by recombinant Komagataella phaffii strains engineered with newly identified xylose dehydrogenases. Bioresource Technology Reports, v. 16, 100825, Dec. 2021. 6 p. PDF: il.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 13 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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