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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
26/12/2019 |
Data da última atualização: |
13/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BLECHA, I. M. Z.; SOUSA, I. I.; FERREIRA, A. B. R.; FEIJO, G. L. D.; TORRES JUNIOR, R. A. de A.; SIQUEIRA, F. |
Afiliação: |
Isabella Maiumi Zaidan Blecha, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal; Isadora Inácio Sousa, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal; ANNA BEATRIZ ROBOTTOM FERREIRA, CTAA; GELSON LUIS DIAS FEIJO, CNPGC; ROBERTO AUGUSTO DE A TORRES JUNIOR, CNPGC; FABIANE SIQUEIRA, CNPGC. |
Título: |
Alternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Revista Brasileira de Zootecnia, v. 48, e20180218, 2019. |
Idioma: |
Inglês |
Conteúdo: |
The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform in any basic molecular biology laboratory. If the association of these markers with traits of economic interest in beef cattle is confirmed in new studies, these methodologies may contribute to the selection of animals with superior genetics, i.e., with the potential to produce better-quality meat, either by marker-assisted selection or by the inclusion of these polymorphisms in high-density marker panels. MenosThe objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform i... Mostrar Tudo |
Thesaurus Nal: |
Animal breeding; Marker-assisted selection; Meat tenderness. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207815/1/Alternative-methodologies-for-genotyping.pdf
|
Marc: |
LEADER 02589naa a2200217 a 4500 001 2117678 005 2020-01-13 008 2019 bl uuuu u00u1 u #d 100 1 $aBLECHA, I. M. Z. 245 $aAlternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle.$h[electronic resource] 260 $c2019 520 $aThe objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform in any basic molecular biology laboratory. If the association of these markers with traits of economic interest in beef cattle is confirmed in new studies, these methodologies may contribute to the selection of animals with superior genetics, i.e., with the potential to produce better-quality meat, either by marker-assisted selection or by the inclusion of these polymorphisms in high-density marker panels. 650 $aAnimal breeding 650 $aMarker-assisted selection 650 $aMeat tenderness 700 1 $aSOUSA, I. I. 700 1 $aFERREIRA, A. B. R. 700 1 $aFEIJO, G. L. D. 700 1 $aTORRES JUNIOR, R. A. de A. 700 1 $aSIQUEIRA, F. 773 $tRevista Brasileira de Zootecnia$gv. 48, e20180218, 2019.
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Embrapa Gado de Corte (CNPGC) |
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Registro Completo
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
09/07/2020 |
Data da última atualização: |
09/07/2020 |
Tipo da produção científica: |
Folder/Folheto/Cartilha |
Autoria: |
FERRÃO, R. G.; FERRAO, M. A. G.; FONSECA, A. F. A. da; VOLPI, P. S.; VERDIN FILHO, A. C.; LANI, J. A.; MAURI, A. L.; TÓFFANO, J. L.; TRAGINO, P. H.; BRAVIM, A. J. B.; MORELLI, A. P. |
Afiliação: |
ROMÁRIO GAVA FERRÃO, INCAPER; MARIA AMELIA GAVA FERRAO, CNPCa; AYMBIRÉ FRANCISCO ALMEIDA DA FONSECA, INCAPER; PAULO SÉRGIO VOLPI, INCAPER; ABRAÃO CARLOS VERDIN FILHO, INCAPER; JOSÉ ANTÔNIO LANI, INCAPER; ALDO LUÍS MAURI, INCAPER; JOSÉ LUIZ TÓFFANO, INCAPER; PAULO HENRIQUE TRAGINO, INCAPER; ALONSO JOSÉ BONISSON BRAVIM, INCAPER; ALDEMAR POLONINI MORELLI, INCAPER. |
Título: |
'ES8122' - Jequitibá: nova variedade clonal de café conilon de maturação intermediária para o Espírito Santo. |
Edição: |
2. ed. rev. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Vitória, ES: Incaper, 2015. |
Páginas: |
5 p. |
Série: |
(Incaper. Documentos, 220). |
ISSN: |
1519-2059 |
Idioma: |
Português |
Conteúdo: |
A variedade "ES8122- Jequitibá" foi obtida do Programa de Melhoramento Genético do Incaper em parceria com a Embrapa Café e o apoio do Consórcio Pesquisa Café, com a utilização de diferentes estratégias de melhoramento e de experimentos conduzidos nas fazendas experimentais de Marilândia, Sooretama e Bananal do Norte, localizadas em ambientes representativos de cultivo do café conilon, das regiões noroeste, nordeste e sul do Espírito Santo, respectivamente. |
Palavras-Chave: |
Conilon. |
Thesagro: |
Coffea Canephora; Melhoramento Genético Vegetal; Variedade. |
Thesaurus NAL: |
Coffea canephora var. laurentii; Plant breeding. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/214528/1/02-VARIEDADE-CAFE-JEQUITIBA.pdf
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Marc: |
LEADER 01489nam a2200349 a 4500 001 2123772 005 2020-07-09 008 2015 bl uuuu u0uu1 u #d 022 $a1519-2059 100 1 $aFERRÃO, R. G. 245 $a'ES8122' - Jequitibá$bnova variedade clonal de café conilon de maturação intermediária para o Espírito Santo.$h[electronic resource] 250 $a2. ed. rev. 260 $aVitória, ES: Incaper$c2015 300 $a5 p. 490 $a(Incaper. Documentos, 220). 520 $aA variedade "ES8122- Jequitibá" foi obtida do Programa de Melhoramento Genético do Incaper em parceria com a Embrapa Café e o apoio do Consórcio Pesquisa Café, com a utilização de diferentes estratégias de melhoramento e de experimentos conduzidos nas fazendas experimentais de Marilândia, Sooretama e Bananal do Norte, localizadas em ambientes representativos de cultivo do café conilon, das regiões noroeste, nordeste e sul do Espírito Santo, respectivamente. 650 $aCoffea canephora var. laurentii 650 $aPlant breeding 650 $aCoffea Canephora 650 $aMelhoramento Genético Vegetal 650 $aVariedade 653 $aConilon 700 1 $aFERRAO, M. A. G. 700 1 $aFONSECA, A. F. A. da 700 1 $aVOLPI, P. S. 700 1 $aVERDIN FILHO, A. C. 700 1 $aLANI, J. A. 700 1 $aMAURI, A. L. 700 1 $aTÓFFANO, J. L. 700 1 $aTRAGINO, P. H. 700 1 $aBRAVIM, A. J. B. 700 1 $aMORELLI, A. P.
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