Data/hora: 22/06/2024 00:53:46

Biblioteca(s): | Embrapa Gado de Corte.
Data corrente: | 22/01/2008
Data da última atualização: | 15/08/2023
Autoria: | MIYOSHI, A.; ROSINHA, G. M. S.; CAMARGO I. L. B. C.; TRANT, C. M. C.; CARDOSO, F. C.; AZEVEDO, V.; OLIVEIRA, S. C.
Afiliação: | ANDERSON MIYOSHI, UNIVERSIDADE FEDERAL DE MINAS GERAIS; GRACIA MARIA SOARES ROSINHA, CNPGC; ILANA L. B. C. CAMARGO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; CYNTIA M. C.TRANT, UNIVERSIDADE FEDERAL DE MINAS GERAIS; FERNANDA C. CARDOSO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; VASCO AZEVEDO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; SERGIO C. OLIVEIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS.
Título: | The role of the vacB gene in the pathogenesis of Brucella abortus.
Ano de publicação: | 2007
Fonte/Imprenta: | Microbes and infections, v. 9, n. 3, p. 375-381, Mar. 2007.
DOI: | https://doi.org/10.1016/j.micinf.2006.12.004
Idioma: | Inglês
Conteúdo: | Brucella species are important zoonotic pathogens affecting a wide variety of mammals. Therefore, the identification of new Brucella virulence factors is of great interest in understanding bacterial pathogenesis and immune evasion. In this study, we have identified Brucella abortus vacB gene that presents 2343 nucleotides and 781 amino acids and it shows 39% identity with Shigella flexneri vacB gene that encodes an exoribonuclease RNase R involved in bacterial virulence. Further, we have inactivated Brucella vacB by gene replacement strategy generating a deletion mutant strain. In order to test the role of Brucella vacB in pathogenesis, BALB/c and interferon regulatory factor-1 (IRF-1) knockout (KO) mice received Brucella vacB mutant, the virulent parental strain 2308 or the vaccine strain RB51 and the bacterial CFU numbers in spleens and mous survival were monitored. Our results demonstrated that the B. abortus DvacB mutant and the wild type strain 2308 showed similar CFU numbers in BALB/c mice. Additionally, IRF-1 KO mice that received either the vacB mutant or S2308 strain died in 12e14 days postinfection; in contrast, all animals that received the RB51 vaccine strain survived for 30 days postinoculation. In summary, this study reports that the vacB gene in B. abortus has no impact on bacterial pathogenesis.
Thesagro: | Bovino; Brucella Abortus; Brucelose; Sanidade Animal.
Thesaurus Nal: | Animal health; Cattle; Pathogenesis.
Categoria do assunto: | --
Registro original: | Embrapa Gado de Corte (CNPGC)
Exemplares: | CNPGC - (11699-1) - UPC - AP - -- - 456/03 -  - 456/03 - DIS